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| 1 | Quantification of Panax notoginseng saponins metabolites in rat plasma with in vivo gut microbiota-mediated biotransformation by HPLC-MS/MS显示文摘Panax notoginseng saponins(PNS) are the major components of Panax notoginseng, with multiple pharmacological activities but poor oral bioavailability. PNS could be metabolized by gut microbiota in vitro, while the exact role of gut microbiota of PNS metabolism in vivo remains poorly understood. In this study, pseudo germ-free rat models were constructed by using broad-spectrum antibiotics to validate the gut microbiota-mediated transformation of PNS in vivo. Moreover, a high performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS/MS) was developed for quantitative analysis of four metabolites of PNS, including ginsenoside F1(GF1), ginsenoside Rh2(GRh2), ginsenoside compound K(GCK) and protopanaxatriol(PPT). The results showed that the four metabolites could be detected in the control rat plasma, while they could not be determined in pseudo germ-free rat plasma. The results implied that PNS could not be biotransformed effectively when gut microbiota was disrupted. In conclusion, gut microbiota plays an important role in biotransformation of PNS into metabolites in vivo. | GUO Yin-Ping CHEN Man-Yun SHAO Li ZHANG Wei RAO Tai ZHOU Hong-Hao HUANG Wei-Hua | 2019 | Chinese Journal of Natural Medicines2019，17，3： | 14 |
| 2 | Panax notoginseng saponins prevent colitis-associated colorectal cancer development: the role of gut microbiota显示文摘Gut microbiota dysbiosis is a risk factor for colorectal cancer(CRC) in inflammatory bowel disease(IBD).In this study, the effects of Panax notoginseng saponins(PNS) on colitis-associated CRC progression were evaluated on an azoxymethane(AOM)/dextran sulfate sodium(DSS) mouse model.In vivo, PNS significantly relieved AOM/DSS-induced colon tumorigenesis and development by reducing the disease activity index(DAI) scores and colon tumor load.The 16S rRNA data of fecal samples showed that the microbiome community was obviously destructed, while PNS could recover the richness and diversity of gut microbiota.Especially, PNS could increase the abundance of Akkermansia spp.which was significantly decreased in model group and negatively correlated with the progression of CRC.Moreover, ginsenoside compound K(GC-K) was evaluated on the effects of human CRC cells,which was the main bio-transformed metabolite of PNS by gut microbiota.Our data showed that PNS played important role in the prevention of the progression of CRC, due to their regulation on the microbiome balance and microbial bio-converted product with antiCRC activity. | CHEN Ling CHEN Man-Yun SHAO Li ZHANG Wei RAO Tai ZHOU Hong-Hao HUANG Wei-Hua | 2020 | Chinese Journal of Natural Medicines2020，18，7： | 12 |
| 3 | The hepatotoxicity of Polygonum multiflorum: The emerging role of the immune-mediated liver injury显示文摘Herbal and dietary supplements(HDS)-induced liver injury has been a great concern all over the world.Polygonum multiflorum Thunb.,a well-known Chinese herbal medicine,is recently drawn increasing attention because of its hepatotoxicity.According to the clinical and experimental studies,P.multiflorum-induced liver injury(PM-DILI)is considered to be immune-mediated idiosyncratic liver injury,but the role of immune response and the underlying mechanisms are not completely elucidated.Previous studies focused on the direct toxicity of PM-DILI by using animal models with intrinsic drug-induced liver injury(DILI).However,most epidemiological and clinical evidence demonstrate that PM-DILI is immune-mediated idiosyncratic liver injury.The aim of this review is to assess current epidemiological,clinical and experimental evidence about the possible role of innate and adaptive immunity in the idiosyncratic hepatotoxicity of P.multiflorum.The potential effects of factors associated with immune tolerance,including immune checkpoint molecules and regulatory immune cells on the individual’s susceptibility to PM-DILI are also discussed.We conclude by giving our hypothesis of possible immune mechanisms of PM-DILI and providing suggestions for future studies on valuable biomarkers identification and proper immune models establishment. | Tai Rao Ya-ting Liu Xiang-chang Zeng Chao-peng Li Dong-sheng Ou-Yang | 2021 | Acta Pharmacologica Sinica2021，42，1： | 11 |
| 4 | Pharmacokinetic and pharmacodynamic evidence for developing an oral formulation of octreotide against gastric mucosal injury显示文摘在 somatostatin 类似物之中, octreotide (10 月) 是通常经由静脉内或下的注射在诊所过去常治疗生长荷尔蒙, gastrin 或胰岛素的增加的分泌物引起的各种各样的疾病的大多数。以便 assesse 开发 10 月的口头的明确的表达的可行性,我们在几个动物模型进行了 10 月的系统的 pharmacokinetic 和 pharmacodynamic 分析。在老鼠的 pharmacokinetic 研究证明 10 月的 intragastric 管理有极其低的 bioavailability (<0.5%) ,但是它能明确地在老鼠胃由于 somatostatin 受体 2 的高表示(SSTR2 ) 散布到胃的 mucosa。pharmacodynamic 研究表明 10 月的 intragastric 管理 dose-dependently 带着胃的溃疡,它比得上那些由 10 月的静脉内的注射完成了的导致 WIRS 的老鼠在老鼠免于胃的 mucosal 损害(GMI ) ,并且这效果被 CYN-154806 的合作管理显著地稀释, SSTR2 的一个对手。在幽门的导致结扎的溃疡老鼠,我们进一步证明那个 10 月显著地减少了胃的酸的分泌物经由下面调整 gastrin 的水平,它为对 GMI 的 10 月的保护的效果负责。总的来说,我们为最重要地开发10月的口头的明确的表达的可行性提供了 pharmacokinetic 和 pharmacodynamic 证据,10月的 pharmacokinetics 和药效学上的 SSTR2 的影响建议10月的口头的明确的表达可能为另外的临床的指示是适用的,在这些肿瘤房间上由于 SSTR2 的 overexpression 包括 neuroendocrine 瘤和垂体腺瘤。 | Xi-nuo LI Tai RAO Yang-fan XU Kang-rui HU Zhang-pei ZHU Hao-feng LI Dian KANG Yu-hao SHAO Bo-yu SHEN Xiao-xi YIN Lin XIE Guang-ji WANG Yan LIANG | 2018 | Acta Pharmacologica Sinica2018，39，8： | 3 |
| 5 | Characterization of patients and its relationship with medical complaints显示文摘 | NI Xin TAI Jun ZHAO Xiao-lan ZHAO Qiong-shu RAO Yuan-sheng HE Jie XU Jian-li GUO Fu-yong FAN Li-hua | 2012 | Chinese Medical Journal2012，，10： | 2 |
| 6 | Appropriate choice of collision-induced dissociation energy for qualitative analysis of notoginsenosides based on liquid chromatography hybrid ion trap time-of-flight mass spectrometry显示文摘Liquid chromatography hybrid ion trap/time-of-flight mass spectrometry possesses both the MS^n ability of ion trap and the excellent resolution of a time-of-flight and has been widely used to identify drug metabolites and determine trace multi-components in natural products. Collision energy, one of the most important factors in acquiring MS^n information, could be set freely in the range of 10%–400%. Herein, notoginsenosides were chosen as model compounds to build a novel methodology for the collision energy optimization. Firstly, the fragmental patterns of the representatives for the authentic standards of protopanaxadiol-type and protopanaxatriol-type notoginsenosides were obtained based on accurate MS^2 and MS^3 measurements via liquid chromatography hybrid ion trap/time-of-flight mass spectrometry. The extracted ion chromatograms of characteristic product ions of notoginsenosides in Panax Notoginseng Extract were produced under a series of collision energies and compared to screen the optimum collision energies values for MS^2 and MS^3. The results demonstrated that the qualitative capability of liquid chromatography hybrid ion trap/time-of-flight mass spectrometry was greatly influenced by collision energies, and 50% of MS^2 collision energy was found to produce the highest collision-induced dissociation efficiency for notoginsenosides. Addtionally, the highest collision-induced dissociation efficiency appeared when the collision energy was set at 75% in the MS^3 stage. | WANG Guang-Ji FU Han-Xu XIAO Jing-Cheng YE Wei RAO Tai SHAO Yu-Hao KANG Dian XIE Lin LIANG Yan | 2016 | Chinese Journal of Natural Medicines2016，14，4： | 2 |
| 7 | Effects of insulin-like growth factor (IGF)-I/IGF-binding protein-3 treatment on glucose metabolism and fat distribution in human immunodeficiency virus-infected patients with abdominal obesity and insulin resistance显示文摘 | Rao MN Mulligan K Tai V | 2010 | JClin Endocrinol Metab2010，95，9： | 1 |
| 8 | Effects of insulin-like growth factor (IGF)-I/IGF-binding protein-3 treatment on glucose metabolism and fat distribution in human immunodefieieney virus-infected patients with abdominal obesity and insulin resistance 显示文摘 | Rao MN Mulligan K Tai V | 2010 | J Clin Endoerinol Metab2010，95，9： | 1 |
| 9 | Effects of insulin-like growth factor(IGF)-I/IGF-binding protein-3 treatment on glucose metabolism and fat distribution in human immunodeficiency virus-infected patients with abdominal obesity and insulin resistance显示文摘 | Rao MN Mulligan K Tai V | 2010 | J Clin Endocrinol Metab2010，95，9： | 1 |
| 10 | TAXAYUNTIN,A NEW TETRACYCLIC TAXANE FROM THE LEAVES OF TAXUS YUNNANENSIS显示文摘A new skeleton taxane diterpene,taxayuntin,was isolated from the leaves and stems ofTaxus yunnanensis.Its structure was determined by spectroscopic methods(MS,1H NMR,13C NMR,1H-1H COSY,1H-13C COSY and 13C-1H COLOC)and X-ray diffraction. | Chang RAO Jin Yun ZHOU Wei Ming CHEN Yang Lu Qi Tai ZHENG Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050, | 1993 | Chinese Chemical Letters1993，4，8： | 0 |
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