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| 1 | K<sup>+</sup> depolarization evokes ATP, adenosine and glutamate release from glia in rat hippocampus: a microelectrode biosensor study显示文摘 | A Heinrich RD Andó G Túri B Rózsa B Sperlágh | 2012 | British Journal of Pharmacology2012，，5： | 1 |
| 2 | Squalamine improves retinal neovascularization显示文摘 | Higgins RD Sander RI Yan Y | 2000 | Invest Ophthalmol Vis Sci2000，41，6： | 1 |
| 3 | Randomized controlled trial comparing endoscopic clips and over-the-scope clips for closure of natural orifice transluminal endoscopic surgery gastrotomies 显示文摘 | yon RD Vassiliou MC Rothstein RI | 2009 | Endoscopy2009，41，： | 1 |
| 4 | Overtraining affects male reproductive status显示文摘 | Roberts AC McClure RD Weiner RI | 1993 | Fertil Steril1993，60，4： | 1 |
| 5 | Storm water runoff mitigation using a green roof显示文摘 | Bliss DJ Neufeld RD Ries RJ | 2009 | Environmental Engineering Science2009，26，2： | 1 |
| 6 | Moving Mass Trim Control for Aerospace Vehicles显示文摘 | RD RI BR S SA K | 1996 | Journal of Guidance Control and Dynamics1996，19，5： | 1 |
| 7 | Effect of chemical vapor infiltration on erosion and thermal properties of porous carbon/carbon composite thermal insulation显示文摘 | BAXTER RI RAWLINGS RD IWASHITA N | 2000 | Carbon2000，38，： | 1 |
| 8 | Measles immune suppression:functional impairment or numbers game? 显示文摘 | De ~ries RD De Swart RL | 2014 | PLoS Pathog2014，10，10： | 1 |
| 9 | Overtraining affects male reproductive status 显示文摘 | Roberts AC McClure RD Weiner RI | 1993 | Fertil Steril1993，60，4： | 1 |
| 10 | Mucosal erosions in longterm non-steroidal anti- inflammatory drug users: predisposition to ulceration and relation to HP显示文摘 | Taha AS Sturrock RD Russell RI | 1995 | Gut1995，36，： | 1 |
| 11 | Effect of herpesvirus infection on pancreatic duct cell secretion显示文摘AIM: To examine the effect of acute infection caused by herpesvirus (pseudorabies virus, PRV) on pancreatic ductal secretion.METHODS: The virulent Ba-DupGreen (BDG) and nonvirulent Ka-RREpOlacgfp (KEG) genetically modified strains of PRV were used in this study and both of them contain the gene for green fluorescent protein (GFP). Small intra/interlobular ducts were infected with BDG virus (107 PFU/mL for 6 h) or with KEG virus (1010 PFU/mL for 6 h), while non-infected ducts were incubated only with the culture media. The ducts were then cultured for a further 18 h.The rate of HCO3- secretion [base efflux -J(B-)] was determined from the buffering capacity of the cells and the initial rate of intracellular acidification (1) after sudden blockage of basolateral base loaders with dihydro-4,4,-diisothiocyanatostilbene-2,2,-disulfonic acid (500 μmol/L)and amiloride (200 μmol/L), and (2) after alkali loading the ducts by exposure to NH4Cl. All the experiments were performed in HCO3--buffered Ringer solution at 37 ℃ (n = 5ducts for each experimental condition). Viral structural proteins were visualized by immunohistochemistry. Virallyencoded GFP and immunofluorescence signals were recorded by a confocal laser scanning microscope.RESULTS: The BDG virus infected the majority of accessible cells of the duct as judged by the appearance of GFP and viral antigens in the ductal cells. KEG virus caused a similarly high efficiency of infection. After blockage of basolateral base loaders, BDG infection significantly elevated -J(B-) 24 h after the infection, compared to the non-infected group. However, KEG infection did not modify -J(B-). After alkali loading the ducts, -J(B-) was significantly elevated in the BDG group compared to the control group 24 h after the infection. As we found with the inhibitor stop method, no change was observed in the group KEG compared to the non-infected group.CONCLUSION: Incubation with the BDG or KEG strains of PRV results in an effective infection of ductal epithelial cells. The BDG strain of PRV, which is able to initiate a lytic viral cycle, stimulates HcO3- secretion in guinea pig pancreatic duct by about four- to fivefold, 24 h after the infection. However, the KEG strain of PRV, which can infect,but fails to replicate, has no effect on HCO3- secretion.We suggest that this response of pancreatic ducts to virulent PRV infection may represent a defense mechanism against invasive pathogens to avoid pancreatic injury. | Péter Hegyi Balázs rdg Zoltán Rakonczai Jr Tamás Takács János Lonovics Annamária Szabolcs Réka Sári András Tóth Julius G Papp András Varró Mária K Kovács Mike A Gray Barry E Argent Zsolt Boldogki | 2005 | World Journal of Gastroenterology2005，11，38： | 0 |
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