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| 1 | Quantification of sibutramine and its two metabolites in human plasma by LC-ESI-MS/MS and its application in a bioequivalence study显示文摘Obesity can be considered as a chronic illness of epidemic proportion and its incidents have increased exponentially in recent years. The use of anti-obesity drugs such as sibutramine is somewhat helpful. There is a need to quantify such drugs in biological samples, which is generally quite difficult. In this report, we developed and validated a simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of sibutramine (SB) and its two metabolites N-des methyl sibutramine (DSB) and N-di desmethyl sibutramine (DDSB) in human plasma. Zorbax SBC18 (4.6 mm 75 mm, 3.5 mm, 80 A) analytical column and 5 mM ammonium formate:acetonitrile (10:90, v/v) mobile phase were used for chromatographic separation of SB, DSB and DDSB. Multiple reaction monitoring (MRM) in the positive mode was used to detect SB, DSB and DDSB at m/z 280.3/124.9, 266.3/ 125.3 and 252.2/124.9, respectively. Liquid-liquid extraction was used for the extraction of analytes and internal standard from human plasma. This method was validated over a linear concentration range of 10.0-10,000.0 pg/mL for SB, DSB and DDSB with correlation coefficients (r) of Z0.9997. The drug and the two metabolites were stable in plasma samples. The validated method was successfully applied in a bioequivalence and pharmacokinetic study with human volunteers under fasting condition. | Venkata Suresh Ponnuru B.R.Challa RamaRao Nadendla | 2012 | Journal of Pharmaceutical Analysis2012，2，4： | 6 |
| 2 | Quantification of desloratadine in human plasma by LC-ESI-MS/MS and application to a pharmacokinetic study显示文摘A simple, sensitive, and specific liquid chromatography tandem mass spectrometry (LC-MS/ MS) method was developed for the quantification of desloratadine (DL) in human plasma using desloratadine-d5 (DLD5) as an internal standard (IS). Chromatographic separation was performed using an Xbridge C18 column (50 mm 4.6 mm, 5 mm) with an isocratic mobile phase composed of 10 mM ammonium formate: methanol (20:80, v/v), at a flow rate of 0.7 mL/min. DL and DLD5 were detected with proton adducts at m/z 311.2-259.2 and 316.2-264.3 in multiple reaction monitoring (MRM) positive modes, respectively. Liquid-liquid extraction (LLE) method was used to extract the drug and the IS. The method was validated over a linear concentration range of 5.0-5000.0 pg/mL with a correlation coefficient of (r2)Z0.9994. This method demonstrated intra- and inter-day precision within 0.7-2.0% and 0.7-2.7%, and an accuracy within 101.4-102.4%, and 99.5-104.8%. DL was found to be stable throughout the freeze-thaw cycles, bench-top, and postoperative stability studies. This method was successfully applied in the analysis of plasma samples following oral administration of DL (5 mg) in 35 healthy Indian male human volunteers under fasting conditions. | Venkata Suresh Ponnuru B.R. Challa Ramarao Nadendla | 2012 | Journal of Pharmaceutical Analysis2012，2，3： | 5 |
| 3 | Epigenetic field defects in progression to cancer显示文摘A field defect is a field of pre-malignant tissue in which a new cancer is likely to arise. Field defects often appear to be histologically normal under the microscope. Recent research indicates that cells within a field defect characteristically have an increased frequency of epigenetic alterations and these may be fundamentally important as underlying factors in progression to cancer. However, understanding of epigenetic field defects is at an early stage, and the work of Katsurano et al published this year, is a key contribution to this field. One question examined by Katsurano et al was how early could the formation of an epigenetic field defect be de-tected in a mouse colitis model of tumorigenesis. They highlighted a number of measurable epigenetic altera-tions, detected very early in normal appearing tissue undergoing histologically invisible tumorigenesis. They also documented the increasing presence of the epigenetic alterations at successive times during progression to cancer. In this commentary, we offer a perspective on the changes they observed within a broader sequence of epigenetic events that occur in progressionto cancer. In particular, we highlight the likely central role of epigenetic deficiencies in DNA repair gene expression that arise during progression to cancer. | Carol Bernstein Valentine Nfonsam Anil Ramarao Prasad Harris Bernstein | 2013 | World Journal of Gastrointestinal Oncology2013，5，3： | 3 |
| 4 | Groundwater travel time uncertainty analysis using sensitivity derivatives显示文摘 | LaVenue M Andrews R W Ramarao B S | 1998 | Water Resources Research1998，25，7： | 2 |
| 5 | Ventricular repolarization components on the electrocardiogram显示文摘 | Gan-Xin Yan Ramarao S Lankipalli James F Burke Simone Musco Peter R Kowey | 2003 | Journal of the American College of Cardiology2003，，： | 2 |
| 6 | Diallyl disulfide and diallyl trisulfide in garlic as novel therapeutic agents to overcome drug resistance in breast cancer显示文摘Breast cancer is one of the leading causes of cancer-related deaths in women worldwide.It is a cancer that originates from the mammary ducts and involves mutations in multiple genes.Recently,the treatment of breast cancer has become increasingly challenging owing to the increase in tumor heterogeneity and aggressiveness,which gives rise to therapeutic resistance.Epidemiological,populationbased,and hospital-based case-control studies have demonstrated an association between high intake of certain Allium vegetables and a reduced risk in the development of breast cancer.Diallyl disulfide(DADS)and diallyl trisulfide(DATS)are the main allyl sulfur compounds present in garlic,and are known to exhibit anticancer activity as they interfere with breast cancer cell proliferation,tumor metastasis,and angiogenesis.The present review highlights multidrug resistance mechanisms and their signaling pathways in breast cancer.This review discusses the potential anticancer activities of DADS and DATS,with emphasis on drug resistance in triple-negative breast cancer(TNBC).Understanding the anticancer activities of DADS and DATS provides insights into their potential in targeting drug resistance mechanisms of TNBC,especially in clinical studies. | RamaRao Malla Rakshmitha Marni Anindita Chakraborty Mohammad Amjad Kamal | 2022 | Journal of Pharmaceutical Analysis2022，12，2： | 2 |
| 7 | sympathetic nervous system and experimental diabetes:role of adrenal medullary hot-mones显示文摘 | Kaul CL Ramarao P | 2001 | Prog Drug Res2001，57，： | 1 |
| 8 | Partial protective effect of rutin on multiple low dose streptozotocin-induced diabetes in mice显示文摘 | SRINIVASAN K KAUL C L RAMARAO P | 2005 | Indian J Pharm2005，37，5： | 1 |
| 9 | Regulatory requirements and ICH guidelines on carcinogenicity testing of pharmaceuticals : a review on current status显示文摘 | JENA G B KAUL C L RAMARAO P | 2005 | Indian J Pharm2005，37，4： | 1 |
| 10 | Animal models in type 2 diabetes research: An overview 显示文摘 | Srinivasan K Ramarao P | 2007 | Indian Journal of Medical Research2007，125，3： | 1 |
| 11 | Helicobacter pylori induces but survives the extraccllular release of oxygen radicals from professional phagocytes using its catalase activity 显示文摘 | Ramarao N Gray-Owen SD Meyer TF | 2000 | Mol Microbiol2000，38，1： | 1 |
| 12 | Dementia of Alzheimer's dis- ease and other neurodegenerative disorders - mcmantine, a new hope显示文摘 | Sonkusare SK Kaul CL Ramarao P | 2005 | Pharmacol Res2005，51，1： | 1 |
| 13 | Ventricular repolarization components on the electrocardiogram:cellular basis and clinical significance显示文摘 | GAN-XIN Y RAMARAO S JAMES F | 2003 | J Am Coll Cardiol2003，42，： | 1 |
| 14 | One-pot synthesis of fluorine containing 3-cyano/ethoxycarbony1-2- methyl-benzofurans显示文摘 | RamaRao G Venkat Reddy Maitraie D | 2004 | Tetrahedron2004，60，12： | 1 |
| 15 | Deinking of recycled pulps using column flotation: energy and environmental benefits显示文摘 | S Chaiarrekij H Dhindra B V Ramarao | 2000 | Resources conservation and recycling2000，28，： | 1 |
| 16 | Changes of protein oxidation,calpain and cytoskeletal proteins (alpha tubulin and pNF-H) levels in rat brain after nerve agent poisoning显示文摘 | RamaRao G Acharya JN Bhattacharya BK | 2011 | Toxicol Lett2011，203，3： | 1 |
| 17 | Dementia of Alzheimer's disease and other neurodegenerative disorders-memantine,a new hope显示文摘 | Sonkusare SK Kaul CL Ramarao P | 2005 | Pharmacological Research2005，51，： | 1 |
| 18 | Dementia of Alzheimer's disease and other neurodegenerative disorders memantine, a new hope 显示文摘 | Sonkusare SK Kaul CL Ramarao P | 2005 | PharmacolRes2005，51，1： | 1 |
| 19 | Interspecific hybridizationbetween Brassica carinata and Brassica rapa显示文摘 | Choudhary B Joshi P Ramarao S | 2000 | Plant Breeding2000，119，： | 1 |
| 20 | Animal models in type 2 diabetes re- search: an overview 显示文摘 | Sfinivasan K Ramarao P | 2007 | Indian J Med Res2007，125，3： | 1 |
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