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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Exploratory study on microRNA profiles from plasma-derived extracellular vesicles in Alzheimer’s disease and dementia with Lewy bodies显示文摘Background:Because of the increasing life expectancy in our society,aging-related neurodegenerative disorders are one of the main issues in global health.Most of these diseases are characterized by the deposition of misfolded proteins and a progressive cognitive decline.Among these diseases,Alzheimer’s disease(AD)and dementia with Lewy bodies(DLB)are the most common types of degenerative dementia.Although both show specific features,an important neuropathological and clinical overlap between them hampers their correct diagnosis.In this work,we identified molecular biomarkers aiming to improve the misdiagnosis between both diseases.Methods:Plasma extracellular vesicles(EVs)-from DLB,AD and healthy controls-were isolated using size-exclusion chromatography(SEC)and characterized by flow cytometry,Nanoparticle Tracking Analysis(NTA)and cryo-electron microscopy.Next Generation Sequencing(NGS)and related bibliographic search was performed and a selected group of EV-associated microRNAs(miRNAs)was analysed by qPCR.Results:Results uncovered two miRNAs(hsa-miR-451a and hsa-miR-21-5p)significantly down-regulated in AD samples respect to DLB patients,and a set of four miRNAs(hsa-miR-23a-3p,hsa-miR-126-3p,hsa-let-7i-5p,and hsamiR-151a-3p)significantly decreased in AD respect to controls.The two miRNAs showing decreased expression in AD in comparison to DLB provided area under the curve(AUC)values of 0.9 in ROC curve analysis,thus suggesting their possible use as biomarkers to discriminate between both diseases.Target gene analysis of these miRNAs using prediction online tools showed accumulation of phosphorylation enzymes,presence of proteasome-related proteins and genes involved in cell death among others.Conclusion:Our data suggest that plasma-EV associated miRNAs may reflect a differential profile for a given dementia-related disorder which,once validated in larger cohorts of patients,could help to improve the differential diagnosis of DLB versus AD. | Ana Gámez-Valero Jaume Campdelacreu Dolores Vilas Lourdes Ispierto Ramón Reñé RamiroÁlvarez MPilar Armengol Francesc E.Borràs Katrin Beyer | 2019 | Translational Neurodegeneration2019，8，1： | 4 |
| 3 | MicroRNA expression and function in cancer显示文摘 | Ramiro G Muller F | 2006 | Trends in Molecular Medicine2006，12，12： | 1 |
| 4 | MicroRNAs prevent the generation of autoreactive antibodies 显示文摘 | Belver L de Y6benes V G Ramiro A R | 2010 | Immunity2010，33，5： | 1 |
| 5 | Immiscible poly- mer blends of semicrystalline bioeompatible components: Thermal properties and phase morphology analysis of PL- LA/PCL blends显示文摘 | Ramiro D Gabriel G Giovanni M | 2001 | Polymer2001，42，18： | 1 |
| 6 | Control of hypertension with medication: a comparative analysis of national surveys in 20 countries 显示文摘 | Nayu I David S Ramiro G | 2014 | Bull World Health Organ2014，92，1： | 1 |
| 7 | Influence of regeneration temperature on the phenols adsorption on activated carbon 显示文摘 | Sabio E Martin M L G Ramiro A | 2001 | Colloid Interface Sci2001，，242： | 1 |
| 8 | Influence of regeneration temperature on the phenols adsorption on activated carbon 显示文摘 | E Sabio M L G Martin A Ramiro | 2001 | Colloid Interface Science2001，24，2： | 1 |
| 9 | Timing of antibiotic prophylaxis {or primary total knee arthroplasty per{ormed during ischemia显示文摘 | Soriano A Bori G Garcia- Ramiro S | 2008 | Prophylaxis for Knee Arthroplasty2008，46，1： | 1 |
| 10 | Development of hybrid polymeric materials based on thiol-ene/cationic formulations显示文摘 | RICARDO A O BERTHA A PUENTE URBINA RAMIRO G S | | 0，，： | 1 |
| 11 | Immiscible polymer blends of semicrystalline bioco mpatible components: thermal properties and phase morphology analysis of PLA/PCL blends 显示文摘 | RAMIRO D GABRIEL G GIOVANNI M | 2001 | Polymer2001，42，18： | 1 |
| 12 | Influence of regeneration temperature on the phenols adsorption on acti- vated carbon显示文摘 | SABIO E MATIN ML G RAMIRO A | 2001 | Colloid Interface Sci2001，242，： | 1 |
| 13 | Influence of regeneration temperature on the phenols adsorption on activated carbon显示文摘 | E Sabio M L G Martin A Ramiro | | 0，，02： | 1 |
| 14 | Immiscible polymer blends of semicrystalline biocompatible components:Thermal properties and phase morphology analysis of PLLA/PCL blends显示文摘 | Ramiro D E Gabiel G Giovanni M | 2001 | Polymer2001，42，17： | 1 |
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