|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis显示文摘Iron(Fe)deficiency is prevalent in plants grown in neutral or alkaline soil.Plants have evolved sophisticated mechanisms that regulate Fe homeostasis,ensuring survival.In Arabidopsis,FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a crucial regulator of Fe-deficiency response.FIT is activated indirectly by basic helix-loop-helix(bHLH)IVc transcription factors(TFs)under Fed eficiency;how ever,it remains unclear which protein(s)act as the linker to mediate the activation of FIT by bHLH IVc TFs.In this study,we characterize the functions of bHLH121 and demonstrate that it directly associates with the FIT promoter.We found that loss-of-function mutations of bHLH121 cause severe Fedeficiency symptoms,reduced Feaccum ulation,and disrupted expression of genes associated with Fehomeostasis.Genetic analysis showed that FIT is epistatic to bHLH121 and FIT overexpression partially rescues the bhlh121 mutant.Further investigations revealed that bHLH IVc TFs interact with and promote nuclear accumulation of bHLH121.We demonstrated that bHLH121 has DNA-binding activity and can bind the prom oters of the FIT and bHLHlb genes,but we did not find that it has either direct transcriptional activation or repression activity tow ard these genes.Meanw hile,we found that bHLH121 functions downstream of and is a direct target of bHLH IVc TFs,and its expression is induced by Fe deficiency in a bHLH IV c-dependent manner.Taken together,these results establish that bHLH121 functions together with bHLH IVc TFs to positively regulate the expression of FIT and thus plays a pivotal role in maintaining Fe homeostasis in Arabidopsis. | Rihua Lei Yang Li Yuerong Cai Chenyang Li Mengna Pu Chengkai Lu Yujie Yang Gang Liang | 2020 | Molecular Plant2020，13，4： | 14 |
| 2 | A sulfur dioxide polymer prodrug showing combined effect with doxorubicin in combating subcutaneous and metastatic melanoma显示文摘Melanoma,as the most aggressive and treatment-resistant skin malignancy,is responsible for about 80%of all skin cancer mortalities.Prone to invade into the dermis and form distant metastases significantly reduce the patient survival rate.Therefore,early treatment of the melanoma in situ or timely blocking the deterioration of metastases is critical.In this study,a sulfur dioxide(SO_(2))polymer prodrug was designed as both an intracellular glutathione(GSH)-responsive SO_(2) generator and a carrier of doxorubicin(DOX),and used for the treatment of subcutaneous and metastatic melanoma.Firstly,chemical conjugation of 4-N-(2,4-dinitrobenzenesulfonyl)-imino-1-butyric acid(DIBA)onto the side chains of methoxy poly(ethylene glycol)grafted dextran(mPEG-g-Dex)resulted in the synthesis of the amphiphilic polymer prodrug of SO_(2),mPEG-g-Dex(DIBA).The obtained mPEG-g-Dex(DIBA)could self-assemble into stable micellar nanoparticles and exhibited a glutathione-responsive SO_(2) release behavior.Subsequently,DOX was encapsulated into the core of mPEG-g-Dex(DIBA)micelles to form DOX-loaded nanoparticles(PDDN-DOX).The formed PDDN-DOX could be internalized by B16F10 cells and synchronously release DOX and SO_(2) into the tumor cells.As a result,PDDN-DOX exerted synergistic anti-tumor effects in B16F10 melanoma cells because of the oxidative damage properties of SO_(2) and toxic effects of DOX.Furthermore,in vivo experiments verified that PDDN-DOX had great potential for the treatment of subcutaneous and metastasis melanoma.Collectively,our present work demonstrates that the combination of SO_(2)-based gas therapy and chemotherapeutics offers a new avenue for inhibiting melanoma progression and metastases. | Lin An Peng Zhang Wei Shen Xuan Yi Weitian Yin Rihua Jiang Chunsheng Xiao | 2021 | Bioactive Materials2021，6，5： | 3 |
| 3 | Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01显示文摘 | Qian Shen Bowen Zhang Rihua Xu Yang Wang Xuelong Ding Pinglan Li | 2010 | Anaerobe2010，，4： | 3 |
| 4 | Comparative proteomic analysis of the effects of high-concentrate diet on the hepatic metabolism and inflammatory response in lactating dairy goats显示文摘Background: To understand the impact of feeding a high-concentrate diet to mid-lactating goats for a long time on liver metabolism and inflammatory response, two dimensional polyacrylamide gel electrophoresis(2-DE) and real-time PCR method were employed to detect proteins differentially expressed in liver and their m RNAs expression in goats fed high concentrate diet(HC) or low concentrate diet(LC). Twelve lactating dairy goats were randomly assigned to either a HC diet group(65 % concentrate of dry matter; n = 6) or a LC diet group(35 % concentrate of dry matter; n = 6) for 10 wk.Results: Twenty differentially expressed spots(≥2.0-fold changes) in the hepatic tissues were excised and successfully identified using MALDI TOF/TOF. Of these, 8 proteins were up-regulated, while the rest 12 proteins were down-regulated in HC goats compared to LC. Differential expressed proteins including alpha enolase 1(ENO1), glutamate dehydrogenase 1(GLUD1), glutathione S-transferase A1(GSTA1), ATP synthase subunit 5β(ATP5β), superoxide dismutase [Cu-Zn](SOD1), cytochrom c oxidase subunit Via(COX6A1) and heat shock protein 60(HSP60) were further verified by real-time PCR and/or western blot at m RNA or protein expression level. Consistent with the 2-DE results, a significant decrease of β-actin protein expression and SOD enzyme activity was observed in liver of HC goats(P < 0.05), while ENO1 protein expression was significantly up-regulated in HC compared to LC goats(P < 0.05). However, western blot analysis did not show a significant difference of hepatic HSP60 protein between HC and LC group, which did not match the decrease of HSP60 content detected by 2-DE analysis. Real-time PCR showed that glutathione S-transferase P1(GSTP1) and SOD1 m RNA expression was significantly decreased in liver of HC goats, while cytochrom c oxidase(COX3) and ATPase 8(ATP8) m RNAs expression were markedly increased compared to LC(P < 0.05). Gene Ontology(GO) analysis revealed that HC diet resulted in altered expression of proteins related to catalytic and mitochondrial metabolism in the liver, and may increase the stress response with up-regulating the expression of differentiation 14(CD14) cluster and serum amyloid A(SAA) as well as C-reactive protein(CRP) in the liver.Conclusions: These results suggest that feeding high concentrate diet to lactating goats for 10 wk leads to the activation of the inflammatory response, and decreases the anti-oxidant capacity, and subsequently impairs the mitochondrial function in the liver. | Yongqian Duanmu Rihua Cong Shiyu Tao Jing Tian Haibo Dong Yuanshu Zhang Yingdong Ni Ruqian Zhao | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 2 |
| 5 | Quantitative trait loci for cold tolerance of rice recombinant inbred lines in low temperature environments显示文摘 | Wenzhu Jiang Yong-Mei Jin Joohyun Lee Kang-Ie Lee Rihua Piao Longzhi Han Jin-Chul Shin Rong-De Jin Tiehua Cao Hong-Yu Pan Xinglin Du Hee-Jong Koh | 2011 | Molecules and Cells2011，，6： | 2 |
| 6 | Robust Lane Detection and Tracking Based on Machine Vision显示文摘Lane detection based on machine vision,a key application in intelligent transportation,is generally characterized by gradient information of lane edge and plays an important role in advanced driver assistance systems(ADAS).However,gradient information varies with illumination changes.In the complex scenes of urban roads,highlight and shadow have effects on the detection,and non-lane objects also lead to false positives.In order to improve the accuracy of detection and meet the robustness requirement,this paper proposes a method of using top-hat transformation to enhance the contrast and filter out the interference of non-lane objects.And then the threshold segmentation algorithm based on local statistical information and Hough transform algorithm with polar angle and distance constraint are used for lane fitting.Finally,Kalman filter is used to correct lane lines which are wrong detected or missed.The experimental results show that computation times meet the real-time requirements,and the overall detection rate of the proposed method is 95.63%. | FAN Guotian LI Bo HAN Qin JIAO Rihua QU Gang | 2020 | ZTE Communications2020，18，4： | 2 |
| 7 | Growth and Luminescence Characteristics of Undoped LaCl3 Crystal by Modified Bridgman Method 显示文摘 | Yu P Xiaofeng C Rihua M | 2005 | Journal of Crystal Growth2005，279，： | 1 |
| 8 | New types of lead tungstate crystals with high light yield 显示文摘 | MAO Rihua QU Xiangdong REN Guohao | 2002 | Nucl Instrum Methods A2002，486，： | 1 |
| 9 | Large size LY- SO-LSO crystals for future high energy physics experiments 显示文摘 | Chen Jianming Mao Rihua Zhang Liyuan | 2007 | IEEE Trans Nuel Sci2007，54，： | 1 |
| 10 | Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01显示文摘 | Qian Shen Bowen Zhang Rihua Xu Yang Wang Xuelong Ding Pinglan Li | 2010 | Anaerobe2010，，4： | 1 |
| 11 | Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis01显示文摘 | SHEN Qian ZHANG Bowen XU Rihua | 2010 | Anaerobe2010，16，4： | 1 |
| 12 | New types of lead tungstate crystals with high light yield 显示文摘 | MAO Rihua QU Xiangdong REN Guohao | 2002 | Nucl Instrum Methods2002，486，： | 1 |
| 13 | In vitro and in vivo antioxidant activity of exopolysaccharide fractions from Bifidobacterium animalis RH显示文摘 | Rihua Xu Nan Shang Pinglan Li | 2011 | Anaerobe2011，，5： | 1 |
| 14 | 《心住南海》英文节选(续)(英文)显示文摘After accomplishing his mission of recovering the Yongxing Island,Li Jing Sen returned to Guangzhou on the naval ship Yong Xing.The beautiful scenes of the blue ocean,lush vegetation on the island,and flocks of seabirds kept appearing in his mind.The crew’s next assignment was | Pan Jiansheng Zhuo Rihua | 2014 | 中国远洋航务2014，0，12： | 0 |
| 15 | Development of metal-recycling technology in waste crystalline-silicon solar cells显示文摘Solar energy is currently one of the most promising clean energy sources and the use of solar energy has led to a rapid increase in the number of solar cells.As one of the fastest-growing electronic wastes,the resource treatment of solar cells at the end of their life should not be neglected.This review discusses the trend for the market development of crystalline-silicon solar cells and analyzes their physical structure and composition.It also discusses the current domestic and international recycling technologies for crystalline-silicon solar cells,including manual dismantling,inorganic acid dissolution,the combination of heat-treatment and chemical methods,and organic solvent dissolution.The shortcomings of the above treatment methods are discussed and some views on the recycling of waste crystalline-silicon solar cells are presented.Constructive suggestions for the green and sustainable development of crystalline-silicon solar cells are put forward by comparing different treatment-recycling processes. | Dehai Lin Zilin Liu Xiaoduan Li Zixiong Cao Rihua Xiong | 2023 | Clean Energy2023，7，3： | 0 |
| 16 | Experimental Study of Rotor Flow Separation Control using a New Type of Dielectric Barrier Discharge Plasma Actuator显示文摘Flow separation occurring on rotor blades is an important limiting factor for helicopter performance. This paper presents a new type of dielectric barrier discharge(DBD) plasma actuator for rotor blade flow separation control called bipolar DBD plasma actuator. The bipolar DBD plasma actuator's connection mode differs from traditional plasma actuators and eliminates reverse discharge between electrodes. The experiments in this work were carried out by smoke-wire flow visualization and PIV technology in the open test section of a low speed wind tunnel, and solved the problem of high voltage electricity supplied to the plasma actuator while the rotor rotated. In this experiment, the rotor, camera, and laser were synchronized to obtain results. Smoke-wire results and PIV results illustrated that the flow separation weakened with increasing rotor speed; the separated flow area was large at a rotating speed of 300 r/min and gradually became smaller at the rotating speed of 600 r/min, the flow separation disappeared at the rotating speed of 1200 r/min. When the plasma was active, both smoke-wire results and PIV results showed the flow separation area was greatly reduced at the rotating speed of 300 r/min and disappeared at the rotating speeds of 600 r/min and 1200 r/min, the rotor flow separation at high angles of attack could be effectively controlled by the new DBD plasma actuator. | HU Xu GAO Chao HAO Jiangnan ZHANG Zhengke XUE Ming YAN Rihua | 2019 | Journal of Thermal Science2019，28，2： | 0 |
| 17 | Association between change of health care providers and pregnancy exposure to FDA category C, D and X drugs显示文摘 | Yang Jianzhou Xie Rihua Daniel Krewski Wang Yongjin Mark Walker Cao Wenjun Shi Wu Wen | 2014 | Chinese Medical Journal2014，，4： | 0 |
| 18 | 《心住南海》英文节选(续)(英文)显示文摘(continued from last issue)Today’s Xi Sha On July 17,2012,the Sansha City People’s Congress preparation committee was set up.The city would cover an island area of 13 square k ilometers and sea area of over 2 million square kilometers.A week later,Sansha formally became a city of Hainan Province.The Xinhua News Agency reported:'The setup of Sanshaas a prefectural level cityis an adjustment and improvement to our administrative system in our Xi Sha,Zhong Sha and Nan Sha Islands | Pan Jiansheng Zhuo Rihua | 2015 | 中国远洋航务2015，0，2： | 0 |
| 19 | Brazilein intervention for sciatic nerve injury in BALB/c mice显示文摘Brazilein has been shown to exert an immune regulation effect on regeneration in the central nervous system,but there is currently no consensus regarding its effect on sciatic nerve injury.In the current study,western blot and real-time PCR detection revealed that,after 16 and 8 g/kg of brazilein intervention,the amount of S100 protein and mRNA at L4-6 spinal segments of sciatic nerve injured mice was significantly greater than after 4 g/kg brazilein administration,and in an untreated model group.Luxol Fast Blue myelin staining revealed that neural regeneration after 16 and 8 g/kg of brazilein intervention was significantly better than after 4 g/kg brazilein administration,and in the model group.In addition,electrophysiological and immunohistochemical examinations further confirmed the effect of brazilein in repairing sciatic nerve injury in BALB/c mice.These results indicated that brazilein was able to activate the S100 in anterior horn cells of the spinal cord,promoting sciatic nerve regeneration and repair.Both moderate and high doses were found to exert significant effects. | Zhan Zhang Jian Cao Weitian Yin Lisen Li Yuxi Jia Haoyu Liu Mingming Zhao Rihua Jiang | 2011 | Neural Regeneration Research2011，6，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员