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| 1 | Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro显示文摘Amelogenin(AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG(rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts(OCCM-30)were treated with different concentrations(0.1, 1, 10, 100, 1 000, 10 000, 100 000 ng · mL^(-1)) of recombinant human AMG(rhAMG)and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein(BSP), osteocalcin(OCN), collagen type I(COL I), osteopontin(OPN), runt-related transcription factor 2(Runx2), cementum attachment protein(CAP), and alkaline phosphatase(ALP) genes using quantitative RT-PCR. The dose response of rhAMG was evaluated using a real-time cell analyzer.Total RNA was isolated on day 3, and cell mineralization was assessed using von Kossa staining on day 8. COL I, OPN and lysosomalassociated membrane protein-1(LAMP-1), which is a cell surface binding site for amelogenin, were evaluated using immunocytochemistry. F-actin bundles were imaged using confocal microscopy. rhAMG at a concentration of 100 000 ng · mL^(-1) increased cell proliferation after 72 h compared to the other concentrations and the untreated control group. rhAMG(100 000 ng · mL^(-1)) upregulated BSP and OCN mRNA expression levels eightfold and fivefold, respectively. rhAMG at a concentration of 100 000 ng · mL^(-1) remarkably enhanced LAMP-1 staining in cementoblasts. Increased numbers of mineralized nodules were observed at concentrations of 10 000 and 100 000 ng · mL^(-1) rhAMG. The present data suggest that rhAMG is a potent regulator of gene expression in cementoblasts and support the potential application of rhAMG in therapies aimed at fast regeneration of damaged periodontal tissue. | Sema S.Hakki S.Buket Bozkurt Emre Türkay Michel Dard Nuhan Purali Werner Gotz | 2018 | International Journal of Oral Science2018，10，2： | 3 |
| 2 | Effect of Angiotensin-converting Enzyme Inhibition on Experimental Hepatic Fibrogenesis显示文摘 | Cansel Türkay ?zlem Y?nem Sema Ar?c? Ayhan Koyuncu Mehmet Kanbay | 2008 | Digestive Diseases and Sciences2008，，3： | 1 |
| 3 | A novel approach for assessment of candidate technologies with respect to their innovation potentials: Quick innovation intelligence process显示文摘 | Türkay Dereli Koray Altun | 2013 | Expert Systems With Applications2013，，3： | 1 |
| 4 | Logic-based MINLP algorithm for the optimal synthesis of process networks显示文摘 | Türkay M Grossmann I E | 1996 | Computer and Chemical Engineering1996，20，8： | 1 |
| 5 | Pediatric sinusitis:when do we operate显示文摘 | Lieser JD De Rkay CS | | 0，，1： | 1 |
| 6 | IGF-I levels and visceral fat accumulation in colonic neoplasia显示文摘 | ERARSLAN E CO?KUN Y T?RKAY C | 2014 | Clin Res Hepatol Gastroenterol2014，38，1： | 1 |
| 7 | Formulation and implementation of parametric optimisation of a washing machine suspension system显示文摘 | O.S. Türkay B. Kiray A.K. Tugcu ?.T. Sümer | 1995 | Mechanical Systems and Signal Processing1995，，4： | 1 |
| 8 | Influence of rheological additives on char formation and fire resistance of intumes-cent coatings显示文摘 | B Bodzay K Bocz Zs Brkai | 2011 | Polymer Degradation and Stability2011，96，3： | 1 |
| 9 | Multicompany collaborative supply chain management with economical and environmental considerations 显示文摘 | Türkay M Oruc C Fujita K Asakura T | 2004 | Computers and Chemical Engineering2004，28，： | 1 |
| 10 | Helicobacter pylori and histopathological findings in patients with dyspepsia显示文摘 | Türkay C Erbayrak M Bavbek N | 2011 | Turk J Gastroenterol2011，22，2： | 1 |
| 11 | Revision of the Helice/Chasmagnathus complex (Crustacea:Decapoda:Brachyura)显示文摘 | Sakai K Türkay M Yang S L | 2006 | Abhandlungen der Senckenbergischen Naturforschenden Gesellschafl2006，565，： | 1 |
| 12 | 海南水域玉蟹科六新种记述(甲壳动物 :十足目 :短尾次目 )(英文)显示文摘海南水域蟹类区系十分丰富 ,仅玉蟹科就有 54种 ,本文报道了其中发现 6新种。1 秀丽异核果蟹 ,新种Heteronuciaeleganssp .nov .新种与球形异核果蟹H .globataSakai,1963很近似 ,但新种的头胸甲突起均匀分布 ,不具成群突起 ,靠近前侧缘的突起呈长圆锥形 ,额叶平直 ,后缘也平直等特征与后者不同。2 疣异核果蟹 ,新种Heteronuciatuberculatasp .nov .新种与雅异核果蟹H .venusta很近似 ,但新种头胸甲表面具长圆锥形突起 ,边缘的突起较大 ,表面的突起更密集 ,额叶边缘在外角处具一较大的齿 ,近中央缺刻处具一个较小的齿 ,头胸甲后缘具一排大而密集的突起 ,螯掌的上表面具 3枚粗大的圆锥形突起 ,第 3颚足外肢表面除了颗粒外还有一排 3枚粗大突起等特征与后者不同。3 新村异核果蟹 ,新种Heteronuciaxincunensissp .nov .新种与奥氏异核果蟹H .oeschiWard ,194 1很近似 ,但新种的头胸甲胃区每边有 2枚圆形隆起 ,额缘的外角呈圆形等特征与后者不同。4 海南长臂蟹 ,新种Myrahainanicasp .nov .新种与近亲长臂蟹M .affinisBell,1855很近似 ,但新种的头胸甲后缘的侧齿呈直角 ,雄性后缘中刺的长度为其基部宽度的 2倍 ,雄性螯足长节表面末 1/3处有小颗粒 ,掌长为宽的 2倍 ,表面具稀疏颗粒和硬毛 ,第3颚足外肢末? | 陈惠莲 Michael Tǖrkay | 2001 | 动物分类学报2001，26，3： | 0 |
| 13 | A NEW SPECIES OF RANINOIDES (CRUSTACEA: DECAPODA: RANINIDAE) FROM OFF THE SOUTHEASTERN COAST OF HAINAN ISLAND AND NANSHA ISLANDS显示文摘Raninoides longifrons new species, off SE coast of Hainan Island, dorsal surface of carapace with an arched granular edge across carapace between the anterolateral teeth, median frontal tooth longest, second frontal teeth subquadrate. | 陈惠莲 Michael Türkay | 2001 | Chinese Journal of Oceanology and Limnology2001，19，4： | 0 |
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