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| 1 | Dynamic chromatin states in human ES cells reveal potential regulatory sequences and genes involved in pluripotency显示文摘Pluripotency,一个细胞的能力区分并且产生所有胚胎的系,定义象胚胎的茎(ES ) 那样的哺乳动物的细胞类型的一个小数字细胞。当它通常被保持了时,那 pluripotency 是 transcriptional 的产品激活并且维持关键干细胞基因的表达式的规章的网络,积累的证据在建立并且保卫 ES 房间的 pluripotency 为 epigenetic 进程正在指向一个关键角色,象维持区分的房间类型的身份一样。以便更好在 pluripotency 理解 epigenetic 机制的角色,我们检验了在经历区别进一个 mesendodermal 系的人的 ES 房间(hESCs ) 染色体宽的染色质修正的动力学。我们发现在倡导者的染色质修正在区别期间仍然保持大部分不变,除了在在在 H3K27 的 acetylation 和 methylation 之间的一个动态开关标记在基因表示的激活和 silencing 之间的转变的倡导者的一个小数字,建议在在大多数差别上的房间命运承诺的一个层次表示了基因。我们也在 50 000 潜在的 enhancers 上印射,并且在染色质修正,特别 H3K4me1 和 H3K27ac 观察了大得多的动力学,它与他们的潜在的目标基因的表示相关。这些 enhancers 的进一步的分析揭示了 pluripotency 和可以在 hESCs 授与发展胜任的一个平衡状态的一口染色质签名陈述语气的潜在地关键的 transcriptional 管理者。我们的结果提供在定义 enhancers 和 pluripotency 支持染色质修正的角色的新证据。 | R David Hawkins Gary C Hon Chuhu Yang Jessica E Antosiewicz-Bourget Leonard K Lee Que-Minh Ngo Sarit Klugman Keith A Ching Lee E Edsall Zhen Ye Samantha Kuan Pengzhi Yu Hui Liu Xinmin Zhang Roland D Green Victor V Lobanenkov Ron Stewart James A Thomson Bing Ren | 2011 | Cell Research2011，21，10： | 6 |
| 2 | Upper esophageal sphincter abnormalities are strongly predictive of treatment response in patients with achalasia显示文摘AIM: To investigate the relationship between upper esophageal sphincter abnormalities achalasia treatment METHODS: We performed a retrospective study of 41 consecutive patients referred for high resolution esophageal manometry with a final manometric diagnosis of achalasia. Patients were sub-divided by presence or absence of Upper esophageal sphincter(UES) abnormality, and clinical and manometric profiles were compared.Correlation between UES abnormality and sub-type(i.e.,hypertensive, hypotensive or impaired relaxation) and a number of variables, including qualitative treatment response, achalasia sub-type, co-morbid medical illness,psychiatric illness, surgical history, dominant presentingsymptom, treatment type, age and gender were also evaluated.RESULTS: Among all 41 patients, 24(58.54%) had a UES abnormality present. There were no significant differences between the groups in terms of age, gender or any other clinical or demographic profiles. Among those with UES abnormalities, the majority were either hypertensive(41.67%) or had impaired relaxation(37.5%) as compared to hypotensive(20.83%), although this did not reach statistical significance(P = 0.42). There was no specific association between treatment response and treatment type received; however, there was a significant association between UES abnormalities and treatment response. In patients with achalasia and concomitant UES abnormalities, 87.5% had poor treatment response, while only 12.5% had favorable response. In contrast, in patients with achalasia and no UES abnormalities, the majority(78.57%) had good treatment response, as compared to 21.43% with poor treatment response(P = 0.0001). After controlling for achalasia sub-type, those with UES abnormality had 26 times greater odds of poor treatment response than those with no UES abnormality(P = 0.009). Similarly, after controlling for treatment type, those with UES abnormality had 13.9 times greater odds of poor treatment response compared to those with no UES abnormality(P = 0.017).CONCLUSION: The presence of UES abnormalities in patients with achalasia significantly predicted poorer treatment response as compared to those with normal UES function. | Simon C Mathews Maria Ciarleglio Yamile Haito Chavez John O Clarke Ellen Stein Bani Chander Roland | 2014 | World Journal of Clinical Cases2014，2，9： | 5 |
| 3 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 4 | Acceleration schemes with application to the EM algorithm显示文摘 | BERLINET A ROLAND C | 2007 | Computational Statistics and Data Analysis2007，51，： | 1 |
| 5 | ICAM-1 expression deter- mines malignant potential of cancer 显示文摘 | Roland C L Harken A H Sarr M G | 2007 | Surgery2007，141，6： | 1 |
| 6 | Aging of the secondary relaxation to probe structural relaxation in the glassy state 显示文摘 | Casalini R Roland C M | 2009 | Physical Review Letters2009，102，03： | 1 |
| 7 | P-nitrophenol-α-glucopyramoside as substrate for measurement of maltase activity in human semen显示文摘 | Pierre C Roland R Tremblay D | 1978 | J Clin Chem1978，24，2： | 1 |
| 8 | Neural network-based combustion optimization reduces NOX emissions while improving performance显示文摘 | R C ROLAND | 1998 | Booth Dynamic Modeling Control Applications for Industry Workshop1998，30，1： | 1 |
| 9 | The Ability of Stroke Volume Variations Obtained with Vigileo/FloTrac System to Monitor Fluid Responsiveness in Mechanically Ventilated Patients显示文摘 | Maxime Cannesson Henri Musard Olivier Desebbe Cécile Boucau Rémi Simon Roland Hénaine Jean-Jacques Lehot | 2009 | Anesthesia & Analgesia2009，，2： | 1 |
| 10 | Models for the component dynamics in blends and mixtures显示文摘 | NGAI K L ROLAND C M | 2004 | Molecular Crystals and Liquid Crystals2004，77，3： | 1 |
| 11 | Analytical modelfor a geomorphological instantaneous unit hydrograph显示文摘 | FLEURANT C KARTIWA B ROLAND B | 2006 | Hydrological Processes2006，20，： | 1 |
| 12 | Clinical significance of fever after percutaneous nephrolithotomy显示文摘 | JEFFREY A C ROLAND C JAY B | 1998 | Urology1998，52，1： | 1 |
| 13 | Pressure evolution of the excess wing in a l'ype-B glass former 显示文摘 | Casalini R Roland C M | 2003 | Physical Review Letters2003，91，01： | 1 |
| 14 | Histroy and principles of Shack-Hartmann wavefront sensing显示文摘 | Ben C Platt Roland Shack | 2001 | J Refract Surg2001，17，5： | 1 |
| 15 | Asorbate and dehydroaseorbate influence cell cycle progression in a tobacco cell suspension显示文摘 | Potters G Nele H Roland J C | 2000 | Plant Physiol2000，11，24： | 1 |
| 16 | The bulk modulus and Poisson's ratio of' incompressible' materials 显示文摘 | Mott P H Dorganl J R Roland C M | 2008 | Journal of Sound and Vibration2008，312，45： | 1 |
| 17 | Stability analysis of passive dynamic walking of quadrupeds显示文摘 | David C Remy Keith Buffinton Roland Siegwart | 2010 | International Journal of Robotics Research2010，，29： | 1 |
| 18 | On image analysis by the method of moments显示文摘 | Roland C H Chin T | 1998 | IEEE Transactions on Pattern Analysis and Machine Intelligence1998，10，4： | 1 |
| 19 | Development of a PCR based assay for rapid and reliable identification of pathogenic Fusarium显示文摘 | Prashant K M Roland T V Alastair C | 2003 | FEMS Microbiology Letters2003，218，2： | 1 |
| 20 | P-Nitrophenol - a -glucopyranoside as substrate for measurement of mahase activity in human semem显示文摘 | Pierre C Roland R Tremblay | 1978 | J Clin Chem1978，24，： | 1 |
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