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| 1 | Antergrade ejaculatory duct recanalization and dilation 显示文摘 | JONATHAN PJ RONALD JZ | 1995 | Urology1995，46，5： | 1 |
| 2 | Continued drug use and other cofactors for progression to AIDS among injecting drug users 显示文摘 | Ronald PJ Robertson JR Elton RA | 1994 | AIDS1994，8，3： | 1 |
| 3 | Thyroid dysfunction dur- ing pregnancy and in the first postpartum year in women with dia- betes mellitustype显示文摘 | Robert PJ Ronald PS Bakker K | 2002 | Eur J Endocrinol2002，47，： | 1 |
| 4 | The role of mdr2 Pglycoprotein in hepatobiliary lipid transport显示文摘 | Ronald PJ Oude E Guido NJ | 1997 | FASEB1997，11，： | 1 |
| 5 | Ephrin A2 receptor targeting does not increase adenoviral pancreatic cancer transduction in vivo显示文摘AIM:To generate an adenoviral vector specifically targeting the EphA2 receptor(EphA2R) highly expressed on pancreatic cancer cells in vivo.METHODS:YSA,a small peptide ligand that binds the EphA2R with high affinity,was inserted into the HI loop of the adenovirus serotype 5 fiber knob.To further increase the specificity of this vector,binding sites for native adenoviral receptors,the coxsackie and adenovirus receptor(CAR) and integrin,were ablated from the viral capsid.The ablated retargeted adenoviral vector was produced on 293T cells.Specifi c targeting of this novel adenoviral vector to pancreatic cancer was investigated on established human pancreatic cancer cell lines.Upon demonstrating specifi c in vitro targeting,in vivo targeting to subcutaneous growing human pancreatic cancer was tested by intravenous and intraperitoneal administration of the ablated adenoviral vector.RESULTS:Ablation of native cellular binding sites reduced adenoviral transduction at least 100-fold.Insertion of the YSA peptide in the HI loop restored adenoviral transduction of EphA2R-expressing cells but not of cells lacking this receptor.YSA-mediated transduction was inhibited by addition of synthetic YSA peptide.The transduction specificity of the ablated retargeted vector towards human pancreatic cancer cells was enhanced almost 10-fold in vitro.In a subsequent in vivo study in a nude(nu/nu) mouse model however,no increased adenoviral targeting to subcutaneously growing human pancreas cancer nodules was seen upon injection into the tail vein,nor upon injection into the peritoneum.CONCLUSION:Targeting the EphA2 receptor increases specificity of adenoviral transduction of human pancreatic cancer cells in vitro but fails to enhance pancreatic cancer transduction in vivo. | Michael A van Geer Conny T Bakker Naoya Koizumi Hiroyuki Mizuguchi John G Wesseling Ronald PJ Oude Elferink Piter J Bosma | 2009 | World Journal of Gastroenterology2009，15，22： | 1 |
| 6 | Thyroid dysfunction during pregnancy and i n the fist postpartum year in women with diabetes mellitus type1显示文摘 | Robert PJ Ronald PS | 2002 | Eur J endocrinol2002，147，8： | 1 |
| 7 | Thyroid dysfunction during pregnancy and in the first postpartum year in women with diabe- tes mellitus type 显示文摘 | Robert PJ Ronald PS Bakker K | 2002 | Eur J Endocrinol2002，147，4： | 1 |
| 8 | Ex-vivo evaluation of gene therapy vectors in human pancreatic (cancer) tissue slices显示文摘AIM:To culture human pancreatic tissue obtained from small resection specimens as a pre-clinical model for examining virus-host interactions. METHODS:Human pancreatic tissue samples (malignant and normal)were obtained from surgical specimens and processed immediately to tissue slices. Tissue slices were cultured ex vivo for 1-6 d in an incubator using 95%O2.Slices were subsequently analyzed for viability and morphology.In addition the slices were incubated with different viral vectors expressing the reporter genes GFP or DsRed. Expression of these reporter genes was measured at 72 h after infection.RESULTS:With the Krumdieck tissue slicer,uniform slices could be generated from pancreatic tissue but only upon embedding the tissue in 3%low melting agarose.Immunohistological examination showed the presence of all pancreatic cell types.Pancreatic normal and cancer tissue slices could be cultured for up to 6 d,while retaining viability and a moderate to good morphology.Reporter gene expression indicated that the slices could be infected and transduced efficiently by adenoviral vectors and by adeno associated viral vectors,whereas transduction with lentiviral vectors was limited.For the adenoviral vector,the transduction seemed limited to the peripheral layers of the explants. CONCLUSION:The presented system allows reproducible processing of minimal amounts of pancreatic tissue into slices uniform in size,suitable for pre-clinical evaluation of gene therapy vectors. | Michael A van Geer Koert FD Kuhlmann Conny T Bakker Fibo JW ten Kate Ronald PJ Oude Elferink Piter J Bosma | 2009 | World Journal of Gastroenterology2009，15，11： | 1 |
| 9 | Thyroid dysfunction during pregnancy and in the first postpartum year in woman with diabetes mcllitus Type 显示文摘 | Robert PJ Ronald PS Bakker K | 2002 | Eur J Endoerinol2002，47，： | 1 |
| 10 | Targeting cancer stem cells by inhibiting Wnt, Notch and Hedgehog pathways显示文摘 | Takebe N Harris PJ Ronald QW | 2010 | Nat RevClinOncol2010，8，2： | 1 |
| 11 | Thyroid dysfunction during pregnancy and in the first postpartum year in women with dia- betes mellitustype I显示文摘 | Robert PJ Ronald PS Bakker K | 2002 | Eur J Endocfinol2002，47，： | 1 |
| 12 | Thyroid dysfunction during pregnancy and in the fist postpartum year in women with diabetes mellitus type 1 显示文摘 | Robert PJ Ronald PS Bakker K | 2002 | Eur J endocrinol2002，147，8： | 1 |
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