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| 1 | Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection显示文摘Hepatitis delta virus(HDV) seems to strongly suppress hepatitis B virus(HBV)replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein(HBx) is essential for HBV replication, determination of HBV X gene(HBX)quasispecies complexity in HBV/HDV infection compared to HBV monoinfection may provide information on the interactions between these two viruses.AIM To compare HBV quasispecies complexity in the HBX 5' region between chronic hepatitis delta(CHD) and chronic HBV mono-infected patients.METHODS Twenty-four untreated patients were included: 7/24(29.2%) with HBeAgnegative chronic HBV infection(CI, previously termed inactive carriers), 8/24(33.3%) with HBeAg-negative chronic hepatitis B(CHB) and 9/24(37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels.The HBX 5' region [nucleotides(nt) 1255-1611] was then PCR-amplified for subsequent next-generation sequencing(MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidencebased indices(number of haplotypes and number of mutations), abundancebased indices(Hill numbers of order 1 and 2), and functional indices(mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could be the cause of the quasispecies complexity.RESULTS CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/mL, IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/mL, IQR 2.3-3.5)(P < 0.01) patients. The incidence and abundance indices indicated that HBV quasispecies complexity was significantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of order 2 showed statistically significant differences(CHB2.81, IQR 1.11-4.57 vs CHD 8.87, 6.56-11.18, P = 0.038). There were no significant differences in the functional indices, but CI and CHD patients also showed a trend towards greater complexity than CHB. No differences were found for any HBV quasispecies complexity indices between CHD and CI patients. G-to-A and C-to-T nucleotide changes, characteristic of APOBEC3 G, were higher in CHD and CI than in CHB in genotype A haplotypes, but not in genotype D. The proportion of nt G-to-A vs A-to-G changes and C-to-T vs T-to-C changes in genotype A and D haplotypes in CHD patients showed no significant differences. In CHB and CI the results of these comparisons were dependent on HBV genotype.CONCLUSION The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation. | Cristina Godoy David Tabernero Sara Sopena Josep Gregori Maria Francesca Cortese Carolina González Rosario Casillas Mar?al Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2019 | World Journal of Gastroenterology2019，25，13： | 6 |
| 2 | Effect of cpTi surface roughness on human bone marrow cell attachment, proliferation, and differentiation显示文摘 | Rosa AL Beloti MM | 2003 | Braz Dent J2003，14，1： | 1 |
| 3 | Osteoblastic differentiation of cultured rat bone marrow cells on hydroxyapatite with different surface topography 显示文摘 | Rosa AL Beloti MM van Noort R | 2003 | Dent Mater2003，19，： | 1 |
| 4 | Sleep deprivation affects inflammatory marker expression in adipose tissue显示文摘 | Rosa N J Lira F S Venancio D P ct al | | 0，，： | 1 |
| 5 | Seizures in cerebral vein and dural sinus thrombosis显示文摘 | Ferro JM Correia M Rosas MJ et al | 2003 | Cerebrovasc Ular Dis2003，15，12： | 1 |
| 6 | Dentin matrix proteins and soluble factors: Intrinsic regulatory signals for healing and resorption of dental and periodontal tissues显示文摘 | Silva TA Rosa AL Lara VS | 2004 | Oral Dis2004，10，2： | 1 |
| 7 | Efficacy of abioactive glass-ceramic(Biosilicate) in the main-tenance of alveolar ridges and in osseointegration oftitanium implants显示文摘 | Roriz VM Rosa AL Peitl O | 2010 | Clin Oral Implants Res2010，21，2： | 1 |
| 8 | Passive immunization with melanin-binding monoclonal antibodies prolongs survival of mice with lethal Cryptococcus neoformans infection显示文摘 | Rosas AL Nosanchuk JD Casadevall A | 2001 | Infect Immun2001，69，5： | 1 |
| 9 | Teratogen update: vitamin A congeners 显示文摘 | Rosa FW Wilk AL Kelsey FO | 1986 | Teratology1986，33，： | 1 |
| 10 | Does exercise training interfere with the effects of L - carnitine supplementation? 显示文摘 | Bacurau RF Navarro F Bassit RA Meneguello MO Santos RV Almeida AL & Costa Rosa LF | 2003 | Nutrition2003，19，： | 1 |
| 11 | In vitro cytotoxicity ofdental alloys and cpTi obtained by casting 显示文摘 | Faria AC Rosa AL Rodrigues RC | 2008 | J Biomed MaterRes B Appl Biomater2008，85，2： | 1 |
| 12 | Collagen Hydrolysate Intake Increases Skin Collagen Expression and Suppresses Matrix Metalloproteinase 2 Activity显示文摘 | Vivian Zague Vanessa de Freitas Marina da Costa Rosa et al | 2011 | J Med Food2011，14，6： | 1 |
| 13 | Passive immunization with melanin-binding monoclonal antibodies prolongs survival of mice with lethal Cryptococcus neoformans infection显示文摘 | Rosas AL Nosanchuk JD Casadevall A | | 0，，： | 1 |
| 14 | Effects of Tai Chi Chuan on cognition of elderly women with mild cognitive impairment显示文摘 | Kasai JYT Busse AL Magaldi RM Soci MA de Moraes Rosa P Curiati JAE | | 0，，： | 1 |
| 15 | H-type (hybrid) granitoids:a proposed revision of the granite-type classification and nomenclature显示文摘 | Castro A Moreno-Ventas I de al Rosa J D | 1991 | Earth-Science Reviews1991，31，： | 1 |
| 16 | Culture of osteogenic cells from human alveolar bone:A useful source of alkaline phosphatase显示文摘 | Simao AM Beloti MM Rosa AL | 2007 | Cell Bio Int2007，31，11： | 1 |
| 17 | Effect of cpTi surface roughness on human bone marrow cell attachment,proliferation,and differentiation显示文摘 | Rosa AL Beloti MM | 2003 | Braz Dent J2003，14，1： | 1 |
| 18 | 显示文摘 | Van der Salm TPM Bouwer R van Dijk AJ et aL Stimulation of scion bud release by rol gene transformed rootstocks of Rosa hybrida L | 1998 | J Exp Bot1998，49，： | 1 |
| 19 | Primary Sjogren syn- drome in Spain : clinical and immunologic expression in 1010 pa tients显示文摘 | Ramos Casals M Solan R Rosas J el al | 2008 | Medicine ( Baltimore)2008，87，4： | 1 |
| 20 | Adverse drug reactions in patients with prevention and management显示文摘 | Chinese EISENHOFER G RIVERS G ROSAS AL | 2007 | Drug Saf2007，30，11： | 1 |
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