|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The epigallocatechin gallate derivative Y_6 reverses drug resistance mediated by the ABCB1 transporter both in vitro and in vivo显示文摘Previously, we reported that Y_6, a new epigallocatechin gallate derivative, is efficacious in reversing doxorubicin(DOX)–mediated resistance in hepatocellular carcinoma BEL-7404/DOX cells. In this study, we evaluated the efficacy of Y_6 in reversing drug resistance both in vitro and in vivo by determining its effect on the adenosine triphosphate-binding cassette protein B1 transporter(ABCB1 or P-glycoprotein, P-gp). Our results showed that Y_6 significantly sensitized cells overexpressing the ABCB1 transporter to anticancer drugs that are ABCB1 substrates. Y_6 significantly stimulated the adenosine triphosphatase activity of ABCB1. Furthermore, Y_6 exhibited a higher docking score as compared with epigallocatechin gallate inside the transmembrane domain of ABCB1. In addition, in the nude mousetumor xenograft model, Y_6(110 mg/kg, intragastric administration), in combination with doxorubicin(2 mg/kg, intraperitoneal injection), significantly inhibited the growth of BEL-7404/DOX cell xenograft tumors, compared to equivalent epigallocatechin gallate. In conclusion, Y_6 significantly reversed ABCB1-mediated multidrug resistance and its mechanisms of action may result from its competitive inhibition of the ABCB1 drug efflux function. | Yan Wen Ruiqiang Zhao Pranav Gupta Yingfang Fan Yunkai Zhang Zhenguang Huang Xiaohui Li Yuangang Su Lijuan Liao Yu-An Xie Donghua Yang Zhe-Sheng Chen Gang Liang | 2019 | Acta Pharmaceutica Sinica B2019，9，2： | 3 |
| 2 | Green Control Measures of Weeds in Wheat Fields in Hetao Irrigation Area along the Yellow River显示文摘This study was conducted to solve the problem of green weed control in wheat fields in Hetao irrigation area among the Yellow River.Based on the observation of the competition between wheat and weeds in areas where weeds occurred seriously in wheat fields in Hetao irrigation area among the Yellow River,we measured the effects of green weed control measures and wheat yield using different wheat varieties,planting densities,different organic fertilizers,different ploughing times,and different mulching methods.The results showed that the emergence of weeds in wheat fields dominated by Chenopodiaceae weeds,grain amaranth and barnyard grass was more than 10 d later than wheat.Weeds were mainly distributed between rows(holes),and the number of plants accounted for 66.6%(drill seeding)and 97.6%(hole seeding),respectively.And the growth of weeds in rows(holes)was weaker,and the fresh weight of individual plants was 39.3%-41.9%lower than that between rows(holes).The ecological weed inhibitory effect was significant in the early stage of wheat growth;and among the green weed control measures,except that different varieties and planting densities caused no significant difference in weed control effect,other measures had obvious weed control effects.Comprehensive comparison showed that the control effects of plant number in black film full-covered hole seeding,conventional film-covered hole seeding,increasing ploughing times,and applying organic fertilizer free of weed seed pollution were 82.3%,71.7%,22.0%,and 8.6%,respectively;the fresh weight control effects of black film full-covered hole seeding,conventional film-covered hole seeding,increasing ploughing times,and applying organic fertilizer free of weed seed pollution were 98.0%,97.1%,23.9%,and 9.6%,respectively;and the fresh weight control effects of black film full-covered hole seeding,conventional film-covered hole seeding and increasing ploughing times increased wheat yield by 69.4%,56.4%and 21.1%,respectively.The technologies in this study can realize the purposes of mechanized green weed control in organic wheat production and low-cost,high-yield,large-scale production. | Lei YANG Jing LIU Ruiqiang WEN Peizhi ZHANG Chang LIU Aiqing WEN Jiancheng ZHANG Chunzhi ZHAO Huijuan ZHANG Hongxu ZHANG Guoqiang LI | 2021 | Agricultural Biotechnology2021，10，6： | 0 |
| 3 | A robust method for identification of plant SUMOylation substrates in a library-based reconstitution system显示文摘Dear Editor,SUMOylation,which transfers a small ubiquitin-like protein to the lysine residues of target proteins,is an important type of posttranslational modification in eukaryotic cells.This modification plays a critical role in plant development and stress responses(Miura and Hasegawa,2010);thus,the efficient identification of SUMOylation substrates in plant cells is a fundamental issue in the field.In Arabidopsis,hundreds of SUMOylated proteins have been identified by a mass spectrometry(MS)approach in which modified targets in transgenic plants harboring an H89R variant of SUMO1 are enriched via affinity chromatography and a four-residue footprint is left on the substrates after trypsin digestion for MS analysis(Miller et al.,2010).Given that antibodies generated against SUMO molecules are not very specific,it is impractical to catch endogenous SUMO moieties via antibody-based one-step purification.Thus,it is difficult to obtain SUMOylation substrates via existing approaches in plant species that cannot be efficiently transformed.Given that SUMOylation is highly reversible(Yates et al.,2016),another problem is that the modification may be removed quickly during sample preparation.In addition,based on the efficiency and sensitivity of affinity enrichment and MS,substrates with low protein levels may be missed in the analyses. | Ruiqiang Lai Wenliang Li Zewei Xu Wen Liu Qianrui Zeng Wenxiong Lin Jieming Jiang Jianbin Lai Chengwei Yang | 2023 | Plant Communications2023，4，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员