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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Carbon Coated Li4 TisOf2 Nanorods as Superior Anode Material for High Rate Lithium Ion Batteries 显示文摘 | LUO H J SHEN L F RUI K | 2013 | Journal of Alloys and Compounds2013，572，： | 1 |
| 3 | Persistence and Global Stability of a Ratio-Dependent Predator-Prey Model with Stage Structure显示文摘 | Chaplain M A J Davidson F A | 2004 | Appl Math Comput2004，158，： | 1 |
| 4 | Permanence and periodicity of a delayed ratio-dependent predator-prey model withstage structure显示文摘 | Xu Rui Chaplain M A J Davidson F A | 2005 | Math Anal Appl2005，303，： | 1 |
| 5 | Ef-fect of methyl jasmonate on quality and antioxidant activity of post-harvest loquat fruit显示文摘 | CAO S F ZHENG Y H YANG Z F WANG K T RUI H J | 2009 | Journal of the Science of Food and Agricul-ture2009，89，12： | 1 |
| 6 | Exploiting the Circulant Structure of Tracking-by-Detection with Kernels 显示文摘 | Henriques J F Rui C Martins P | 2012 | Lecture Notesin Computer Science(S0302-9743)2012，7575，1： | 1 |
| 7 | Mercury detection in air using a coated piezoelectric sensor显示文摘 | RUYS D P ANDRADE J F GUIMARAES O M | 2000 | Anal Chim Acta2000，404，1： | 1 |
| 8 | Localized application of phosphorus and ammonium improves growth of maize seedlings by stimulat- ing root proliferation and rhizosphere acidification显示文摘 | Jing J Rui Y Zhang F | 2010 | Field Crops Research2010，119，23： | 1 |
| 9 | A novel semisynthetic molecule icaritin stimulates osteogenic differentiation and inhibits adipogenesis of mesenchymal stem cells 显示文摘 | Sheng H Rui X F Sheng C J | 2013 | Int J Med Sci2013，10，6： | 1 |
| 10 | Genome structure; and expression of the ev/J family of avian endogenous viruses显示文摘 | Ruis B L Benson S J Conklin K F | 1999 | J Virol1999，73，7： | 1 |
| 11 | Global stability of a stage-structured predator-prey model with prey dispersal显示文摘 | Xu Rui CHAPLAIN M A J DAVIDSON F A | 2005 | Comput Math Appl2005，171，： | 1 |
| 12 | Commutators for the maximal and sharp functions显示文摘 | Bastero J Milman M Ruis F J | 2000 | Proc Amer Math Soc2000，11，： | 1 |
| 13 | CERPHASE:computer-generated phase diagrams显示文摘 | Ruys A J Sorrell C C Scott F H | 1990 | Journal of the Australian Ceramic Society1990，26，1： | 1 |
| 14 | Characterization of an anticorrosive phosphate suffactant and its use in waterborne coatings显示文摘 | Reyes Y Pod Riguez F J Del Rui J M | 2005 | Progress in Organic Coatings2005，，1： | 1 |
| 15 | Temperature and relative humidity effects on quality, total ascorbic acid, phenolics and flavonoid concentrations, and antioxidant activity of strawberry 显示文摘 | SHIN Y RUI H L NOCK J F | 2007 | Postharvest Biology and Technology2007，45，3： | 1 |
| 16 | Paleozoic-early Mesozoic gold deposits of the Xinjiang Autonomous Region,Nort hwestern China显示文摘 | Rui Z Goldfarb R J Qiu Y Zhou T Pirajno F Yun G | 2002 | Mineralium Deposita2002，37，： | 1 |
| 17 | Size-dependent electroluminescence from Si quantum dots embedded in amorphous SiC matrix显示文摘 | Rui Y J Li S X Xu J Song C Jiang X F Li W Chen K J Wang Q M Zuo Y H | | 0，，06： | 1 |
| 18 | Constraint based region match- ing for image retrieval显示文摘 | Wang 'F Rui Y Sun J G | 2004 | International Journal of Com- puter Vision2004，56，1： | 1 |
| 19 | Global stability of a stage -structured predator -prey mod- el with prey dispersal显示文摘 | Xu Rui Chaplain M A J Davidson F A | 2005 | Applied Mathematics and Computation2005，171，1： | 1 |
| 20 | EVIV: An end-to-end verifiable inter- net voting system显示文摘 | Rui J Paulo F Carlos R | 2013 | Computers Security2013，32，2： | 1 |
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