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| 1 | Annual cost of illness of stomach and esophageal cancer patientsin urban and rural areas in China: A multi-center study显示文摘Objective: Stomach and esophageal cancer are imposing huge threats to the health of Chinese people whereas there were few studies on the financial burden of the two cancers.Methods: Costs per hospitalization of all patients with stomach or esophageal cancer discharged between September 2015 and August 2016 in seven cities/counties in China were collected, together with their demographic information and clinical details. Former patients in the same hospitals were sampled to collect information on annual direct non-medical cost, indirect costs and annual number of hospitalization. Annual direct medical cost was obtained by multiplying cost per hospitalization by annual number of hospitalization. Annual cost of illness(ACI)was obtained by adding the average value of annual direct medical cost, direct non-medical cost and indirect cost,stratified by sex, age, clinical stage, therapy and pathologic type in urban and rural areas. Costs per hospitalization were itemized into eight parts to calculate the proportion of each part. All costs were converted to 2016 US dollars(1 USD=6.6423 RMB).Results: Totally 19,986 cases were included, predominately male. Mean ages of stomach cancer and urban patients were lower than that of esophageal cancer and rural patients. ACI of stomach and esophageal cancer patients were $10,449 and $13,029 in urban areas, and $2,927 and $3,504 in rural areas, respectively. Greater ACI was associated with male, non-elderly patients as well as those who were in stage I and underwent surgeries.Western medicine fee took the largest proportion of cost per hospitalization.Conclusions: The ACI of stomach and esophageal cancer was tremendous and varied substantially among the population in China. Preferential policies of medical insurance should be designed to tackle with this burden and further reduce the health care inequalities. | Zhixun Yang Hongmei Zeng Ruyi Xia Qian Liu Kexin Sun Rongshou Zheng Siwei Zhang Changfa Xia He Li Shuzheng Liu Zhiyi Zhang Yuqin Liu Guizhou Guo Guohui Song Yigong Zhu Xianghong Wu Bingbing Song Xianzhen Liao Yanfang Chen Wenqiang Wei Guihua Zhuang Wanqing Chen | 2018 | Chinese Journal of Cancer Research2018，30，4： | 22 |
| 2 | Adenovirus-mediated gene delivery:Potential applications for gene and cell-based therapies in the new era of personalized medicine显示文摘With rapid advances in understanding molecular pathogenesis of human diseases in the era of genome sciences and systems biology,it is anticipated that increasing numbers of therapeutic genes or targets will become available for targeted therapies.Despite numerous setbacks,efficacious gene and/or cell-based therapies still hold the great promise to revolutionize the clinical management of human diseases.It is wildly recognized that poor gene delivery is the limiting factor for most in vivo gene therapies.There has been a long-lasting interest in using viral vectors,especially adenoviral vectors,to deliver therapeutic genes for the past two decades.Among all currently available viral vectors,adenovirus is the most efficient gene delivery system in a broad range of cell and tissue types.The applications of adenoviral vectors in gene delivery have greatly increased in number and efficiency since their initial development.In fact,among over 2000 gene therapy clinical trials approved worldwide since 1989,a significant portion of the trials have utilized adenoviral vectors.This review aims to provide a comprehensive overview on the characteristics of adenoviral vectors,including adenoviral biology,approaches to engineering adenoviral vectors,and their applications in clinical and preclinical studies with an emphasis in the areas of cancer treatment,vaccination and regenerative medicine.Current challenges and future directions regarding the use of adenoviral vectors are also discussed.It is expected that the continued improvements in adenoviral vectors should provide great opportunities for cell and gene therapies to live up to its enormous potential in personalized medicine. | Cody S.Lee Elliot S.Bishop Ruyi Zhang Xinyi Yu Evan M.Farina Shujuan Yan Chen Zhao Zongyue Zeng Yi Shu Xingye Wu Jiayan Lei Yasha Li Wenwen Zhang Chao Yang Ke Wu Ying Wu Sherwin Ho Aravind Athiviraham Michael J.Lee Jennifer Moriatis Wolf Russell R.Reid Tong-Chuan He | 2017 | Genes & Diseases2017，4，2： | 19 |
| 3 | Effects of metal lead on growth and mycorrhizae of an invasive plant species (Solidago canadensis L.)显示文摘It is less known whether and how soil metal lead (Pb) impacts the invasion of exotic plants. A greenhouse experiment was conducted to estimate the effects of lead on the growth and mycorrhizae of an invasive species (Solidago canadensis L.) in a microcosm system. Each microcosm unit was separated into HOST and TEST compartments by a replaceable mesh screen that allowed arbuscular mycorrhizal (AM) fungal hyphae rather than plant roots to grow into the TEST compartments. Three Pb levels (control, 300, and 600 mg/kg soil) were used in this study to simulate ambient soil and two pollution sites where S. canadensis grows. Mycorrhizal inoculum comprised five indigenous arbuscular mycorrhizal fungal species (Glomus mosseae, Glomus versiform, Glomus diaphanum, Glomus geosporum, and Glomus etunicatum). The 15N isotope tracer was used to quantify the mycorrhizally mediated nitrogen acquisition of plants. The results showed that S. canadensis was highly dependent on mycorrhizae. The Pb additions significantly decreased biomass and arbuscular mycorrhizal colonization (root length colonized, RLC%) but did not affect spore numbers, N (including total N and 15N) and P uptake. The facilitating efficiency of mycorrhizae on nutrient acquisition was promoted by Pb treatments. The Pb was mostly sequestered in belowground of plant (root and rhizome). The results suggest that the high efficiency of mycorrhizae on nutrient uptake might give S. canadensis a great advantage over native species in Pb polluted soils. | YANG Ruyi YU Guodong TANG Jianjun CHEN Xin | 2008 | Journal of Environmental Sciences2008，20，6： | 12 |
| 4 | Evaluating efficacy of screening for upper gastrointestinal cancer in China:a study protocol for a randomized controlled trial显示文摘Objective: To evaluate the efficacy and feasibility of screening procedure for upper gastrointestinal cancer in both high-risk and non-high-risk areas in China.Setting: Seven cities/counties, representing three economical-geographical regions(Eastern, Central and Western) in China, were selected as screening centers: three in high-risk areas and four in non-high-risk areas.Participants: Villages/communities in these seven centers regarded as clusters were randomly assigned to either intervention group(screening by endoscopic examination) or control group(with normal community care) in a 1:1ratio stratified by each center. Eligible participants are local residents aged 40–69 years in the selected villages/communities with no history of cancer or endoscopic examination in the latest 3 years who are mentally and physically competent. Those who are not willing to take endoscopic examination or are unwilling to sign the consent form are excluded from the study. Totally 140,000 participants will be enrolled.Interventions: In high-risk areas of upper gastrointestinal cancer, all subjects in screening group will be screened by endoscopy. In non-high-risk areas, 30% of the subjects in screening group, identified through a survey,will be screened by endoscopy.Primary and secondary outcome measures: The primary outcome is the mortality caused by upper gastrointestinal cancer. The secondary outcomes include detection rate, incidence rate, survival rate, and clinical stage distribution. Additional data on quality of life and cost-effectiveness will also be collected to answer important questions regarding screening effects.Conclusions: Screening strategy evaluated in those areas with positive findings may be promoted nationally and applied to the majority of Chinese people. On the other hand, negative findings will provide scientific evidence for abandoning a test and shifting resources elsewhere.Trial registration: The study has been registered with the Protocol Registration System in Chinese Clinical Trial Registry. | wanqing chen hongmei zeng ru chen ruyi xia zhixun yang changfa xia rongshou zheng wenqiang wei guihua zhuang xueqin yu jie he | 2017 | Chinese Journal of Cancer Research2017，29，4： | 11 |
| 5 | Revisiting Scientific Issues for Industrial Applications of Lithium–Sulfur Batteries显示文摘Inspired by high theoretical energy density(-2600 W h kg^(-1))and cost-effectiveness of sulfur cathode,lithium–sulfur batteries are receiving great attention and considered as one of the most promising next-generation high-energy-density batteries.However,over the past decades,the energy density and reliable safety levels as well as the commercial progress of lithium-sulfur batteries are still far from satisfactory due to the disconnection and huge gap between fundamental research and practical application. | Bo Liu Ruyi Fang Dong Xie Wenkui Zhang Hui Huang Yang Xia Xiuli Wang Xinhui Xia Jiangping Tu | 2018 | Energy & Environmental Materials2018，1，4： | 9 |
| 6 | OsELF3-2, an Ortholog of Arabidopsis ELF3, Interacts with the E3 Ligase APIP6 and Negatively Regulates Immunity against Magnaporthe oryzae in Rice显示文摘Dear Editor, The ubiquitin proteasome system (UPS) is a highly regulated mechanism of intracellular protein degradation in eukaryotes.The UPS comprises three key enzymes:ubiquitin-activating enzyme E1,ubiquitin-conjugating enzyme E2,and ubiquitin ligase enzyme E3.There are nearly 1500 E3 ligase genes in rice and Arabidopsis genomes that target substrates for many biological processes for plant growth and tolerance to abiotic and biotic stresses. | Yuese Ning Xuetao Shi Ruyi Wang Jiangbo Fan Chan Ho Park Chongyang Zhang Ting Zhang Xinhao Ouyang Shigui Li Guo-Liang Wang | 2015 | Molecular Plant2015，8，11： | 8 |
| 7 | Two VOZ transcription factors link an E3 ligase and an NLR immune receptor to modulate immunity in rice显示文摘Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant immunity.However,how NLRs are regulated and activate defense signaling is not fully understood.The rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type E3 ligase AVRPIZ-T INTERACTING PROTEIN 10(APIP10)negatively regulates Piz-t accumulation.In this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONEZINC FINGER 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome pathway.OsVOZ1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in planta.The osvoz1 and osvoz2 single mutants display modest but opposite M.oryzae resistance in the non-Piz-t background.However,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to M.oryzae in the non-Piz-t background.Both OsVOZ1 and OsVOZ2 interact with Piz-t.Double silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to M.oryzae containing AvrPiz-t.Taken together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity. | Jiyang Wang Ruyi Wang Hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi Chan Ho Park Kangyu Hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang | 2021 | Molecular Plant2021，14，2： | 8 |
| 8 | Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference显示文摘Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic lineages.We previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully understood.Here,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of MSCs.Using two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus vectors.We demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in MSCs.Furthermore,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from MSCs.Thus,our findings demonstrate the important role of BMP9 in osteogenic differentiation of MSCs.The characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic signaling.Our results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics. | Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng | 2018 | Genes & Diseases2018，5，2： | 8 |
| 9 | Two selenium tolerant Lysinibacillus sp. strains are capable of reducing selenite to elemental Se efficiently under aerobic conditions显示文摘Microbes play important roles in the transport and transformation of selenium(Se) in the environment, thereby influencing plant resistance to Se and Se accumulation in plant. The objectives are to characterize the bacteria with high Se tolerance and reduction capacity and explore the significance of microbial origins on their Se tolerance, reduction rate and efficiency. Two bacterial strains were isolated from a naturally occurred Se-rich soil at tea orchard in southern Anhui Province, China. The reduction kinetics of selenite was investigated and the reducing product was characterized using scanning electron microscopy and transmission electron microscopy-energy dispersive spectroscopy. The bacteria were identified as Lysinibacillus xylanilyticus and Lysinibacillus macrolides,respectively, using morphological, physiological and molecular methods. The results showed that the minimal inhibitory concentrations(MICs) of selenite for L. xylanilyticus and L. macrolides were 120 and 220 mmol/L, respectively, while MICs of selenate for L.xylanilyticus and L. macrolides were 800 and 700 mmol/L, respectively. Both strains aerobically reduced selenite with an initial concentration of 1.0 mmol/L to elemental Se nanoparticles(SeNPs) completely within 36 hr. Biogenic SeNPs were observed both inside and outside the cells suggesting either an intra-or extracellular reduction process. Our study implied that the microbes from Se-rich environments were more tolerant to Se and generally quicker and more efficient than those from Se-free habitats in the reduction of Se oxyanions. The bacterial strains with high Se reduction capacity and the biological synthesized Se NPs would have potential applications in agriculture, food, environment and medicine. | Ju Zhang Yue Wang Zongyuan Shao Jing Li Shuting Zan Shoubiao Zhou Ruyi Yang | 2019 | Journal of Environmental Sciences2019，31，3： | 7 |
| 10 | Passively mode-locked Er-doped fiber laser based on SnS2 nanosheets as a saturable absorber显示文摘In this paper, tin disulfide(SnS_2), a two-dimensional(2D) n-type direct bandgap layered metal dichalcogenide with a gap value of 2.24 eV, was employed as a saturable absorber. Its appearance and nonlinear saturable absorption characteristics were also investigated experimentally. SnS_2-PVA(polyvinyl alcohol) film was successfully prepared and employed as a mode-locker for achieving a mode-locked Er-doped fiber laser with a pulse width of 623 fs at a pulse repetition rate of 29.33 MHz. The results prove that SnS_2 nanosheets will have wide potential ultrafast photonic applications due to their suitable bandgap value and excellent nonlinear saturable absorption characteristics. | KANGDI NIU RuYI SUN QINGYUN CHEN BAOYUAN MAN AND HUANIAN ZHANG | 2018 | Photonics Research2018，6，2： | 7 |
| 11 | Data Dynamics Enabled Privacy-Preserving Public Batch Auditing in Cloud Storage显示文摘Many schemes have been present to tackle data integrity and retrievability in cloud storage. Few of existing schemes support data dynamics, public verification and protect data privacy simultaneously. We propose a public auditing scheme which enables privacy-preserving,data dynamics and batch auditing. A data updating information table is designed to record the status information of the data blocks and facilitate data dynamics. Homomorphic authenticator and random masking technologies are exploited to protect data privacy for data owners. The scheme employs a Trusted third party auditor(TTPA)to verify the data integrity without learning any information about the data content during the auditing process.The scheme also allows batch auditing so that TTPA can process multiple auditing requests simultaneously which greatly accelerates the auditing process. Security and performance analysis show that our scheme is secure and feasible. | REN Zhengwei WANG Lina WU Qianhong DENG Ruyi | 2014 | Chinese Journal of Electronics2014，23，2： | 6 |
| 12 | The Research on Optimization of the Multiphase Flow Field of Biogas Plant by Using CFD Software显示文摘 | Ruyi Huang Yan Long Tao Luo Zili Mei Jun Wang Enshen Long | 2014 | Journal of Energy and Power Engineering2014，8，6： | 5 |
| 13 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 14 | Malware Detection Algorithm Based on the Attention Mechanism and ResNet显示文摘The cost of misclassifying a malware program as normal is often higher than that of misclassifying a normal program as malware.Therefore,how to improve the detection accuracy of malware programs is a very important problem.This paper proposes a deep learning malware program detection algorithm based on attention mechanism.Word2Vec model is used to map the Application programming interface(API)into word vectors,and all word vectors of each sample are arranged into a matrix with the same size.On this basis,residual network is used to extract features of samples.The features are input into the attention mechanism to learn the similarity between samples.Then,the features are weighted with the similarity to obtain the new features with better robustness.The new features and the original features are added element by element to obtain the sample features more suitable for classification.Finally,samples are classified by classifier.Experiments show that the classification effect of the proposed method is better than that of the traditional machine learning method. | WANG Lele WANG Binqiang ZHAO Peipei LIU Ruyi LIU Jiangang MIAO Qiguang | 2020 | Chinese Journal of Electronics2020，29，6： | 5 |
| 15 | Engineering broad-spectrum disease-resistant rice by editing multiple susceptibility genes显示文摘Rice blast and bacterial blight are important diseases of rice(Oryza sativa)caused by the fungus Magnaporthe oryzae and the bacterium Xanthomonas oryzae pv.oryzae(Xoo),respectively.Breeding rice varieties for broadspectrum resistance is considered the most effective and sustainable approach to controlling both diseases.Although dominant resistance genes have been extensively used in rice breeding and production,generating diseaseresistant varieties by altering susceptibility(S)genes that facilitate pathogen compatibility remains unexplored.Here,using CRISPR/Cas9 technology,we generated loss-of-function mutants of the S genes Pi21 and Bsr-d1 and showed that they had increased resistance to M.oryzae.We also generated a knockout mutant of the S gene Xa5 that showed increased resistance to Xoo.Remarkably,a triple mutant of all three S genes had significantly enhanced resistance to both M.oryzae and Xoo.Moreover,the triple mutant was comparable to the wild type in regard to key agronomic traits,including plant height,effective panicle number per plant,grain number per panicle,seed setting rate,and thousand-grain weight.These results demonstrate that the simultaneous editing of multiple S genes is a powerful strategy for generating new rice varieties with broadspectrum resistance. | Hui Tao Xuetao Shi Feng He Dan Wang Ning Xiao Hong Fang Ruyi Wang Fan Zhang Min Wang Aihong Li Xionglun Liu Guo-Liang Wang Yuese Ning | 2021 | Journal of Integrative Plant Biology2021，63，9： | 4 |
| 16 | A Multiresponsive Hydrophobic Associating Hydrogel Based on Azobenzene and Spiropyran显示文摘Summary of main observation and conclusion Stimuli-responsive hydrogels crosslinked by weak noncovalent bonds such as hydrogen bonds,ionic interactions,host-guest interactions and hydrophobic interactions have been extensively investigated.Herein,azobenzene and spiropyran acting as two hydrophobic pendant groups were introduced into a hydrophilic polyacrylamide (PAM) via free radical copolymerization to construct a hydrophobic interaction based thermo-,light-responsive polymer hydrogel.Its viscosity and color could be reversibly regulated by heating and cooling.Meanwhile,its color could be specifically switched by light without obvious viscosity change.The gel was also capable of being transited to sol by addition of β-cyclodextrin (β-CD),which encaged the hydrophobic groups and then increased the solubility of polymer in water.The hydrogel could be regained after UV irradiation at λ=365 nm,and after irradiation at λ=440 nm,the hydrogel changed into a clear sol again. | Anqi Liu Chenxiao Xiong Xiaoying Ma Wenjing Ma Ruyi Sun | 2019 | Chinese Journal of Chemistry2019，37，8： | 4 |
| 17 | Wheat breeding history reveals synergistic selection of pleiotropic genomic sites for plant architecture and grain yield显示文摘Diversity surveys of crop germplasm are important for gaining insights into the genomic basis for plant architecture and grain yield improvement,which is still poorly understood in wheat.In this study,we exome sequenced 287 wheat accessions that were collected in the past 100 years.Population genetics analysis identified that 6.7%of the wheat genome falls within the selective sweeps between landraces and cultivars,which harbors the genes known for yield improvement.These regions were asymmetrically distributed on the A and B subgenomes with regulatory genes being favorably selected.Genome-wide association study(GWAS)identified genomic loci associated with traits for yield potential,and two underlying genes,TaARF12 encoding an auxin response factor and TaDEP1 encoding the G-proteinγ-subunit,were located and characterized to pleiotropically regulate both plant height and grain weight.Elite single-nucleotide haplotypes with increased allele frequency in cultivars relative to the landraces were identified and found to have accumulated over the course of breeding.Interestingly,we found that TaARF12 and TaDEP1 function in epistasis with the classical plant height Rht-1 locus,leading to propose a“Green Revolution”-based working model for historical wheat breeding.Collectively,our study identifies selection signatures that fine-tune the gibberellin pathway during modern wheat breeding and provides a wealth of genomic diversity resources for the wheat research community. | Aili Li Chenyang Hao Zhenyu Wang Shuaifeng Geng Meiling Jia Fang Wang Xiang Han Xingchen Kong Lingjie Yin Shu Tao Zhongyin Deng Ruyi Liao Guoliang Sun Ke Wang Xingguo Ye Chengzhi Jiao Hongfeng Lu Yun Zhou Dengcai Liu Xiangdong Fu Xueyong Zhang Long Mao | 2022 | Molecular Plant2022，15，3： | 4 |
| 18 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 19 | Dynamic Distribution Adaptation Based Transfer Network for Cross Domain Bearing Fault Diagnosis显示文摘In machinery fault diagnosis,labeled data are always difficult or even impossible to obtain.Transfer learning can leverage related fault diagnosis knowledge from fully labeled source domain to enhance the fault diagnosis performance in sparsely labeled or unlabeled target domain,which has been widely used for cross domain fault diagnosis.However,existing methods focus on either marginal distribution adaptation(MDA)or conditional distribution adaptation(CDA).In practice,marginal and conditional distributions discrepancies both have significant but different influences on the domain divergence.In this paper,a dynamic distribution adaptation based transfer network(DDATN)is proposed for cross domain bearing fault diagnosis.DDATN utilizes the proposed instance-weighted dynamic maximum mean discrepancy(IDMMD)for dynamic distribution adaptation(DDA),which can dynamically estimate the influences of marginal and conditional distribution and adapt target domain with source domain.The experimental evaluation on cross domain bearing fault diagnosis demonstrates that DDATN can outperformance the state-of-the-art cross domain fault diagnosis methods. | Yixiao Liao Ruyi Huang Jipu Li Zhuyun Chen Weihua Li | 2021 | Chinese Journal of Mechanical Engineering2021，34，3： | 4 |
| 20 | Decoding lithium batteries through advanced in situ characterization techniques显示文摘Given the energy demands of the electromobility market,the energy density and safety of lithium batteries(LBs)need to be improved,whereas its cost needs to be decreased.For the enhanced performance and decreased cost,more suitable electrode and electrolyte materials should be developed based on the improved understanding of the degradation mechanisms and structure–performance correlation in the LB system.Thus,various in situ characterization technologies have been developed during the past decades,providing abundant guidelines on the design of electrode and electrolyte materials.Here we first review the progress of in situ characterization of LBs and emphasize the feature of the multi-model coupling of different characterization techniques.Then,we systematically discuss how in situ characterization technologies reveal the electrochemical processes and fundamental mechanisms of different electrode systems based on representative electrode materials and electrolyte components.Finally,we discuss the current challenges,future opportunities,and possible directions to promote in situ characterization technologies for further improvement of the battery performance. | Mei Yang Ruyi Bi Jiangyan Wang Ranbo Yu Dan Wang | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，5： | 4 |
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