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| 1 | Cultivation and Characterization of the MaMV-DC Cyanophage that Infects Bloom-forming Cyanobacterium Microcystis aeruginosa显示文摘The MaMV-DC cyanophage,which infects the bloom-forming cyanobacterium Microcystis aeruginosa,was isolated from Lake Dianchi,Kunming,China.Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC.Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages,and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis.Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length.Using one-step growth experiments,the latent period and burst size of MaMV-DC were estimated to be 24–48 hours and approximately 80infectious units per cell,respectively.Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA,and the genome size was estimated to be approximately 160 kb.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)analysis revealed four major structural proteins.These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium. | Tong Ou Sanhua Li Xiangyong Liao Qiya Zhang | 2013 | Virologica Sinica2013，28，5： | 8 |
| 2 | Two Virus-like Particles that Cause Lytic Infections in Freshwater Cyanobacteria显示文摘Dear Editor,We report the results of the preliminary isolation of two virus-like particles(VLPs)that are infectious to freshwater cyanobacteria from Lake Donghu,the largest urban lake in China,located in Wuhan City,Hubei Province.By light and transmission electron microscopy,we observed VLPs causing lytic infections in freshwater | Sanhua Li Tong Ou Qiya Zhang | 2013 | Virologica Sinica2013，28，5： | 3 |
| 3 | Hellebrigenin induces apoptosis in colorectal cancer Cells through induction of excessive reactive oxygen species显示文摘Traditional Chinese medicine(TCM)has been increasingly applied in both preventing and treating a variety of cancers in the last decades,attributing to its fewer side effects as compared with chemotherapy drugs.Hellebrigenin,a component of Chanpi from the skin of Bufo bufogargarizans Cantor or Duttaphrynus melanostictus has been reported to have an obvious anti-cancer activity on various cancers.However,the effect and mechanism of hellebrigenin on colorectal cancers were still unknown.Herein,the present study demonstrated hellebrigenin significantly reduced viability and triggered apoptosis via the intrinsic pathway in colorectal cancer cell lines HCT116 and HT29 in vitro and in vivo.Moreover,hellebrigenin led to a reduction of mitochondrial membrane potential.In addition,treatment with hellebrigenin could result in the induction of excessive reactive oxygen species,which led to cell apoptosis.These results indicated that hellebrigenin had anti-cancer potential in the treatment of colorectal cancers. | CHUNJIAO LIU QINHONG KONG FENG PAN SHAN JIANG LINGJIE MENG GAI HUANG LIDAN LU SANHUA LI YUN LIU | 2021 | BIOCELL2021，45，4： | 2 |
| 4 | 3-epi-bufotalin suppresses the proliferation in colorectal cancer cells through the inhibition of the JAK1/STAT3 signaling pathway显示文摘Traditional Chinese medicine(TCM)has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers.3-epi-bufotalin is an active ingredient of TCM“Chanpi”with anti-tumor potential.However,the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well disclosed.The present study demonstrated that 3-epi-bufotalin could reduce viability,trigger apoptosis,and block the cell cycle at the G2/M stage in colorectal cancer cell lines HT29,RKO,and COLO205 in vitro.Moreover,3-epi-bufotalin inhibited the JAK1/STAT3 signaling pathway.These results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells. | SANHUA LI QINGHONG KONG XIAOKE ZHANG XINTING ZHU CHUNBO YU CHANGYAN YU NIAN JIANG JING HUI LINGJIE MENG YUN LIU | 2022 | BIOCELL2022，46，11： | 2 |
| 5 | Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host显示文摘 | Ou Tong Gao Xiaochan Li Sanhua | 2015 | Journal of General Virology2015，，： | 1 |
| 6 | Mapping the antigenic determinants and reducing the immunogenicity of trichosanthin by site-directed mutagenesis显示文摘 | Qunxing An Sanhua Wei Shijie Mu Xianqing Zhang Yingfeng Lei Wei Zhang Ning Jia Xiaodong Cheng Ailing Fan Zhidong Li Zhikai Xu | 2006 | Journal of Biomedical Science2006，，5： | 1 |
| 7 | Chitosansupported imine palladacycle complex and its catalytic performance for heck reaction 显示文摘 | Xu Xia Liu Pu Li Sanhua | 2006 | React Kinet Catal Lett2006，88，2： | 1 |
| 8 | Effect of Different Silica Fumes on Properties of Al_(2)O_(3)-SiC-C Castables for Iron Trough显示文摘In order to improve the performance of Al_(2)O_(3)-SiC-C castables for iron trough,samples were prepared using brown corundum,dense corundum,activated alumina micropowder,ball pitch,and silicon as main raw materials,calcium aluminate cement as the binder.Several kinds of silica fumes(93SiO_(2),96SiO_(2),and 99SiO_(2))with different particle size distributions and chemical composition were added to research the effects on the properties of castables.The results show that the sample with 99SiO_(2) fume has high water requirement for molding,but the bulk density is the lowest,the apparent porosity is the highest,the oxidation resistance is the worst,and the permanent linear change on heating is low after firing at 1 450 ℃ for 3 h.The bulk density and the apparent porosity of the sample with 93SiO_(2) fume or 96SiO_(2) fume are equivalent,the hot modulus of rupture of the sample with 93SiO_(2) fume is the lowest,and the slag resistance of the sample with 96SiO_(2) fume is the best.The comprehensive performance of the sample with 96SiO_(2) fume is the best. | CAO Zhuang JIA Qingwei ZHANG Jun ZHOU Huijun LI Jinfeng HUN Xianlei YAN Leixin QIN Hongbin ZHANG Sanhua | 2023 | China's Refractories2023，32，2： | 0 |
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