|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 单肺通气在新生儿胸腔镜手术中的应用显示文摘目的探讨单肺通气在新生儿胸腔镜手术中的应用效果。方法择期行胸腔镜下食道重建术的先天性食道闭锁患儿60例,ASAⅡ或Ⅲ级,男31例,女29例,出生1-28d。随机均分为气管内插管组(A组)和支气管内插管组(B组)。麻醉诱导后,A组患儿在喉镜明视下完成气管插管,B组患儿在超纤细支气管内窥镜(Φ2.4mm)引导下完成普通气管导管的支气管内插管,其中左位主动脉弓者行左主支气管插管,反之,右位主动脉弓者行右主支气管插管,并于侧卧位后再次检查确认气管导管位置。记录两组患儿气管插管后5min(T_0)和人工气胸后5min(T_1)、10min(T_2)、30min(T_3)的HR、MAP、SpO_2、PETCO_2、RR、Pinsp、PEEP,并于上述各时点行动脉血气分析测得儿动脉血pH值、PaO_2、PaCO_2;评估两组患儿人工气胸后5min(T_1)、10min(T_2)的肺萎陷和手术野情况。结果 T_1-T_2时B组患儿SpO_2、PaO_2、肺萎陷和手术野评分均明显高于A组(P<0.05)。T_3时两组患儿SpO_2和PaO_2差异无统计学意义。T_0-T_3时两组患儿HR、MAP、PETCO_2、RR、Pinsp、PEEP、动脉血pH值、PaCO_2差异均无统计学意义。结论单肺通气用于新生儿胸腔镜手术,不仅安全可行,还可有效改善氧合,提高肺萎陷和手术野暴露程度。 | 胡华琨 李强 PENG Xihua XIAO Yu CHENG Liqun SHEN Shihui | 2017 | 江西医药2017，52，1： | 8 |
| 2 | 多孔沥青混合料粘弹塑性损伤模型显示文摘为合理描述多孔沥青混合料在中低温度外界荷载作用下的力学特性,基于增量型本构方程,采用Weibull损伤函数、广义Maxwell粘弹模型与D-P塑性模型,构建了粘弹塑性损伤模型.以此模型为分析手段,对不同温度和加载速率下的单轴压缩应力-应变曲线进行拟合,并分析温度与加载速率对模型参数的影响规律.分析结果表明:多孔沥青混合料粘弹参数随着温度的降低逐步退化成弹性参数,塑性模型中的体积模量和剪切模量也随温度呈现出明显的粘弹特性,塑性应变产生时对应的应变值与损伤应变阙值基本保持一致,温度及加载速率对于混合料的损伤扩展也有显著影响.构建的理论模型可以有效表征多孔沥青混合料在常温和低温下受荷时的力学损伤特性. | 易军艳 SHEN Shihui MUHUNTHAN Balasingam 冯德成 | 2014 | 哈尔滨工业大学学报2014，46，2： | 6 |
| 3 | Characteriza-tion of fatigue and healing in asphalt binders显示文摘 | Shen Shihui Chiu Homing Huang Hai | 2010 | J Mater Civil Eng2010，22，9： | 1 |
| 4 | Pervious concrete with titanium dioxide as a photocatalyst compound for a greener urban road environment显示文摘 | Shihui Shen Maria Burton Bertram Jobson Liv Haselbach | 2012 | Construction and Building Materials2012，，： | 1 |
| 5 | Multiple exosome RNA analysis methods for lung cancer diagnosis through integrated on-chip microfluidic system显示文摘Exosomes are now raising focus as a prospective biomarker for cancer diagnostics and prognosis owing to its unique bio-origin and composition.Exosomes take part in cellular communication and receptor mediation and transfer their cargos(e.g.,proteins,m RNA and DNA).Quantitative analysis of tumor-related nucleic acid mutations can be a potential method to cancer diagnosis and prognosis in early stages.Here we present an integrated microfluidic system for exosome on-chip isolation and lung cancer RNA analysis through droplet digital PCR(dd PCR).Gradient dilution experiments show great linearity over a large concentration range with R^(2)=0.9998.Utilizing the system,four cell lines and two mutation targets were parallelly detected for mutation analysis.The experiments demonstrated mutation heterogeneity and the results were agree with cell researches.These results proved our integrated microfluidic system as a promising means for early cancer diagnosis and prognosis in the era of liquid biopsy. | Yunxing Lu Zhaoduo Tong Zhenhua Wu Xiaoyu Jian Lin Zhou Shihui Qiu Chuanjie Shen Hao Yin Hongju Mao | 2022 | Chinese Chemical Letters2022，33，6： | 1 |
| 6 | Impact of aggregate packing on dynamic modulus of hot mix asphalt mixtures using three- dimensional discrete element method显示文摘 | Yu Huanan Shen Shihui | 2012 | Constrnetion and Building Materials2012，26，1： | 1 |
| 7 | Multifunctional dendrimer-entrapped gold nanoparticles for dual mode CT/MR imaging applications显示文摘 | Shihui Wen Kangan Li Hongdong Cai Qian Chen Mingwu Shen Yunpeng Huang Chen Peng Wenxiu Hou Meifang Zhu Guixiang Zhang Xiangyang Shi | 2013 | Biomaterials2013，，5： | 1 |
| 8 | Viscoelastic-plastic damage modelfor porous asphalt mixtures:Application to uniaxial compres- sion and freeze-thaw damage显示文摘 | YI Junyan SHEN Shihui | 2014 | Mechanics of Materials2014，70，： | 1 |
| 9 | Character- ization of Fatigue and Healing in Asphalt Binders显示文摘 | Shihui Shen Ho- Ming Chiu Hal Huang | 2010 | JMaterCivilEng2010，22，9： | 1 |
| 10 | Fracture Healing Properties of Asphaltic Materialunder Controlled Damage 显示文摘 | Shihui Shen Xin Lu | 2014 | Journal of Materials In Civil Engineering2014，26，： | 1 |
| 11 | Characterize Packing of Aggregate Particles for Paving Materials: Particle Size Impact显示文摘 | Shen Shihui Yu Huanan | 2011 | Con- struction and Building Materials2011，25，3： | 1 |
| 12 | Application of the Dissipated Energy Concept in Fatigue Endurance Limit Testing显示文摘 | Shen Shihui Samuel H Carpenter | 2005 | Transportation Research Record2005，1929，： | 1 |
| 13 | Fatigue Endurance Limit for Highway and Airport Pavements显示文摘 | Samuel H Carpenter Khalid A Ghuzlan Shihui Shen Pellinen | 2007 | Journal of the Transportation Research Board2007，，1： | 1 |
| 14 | Characterize packing of aggregate particles for paving materials: Particle size impact 显示文摘 | SHEN Shihui YU Huanan | 2011 | Con- struction and Building Materials2011，25，3： | 1 |
| 15 | 控制损伤条件下沥青材料的断裂愈合性能显示文摘沥青材料具有自愈性能,并通过对微损伤的修复,使沥青路面可持续使用。然而,沥青材料的自愈通常和粘弹性变形的恢复同时发生,使得沥青材料的愈合性能分析变得复杂。该文在控制材料损伤条件下,并排除粘弹性模量恢复的影响,对沥青结合料的自愈性能进行评价。首先,该文制定了一个愈合试验方案和数据分析方法,通过试验可以对沥青造成损伤并在间歇期前评定损伤等级,然后在愈合过程中监控沥青材料性能的变化和评价损伤愈合的程度;其次,建立愈合方程,在不同损伤等级及温度下模拟沥青材料的愈合性能。基于两种材料(PG 64-28、PG 70-28)的试验结果,发现在沥青材料模量长期的恢复过程中,主要受到自愈性能的影响,而粘弹性模量恢复在荷载移除后的早期就会发生,而且,沥青材料的愈合性能和疲劳性能的恢复受温度和损伤等级的影响很大。 | 朱月风(译) 张洪亮(译) Shihui Shen Xin Lu | 2016 | 中外公路2016，36，3： | 1 |
| 16 | Screening HLA-A-restricted T cell epitopes of SARS-CoV-2 and the induction of CD8^(+)T cell responses in HLA-A transgenic mice显示文摘Since severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)-specific T cells have been found to play essential roles in host immune protection and pathology in patients with coronavirus disease 2019(COVID-19),this study focused on the functional validation of T cell epitopes and the development of vaccines that induce specific T cell responses.A total of 120 CD8^(+)T cell epitopes from the E,M,N,S,and RdRp proteins were functionally validated.Among these,110,15,6,14,and 12 epitopes were highly homologous with SARS-CoV,OC43,NL63,HKU1,and 229E,respectively;in addition,four epitopes from the S protein displayed one amino acid that was distinct from the current SARS-CoV-2 variants.Then,31 epitopes restricted by the HLA-A2 molecule were used to generate peptide cocktail vaccines in combination with Poly(I:C),R848 or poly(lactic-co-glycolic acid)nanoparticles,and these vaccines elicited robust and specific CD8^(+)T cell responses in HLA-A2/DR1 transgenic mice as well as wild-type mice.In contrast to previous research,this study established a modified DC-peptide-PBL cell coculture system using healthy donor PBMCs to validate the in silico predicted epitopes,provided an epitope library restricted by nine of the most prevalent HLA-A allotypes covering broad Asian populations,and identified the HLA-A restrictions of these validated epitopes using competitive peptide binding experiments with HMy2.CIR cell lines expressing the indicated HLA-A allotype,which initially confirmed the in vivo feasibility of 9-or 10-mer peptide cocktail vaccines against SARS-CoV-2.These data will facilitate the design and development of vaccines that induce antiviral CD8^(+)T cell responses in COVID-19 patients. | Xiaoxiao Jin Yan Ding Shihui Sun Xinyi Wang Zining Zhou Xiaotao Liu Miaomiao Li Xian Chen Anran Shen Yandan Wu Bicheng Liu Jianqiong Zhang Jian Li Yi Yang Haibo Qiu Chuanlai Shen Yuxian He Guangyu Zhao | 2021 | Cellular & Molecular Immunology2021，18，12： | 0 |
| 17 | A polygalacturonase gene OsPG1 modulates water homeostasis in rice显示文摘A dynamic plant architecture is the basis of plant adaptation to changing environments.Although many genes regulating leaf rolling have been identified,genes directly associated with water homeostasis are largely unknown.Here,we isolated a rice mutant,dynamic leaf rolling 1(dlr1),characterized by‘leaf unfolding in the morning-leaf rolling at noon-leaf unfolding in the evening’during a sunny day.Water content was decreased in rolled leaves and water sprayed on leaves caused reopening,indicating that in vivo water deficiency induced the leaf rolling.Map-based cloning and expression tests demonstrated that an A1400G single base mutation in Oryza sativa Polygalacturonase 1(OsPG1)/PHOTO-SENSITIVE LEAF ROLLING 1(PSL1)was responsible for the dynamic leaf rolling phenotype in the dlr1 mutant.OsPG1 encodes a polygalacturonase,one of the main enzymes that degrade demethylesterified homogalacturonans in plant cell walls.OsPG1 was constitutively expressed in various tissues and was enriched in stomata.Mutants of the OsPG1 gene exhibited defects in stomatal closure and decreased stomatal density,leading to reduced transpiration and excessive water loss under specific conditions,but had normal root development.Further analysis revealed that mutation of OsPG1 led to reduced pectinase activity in the leaves and increased demethylesterified homogalacturonans in guard cells.Our findings reveal a mechanism by which OsPG1 modulates water homeostasis to control dynamic leaf rolling,providing insights for plants to adapt to environmental variation. | Qinwen Zou Ranran Tu Jiajun Wu Tingting Huang Zhihao Sun Zheyan Ruan Hongyu Cao Shihui Yang Xihong Shen Guanghua He Hong Wang | 2024 | The Crop Journal2024，12，1： | 0 |
| 18 | Single-cell level point mutation analysis of circulating tumor cells through droplet microfluidics显示文摘Tumor heterogeneity plays a critical role in the determination of appropriate anticancer therapy.As cir-culating tumor cells(CTCs)contain all tumor-related information,the genetic changes on CTCs could help us choose the appropriate treatments for different patients.Single-base mutations are very common in tumor genetic changes which may result in drug resistance.Here,we introduce a single-cell mutation de-tection platform based on droplet microfluidics.This platform integrates cell capsulation,cell lysis,poly-merase chain reaction(PCR)and the observation process.The droplets’generation speed is over 6000 per minute and more than 600 cells could be encapsulated in one second.To verify the performance of our platform in practical use,we performed the mutation analysis of 4 kinds of cells with our platform and noted that the genetic status of each single cell was clearly discriminated.Moreover,these results agreed with those from direct sequencing.Compared with other forms of single-cell mutation detection techniques,our platform has high throughput,short experimental time and less experimental operations. | Shihui Qiu Chuanjie Shen Xiaoyu Jian Yunxing Lu Zhaoduo Tong Zhenhua Wu Hongju Mao Jianlong Zhao | 2022 | Chinese Chemical Letters2022，33，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员