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| 1 | Small envelope protein E of SARS:cloning,expression, purification, CD determination, and bioinformatics analysis显示文摘AIM:To obtain the pure sample of SARS small envelope E protein (SARS E protein), study its properties and analyze its possible functions. METHODS: The plasmid of SARS E protein was constructed by the polymerase chain reaction (PCR), and the protein was expressed in the E coli strain. The secondary structure feature of the protein was determined by circular dichroism (CD) technique. The possible functions of this protein were annotated by bioinformatics methods, and its possible three-dimensional model was constructed by molecular modeling. RESULTS: The pure sample of SARS E protein was obtained. The secondary structure feature derived from CD determination is similar to that from the secondary structure prediction. Bioinformatics analysis indicated that the key residues of SARS E protein were much conserved compared to the E proteins of other coronaviruses. In particular, the primary amino acid sequence of SARS E protien is much more similar to that of murine hepatitis virus(MHV) and other mammal coronaviruses. The transmembrane (TM) segment of the SARS E protein is relatively more conserved in the whole protein than other regions. CONCLUSION: The success of expressing the SARS E protein is a good starting point for investigating the structure and functions of this protein and SARS coronavirus itself as well. The SARS E protein may fold in water solution in a similar way as it in membrane-water mixed environment. It is possible that β-sheet I of the SARS E protein interacts with the membrane surface via hydrogen bonding, this β-sheet may uncoil to a random structure in water solution. | SHENXu XUEJian-Hua YUChang-Ying LUOHai-Bin QINLei YUXiao-Jing CHENJing CHENLi-Li XIONGBin YUELi-Duo CAIJian-Hua SHENJian-Hua LUOXiao-Min CHENKai-Xian SHITie-Liu LIYi-Xue HUGeng-Xi JIANGHua-Liang | 2003 | Acta Pharmacologica Sinica2003，24，6： | 17 |
| 2 | Synergetic strengthening mechanism of ultrasound combined with calcium fluoride towards vanadium extraction from low-grade vanadium-bearing shale显示文摘The effect and mechanism of ultrasound and CaF_(2) on vanadium leaching from vanadium-bearing shale were investigated systematically.In consideration of the enhancement for vanadium recovery,the combination of ultrasound and CaF_(2)(66.28%) exerts more evident effects than ultrasound(26.97%) and CaF_(2)(60.35%) alone,demonstrating the synergetic effect of ultrasound and CaF_(2).Kinetic analysis manifests that the product layer diffusion controls vanadium leaching in ultrasound system without CaF_(2),however product layer diffusion and interfacial reaction is the rate-controlling step for vanadium leaching in other three leaching systems.The combination of ultrasound and CaF_(2) notably decreases the activation energy(E_(a)) from 62.03 to 27.61 kJ/mol,nevertheless individual CaF_(2) only reduces the E_(a) to 50.70 kj/mol.X-ray diffraction and fourier transform infrared spectrometer analyses show that the decomposition degree of the vanadium-bearing mica structure is the most significant in ultrasound and CaF_(2) system,proving the highest release degree of vanadium.Specific surface area and pore distribution combined with scanning electron microscope analyses reveal that the action of ultrasound and CaF_(2) would provide higher specific surface area,more abundant pores structure and cracks for the particles,which further prompts the rapid diffusion of H^(+),F^(-)and HF,and achieves the conspicuous improvement of vanadium leaching recovery. | Bo Chen Shenxu Bao Yimin Zhang | 2021 | International Journal of Mining Science and Technology2021，31，6： | 14 |
| 3 | Putative hAPN receptor binding sites in SARS_CoV spike protein显示文摘AIM:To obtain the information of ligand-receptor binding between thd S protein of SARS_CoV and CD13, identify the possible interacting domains or motifs related to binding sites, and provide clues for studying the functions of SARS proteins and designing anti-SARS drugs and vaccines. METHODS: On the basis of comparative genomics, the homology search, phylogenetic analyses, and multi-sequence alignment were used to predict CD13 related interacting domains and binding sites sites in the S protein of SARS_CoV. Molecular modeling and docking simulation methods were employed to address the interaction feature between CD13 and S protein of SARS_CoV in validating the bioinformatics predictions. RESULTS:Possible binding sites in the SARS_CoV S protein to CD13 have been mapped out by using bioinformatics analysis tools. The binding for one protein-protein interaction pair (D757-R761 motif of the SARS_CoV S protein to P585-A653 domain of CD13) has been simulated by molecular modeling and docking simulation methods. CONCLUSION:CD13 may be a possible receptor of the SARS_CoV S protein which may be associated with the SARS infection. This study also provides a possible strategy for mapping the possible binding receptors of the proteins in a genome. | YUXiao-Jing LUOCheng LinJian-Cheng HAOPei HEYou-Yu GUOZong-Ming QINLei SUJiong LIUBo-Shu HUANGYin NANPeng LIChuan-Song XIONGBin LUOXiao-Min ZHAOGuo-Ping PEIGang CHENKai-Xian SHENXu SHENJian-Hua ZOUJian-Ping HEWei-Zhong SHITie-Liu ZHONGYang JIANGHua-Liang LIYi-Xue | 2003 | Acta Pharmacologica Sinica2003，24，6： | 13 |
| 4 | Identification of probable genomic packaging signal sequence from SARS—CoV genome by bioinformatics analysis显示文摘AIM:To predict the probable genomic packaging signal of SARS-CoV by bioinformatics analysis. The derived packaging signal may be used to design antisense RNA and RNA interfere (RANi) drugs treating SARS. methods: Based on the studies about the genomic packaging signals of MHV and BCoV, especially the information about primary and secondary structures, the putative genomic packaging signal of SARS_CoV were analyzed by using bioinformatic tools. Multi-alignment for the genomic sequences was performed among SARS-CoV,MHV,BCoV, PEDV and HCoV 229E. Secondary structures of RNA sequences were also predicted for the identification fo the possible genomic packaging signals. Meanwhile, the N and M proteins of all five viruses were analyzed to study the evolutionary relationship with genomic packaging signals. RESULTS: The putative genomic packaging signal of SARS-CoV locates at the 3′ end of ORF1b near that of MHV and BCoV, where is the most variable region of this gene. The RNA secondary structure of SARS-CoV genomic packaging signal is very similar to that of MHV and BCoV. The same result was also obtained in studying the genomic packaging signals of PEDV and HCoV 229E. Further more, the genomic sequence multi-alignment indicated that the locations of packaging signals of SARS-CoV, PEDV, and HCoV overlaped each other. It seems that the mutation rate of packaging signal sequences is much higher than the N protein, while only subtle variations for the M protein. CONCLUSIONS: The probable genomic packaging signal of SARS-CoV is analogous to that of MHV and BCoV, with the corresponding secondary RNA structure locating at the similar region of ORF1b. The positions where genomic packaging signals exist have suffered rounds of mutations, which may influence the primary structures of the N and M proteins consequently. | QINLei XIONGBin LUOCheng GUOZong-Ming HAOPei SUJiong NANPeng FENGYing SHIYi-Xiang YUXiao-Jing LUOXiao-Min CHENKai-Xian SHENXu SHENJian-Hua ZOUJian-Ping ZHAOGuo-Ping SHITie-Liu HEWei-Zhong ZHONGYang JIANGHua-Liang LIYi-Xue | 2003 | Acta Pharmacologica Sinica2003，24，6： | 9 |
| 5 | Preparation of eco-friendly construction bricks from hematite tailings显示文摘 | Yongliang Chen Yimin Zhang Tiejun Chen Yunliang Zhao Shenxu Bao | 2010 | Construction and Building Materials2010，，4： | 2 |
| 6 | Effects of hepatocyte growth factor overexpressed bone marrow‐derived mesenchymal stem cells on prevention from left ventricular remodelling and functional improvement in infarcted rat hearts显示文摘 | Shenxu Wang Xing Qin Dongdong Sun Yunfang Wang Xiaoyan Xie Weiwei Fan Yabin Wang Dong Liang Xuetao Pei Feng Cao | 2012 | Cell Biochem Funct2012，，7： | 2 |
| 7 | Investigation of condition-induced bubble size and distribution in electroflotation using a high-speed camera显示文摘In the flotation process,bubble is a key factor in studying bubble-particle interaction and fine particle flotation.Knowledge on size distribution of bubbles in a flotation system is highly important.In this study,bubble distributions in different reagent concentrations,electrolyte concentrations,cathode apertures,and current densities in electroflotation are determined using a high-speed camera.Average bubble sizes under different conditions are calculated using Image-ProòPlus(Media Cyberneticsò,MD,USA)and SigmaScanòPro(Systat Software,CA,USA)software.Results indicate that the average sizes of bubbles,which were generated through 38,50,74,150,250,420,and 1000 lm cathode apertures,are 20.2,29.5,44.6,59.2,68.7,78.5,and 88.8 lm,respectively.The optimal current density in electroflotation is20 A/m2.Reagent and electrolyte concentrations,current density,and cathode aperture are important factors in controlling bubble size and nucleation.These factors also contribute to the control of fineparticle flotation. | Ren Liuyi Zhang Yimin Qin Wenqing Bao Shenxu Wang Peipei Yang Congren | 2014 | International Journal of Mining Science and Technology2014，24，1： | 2 |
| 8 | Bridging the distance: Manag- ing cross - border knowledge holders 显示文摘 | SHENXUE LI HUGH SCULLION | 2006 | Asia Pacific Journal of Management2006，，23： | 1 |
| 9 | Vanadium emission during roasting of vanadium-bearing stone coal in chlorine 显示文摘 | Zhang Yimin Hu Yangjia Bao Shenxu | 2012 | Minerals Engineering2012，30，： | 1 |
| 10 | The technology of extracting vanadium from stone coal in China: History, current status and future prospects 显示文摘 | ZHANG Yimin BAO Shenxu | 2011 | Hydrometallurgy2011，109，： | 1 |
| 11 | The technology of extracting vanadium from stone coal in China:history,current status and future prospects显示文摘 | Zhang Yimin Bao Shenxu Liu Tao | 2011 | Hydrometallurgy2011，109，： | 1 |
| 12 | The technology of extracting vanadium from stone coal in China: History, currem status and future prospects显示文摘 | ZHANG Yimin BAO Shenxu LILT Tao | 2011 | Hydrometallurgy2011，109，： | 1 |
| 13 | The technology of extracting vanadium from stone coal in China:history,current status and future prospects显示文摘 | Zhang Yimin Bao Shenxu Liu Tao | 2011 | Hydrometallurgy2011，，109： | 1 |
| 14 | Vanadium emission during roasting of vanadium-bearing stone coal in chlorine显示文摘 | Zhang Yimin Hu Yangjia Bao Shenxu | 2012 | Minerals Engineering2012，，30： | 1 |
| 15 | Pre-concentration of vanadium from stone coal by gravity separation显示文摘 | Yunliang Zhao Yimin Zhang Tao Liu Tiejun Chen Ying Bian Shenxu Bao | 2013 | International Journal of Mineral Processing2013，，： | 1 |
| 16 | Vanadium emission during roasting of vanadium-bearing stone coal in chlorine显示文摘 | Yimin Zhang Yangjia Hu Shenxu Bao | 2012 | Minerals Engineering2012，，： | 1 |
| 17 | The technology of extracting vanadium from stone coal in China : history, current status and future prospects 显示文摘 | ZHANG Yimin BAO Shenxu LIU Tao | 2011 | Hydrometallurgy2011，109，： | 1 |
| 18 | Separation factor of shaking table for vanadium pre- concentration from stone coal 显示文摘 | ZHAO Yunliang ZHANG Yimin BAO Shenxu | 2013 | Separation and Purification Technology2013，115，： | 1 |
| 19 | The technology of extracting vanadium from stone coal in China:History,current status and future prospects显示文摘 | Zhang Yimin Bao Shenxu Tao Liu | 2011 | Hydrometallurgy2011，109，： | 1 |
| 20 | Preparation of high strength autoclaved bricks from hematite tailings显示文摘 | Yunliang Zhao Yimin Zhang Tiejun Chen Yongliang Chen Shenxu Bao | 2011 | Construction and Building Materials2011，，1： | 1 |
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