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| 1 | Comparison of three PCR methods for detection of Helicobacter pylori DNA and detection of cagA gene in gastric biopsy specimens显示文摘AIM: To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and / or culture) in order to determine which of the three PCR methods (ureA, glmM and 26-kDa, SSA gene) was most appropriate in the diagnosis of Helicobacterpylon (Hpylo#) infection and also to evaluate the detection of a putative virulence marker of Hpylori,the cagA. gene, by PCR in biopsy specimens.METHODS: One hundred and eighty-nine biopsy specimens were collected from 63 patients (three biopsies each) undergoing upper gastroduodenal endoscopy for various dyspeptic symptoms. The PCR methods used to detect HpylonDNA directly from biopsies were the glmM, 26-kDa,ureA and then cagA was used to compare the culturet echnique and CLO for urease with the culture technique being used as the gold standard.RESULTS: Thirty-five percent of the biopsies were positive for Hpy/or/DNA using the 3 PCR methods, while 68% of these were positive for the cagA gene. Twenty-four percent of the biopsies were negative for Hpy/or/DNA in all PCR methods screened. The remaining 41% were either positive for urea gene only, g/mMonly, 26-kDa only, or urea + g/mM,urea + 26-kDa, glmM + 26-kDa. Out of the 35% positive biopsies, 41% and 82% were positive by culture and CLO respectively, while all negative biopsies were also negative by culture and cagA. Cag A+ infection was also predominantly found in H pylori DNA of the biopsies irrespective of the clinical diagnosis.CONCLUSION: This method is useful for correctly identifying infections caused by Hpy/or/and can be easily applied in our laboratory for diagnostic purposes. | SI Smith KS Oyedeji AO Arigbabu FCantet FMegraud OOOjo AOUwaifo JAOtegbayo SOOla AO Coker | 2004 | World Journal of Gastroenterology2004，10，13： | 5 |
| 2 | Multiplexed detection of pro- tein cancer markers with biobarceded nanoparticle probes 显示文摘 | Stoeva SI Lee JS Smith YE | 2006 | J Am Chem Soc2006，128，26： | 1 |
| 3 | High amoxycillin resistance in Helicobacter pylori isolated from gastritis and peptic ulcer patients in WesternNigeria显示文摘 | Smith SI Oyedeji KS Arigbabu AO | 2001 | J Gastroenterol2001，36，1： | 1 |
| 4 | An in situ brain perfusion technique to study cerebrovascular transport in the rat显示文摘 | Takasato Y Rapoport SI Smith QR | 1984 | Am J Physiol1984，247，32： | 1 |
| 5 | Lactobacilli in human dental caries and saliva显示文摘 | Smith SI Aweh AJ Coker AO | 2001 | Microbios2001，105，411： | 1 |
| 6 | Embryotoxicity of artesunate in animal species related to drug tissue distribution and toxicokinetic profiles显示文摘 | Li Q Si Y Smith KS | 2008 | Birth Defects Research Part B:Developmental and ReproductiveToxicology2008，83，4： | 1 |
| 7 | HLA and Mooren's ulceration显示文摘 | Taylor CJ Smith SI Morgan CH | 2000 | Br J Ophthalmol2000，84，： | 1 |
| 8 | Infectious and inflammatory processes of the spine显示文摘 | Smith AS Blaser SI | 1991 | Radiol Clin North Am1991，29，4： | 1 |
| 9 | Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes显示文摘 | Stoeva SI Lee JS Smith JE | 2006 | J Am Chen Soc2006，26，： | 1 |
| 10 | HLA and Mooren' s ulceration 显示文摘 | Taylor CJ Smith SI Morgan CH | 2000 | Br J Ophthalmol2000，84，： | 1 |
| 11 | Neuronal growth cone collapse and inhibition of protein fatty acylation by nitric oxide 显示文摘 | Hess DT Patterson SI Smith DS | 1993 | Nature1993，366，6455： | 1 |
| 12 | LIPI, a cytoplasmie Protein functionally linked to the PeutZ - Jeghers syndrome kmase LKBI显示文摘 | Smith DP Rayter SI Niederlander C | 2001 | Hum Mol Genet2001，10，： | 1 |
| 13 | Neuronal growth cone collapse and inhibition of protein fatty acylation by nitric oxide显示文摘 | Hess DT Patterson SI Smith DS | 1999 | Nature1999，336，2： | 1 |
| 14 | Sat- urable transport of manganese (1I) across the rat blood- brain barrier显示文摘 | Murphy VA Wadhwani KC Smith QR Rapoport SI | 1991 | J Neurochem1991，57，3： | 1 |
| 15 | An in situ brain perfusion technique to study cerebrovascular transport in the rat显示文摘 | Takasato Y Rapoport SI Smith QR | 1984 | Am J Physiol1984，247，32： | 1 |
| 16 | Multiplexed detection ofprotein cancer markers with biobarcoded nanoparticleprobes显示文摘 | Stoeva SI Lee JS Smith JE | 2006 | Journal of the American Chemical Society2006，128，26： | 1 |
| 17 | Sodium phenylbutyrate prolongs survival and regulates expression of anti-apoptotic genes in transgenic amyotrophic lateral sclerosis mice 显示文摘 | Ryu H Smith K Camelo SI | 2005 | J Neurochem2005，93，5： | 1 |
| 18 | Expression of the costimulatory molecule B7 on melanoma cells显示文摘 | Hersey P Si Z Smith M J | 2004 | Int J Cancer2004，58，： | 1 |
| 19 | Expression of the costimulatory molecule CD40 on melanoma cells显示文摘 | Thomas WD Smith M J Si Z | 1996 | Int J Cancer1996，68，6： | 1 |
| 20 | Cerebrovascular permeability coefficients to sodium,potassium,and chloride显示文摘 | Smith QR Rapoport SI | 1986 | J Neurochem1986，46，6： | 1 |
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