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| 1 | Comparison of three PCR methods for detection of Helicobacter pylori DNA and detection of cagA gene in gastric biopsy specimens显示文摘AIM: To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and / or culture) in order to determine which of the three PCR methods (ureA, glmM and 26-kDa, SSA gene) was most appropriate in the diagnosis of Helicobacterpylon (Hpylo#) infection and also to evaluate the detection of a putative virulence marker of Hpylori,the cagA. gene, by PCR in biopsy specimens.METHODS: One hundred and eighty-nine biopsy specimens were collected from 63 patients (three biopsies each) undergoing upper gastroduodenal endoscopy for various dyspeptic symptoms. The PCR methods used to detect HpylonDNA directly from biopsies were the glmM, 26-kDa,ureA and then cagA was used to compare the culturet echnique and CLO for urease with the culture technique being used as the gold standard.RESULTS: Thirty-five percent of the biopsies were positive for Hpy/or/DNA using the 3 PCR methods, while 68% of these were positive for the cagA gene. Twenty-four percent of the biopsies were negative for Hpy/or/DNA in all PCR methods screened. The remaining 41% were either positive for urea gene only, g/mMonly, 26-kDa only, or urea + g/mM,urea + 26-kDa, glmM + 26-kDa. Out of the 35% positive biopsies, 41% and 82% were positive by culture and CLO respectively, while all negative biopsies were also negative by culture and cagA. Cag A+ infection was also predominantly found in H pylori DNA of the biopsies irrespective of the clinical diagnosis.CONCLUSION: This method is useful for correctly identifying infections caused by Hpy/or/and can be easily applied in our laboratory for diagnostic purposes. | SI Smith KS Oyedeji AO Arigbabu FCantet FMegraud OOOjo AOUwaifo JAOtegbayo SOOla AO Coker | 2004 | World Journal of Gastroenterology2004，10，13： | 5 |
| 2 | The menin tumor suppressor protein is an essential oneogenic cofactor for MLL-associated leukemogenesis 显示文摘 | Yokoyama A Somervaille TC Smith KS | 2005 | Cell2005，123，2： | 1 |
| 3 | The menin tumor suppressor protein is an essential oncogenic cofaetor tbr MLL-associated leukemogenesis 显示文摘 | Yokoyama A Somervaille TC Smith KS | 2005 | Cell2005，123，2： | 1 |
| 4 | High amoxycillin resistance in Helicobacter pylori isolated from gastritis and peptic ulcer patients in WesternNigeria显示文摘 | Smith SI Oyedeji KS Arigbabu AO | 2001 | J Gastroenterol2001，36，1： | 1 |
| 5 | Mechanisms of action of antiepileptic drugs显示文摘 | White HS Smith MD Wilcox KS | 2007 | Int Rev Neurobiol2007，81，： | 1 |
| 6 | Separation and detection of enantiomers of stilbestrol analogues by combined high-performance liquid chromatography-thermospray mass spectrometry显示文摘 | Parker CE Levy LA Smith RW Yamaguchi K Gaskell SJ Korach KS | 1985 | J Chromatogr1985，344，： | 1 |
| 7 | Anatomy and ultrastructure of the sporophyte of Takakia ceratophylla (Bryophyta)显示文摘 | Renzaglia KS McFarland KD Smith DK | | 0，，10： | 1 |
| 8 | Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase显示文摘 | Smith DB Johnson KS | | 0，，01： | 1 |
| 9 | Expression of estrogen receptorsalpha and -beta in bladder cancer cell lines and human bladder tumor tissue显示文摘 | Shen KS Smith CL Hsieh JT | 2006 | Cancer2006，106，12： | 1 |
| 10 | Local administration of an adeno-associated viral vector expressing IL-10 reduces monocyte infiltration and subsequent photoreceptor damage during experimental autoimmune uveitis显示文摘 | Broderick CA Smith AJ Balaggan KS | 2005 | Mol Ther2005，12，： | 1 |
| 11 | lmmunotherapy of cancer 显示文摘 | Borghaei H Smith MR Campbcll KS | 2009 | Eur J Pharmacol2009，625，13： | 1 |
| 12 | Degeneration and regener-ation of the intervertebral disc:lessons from development显示文摘 | Smith LJ Nerurkar NL Choi KS | 2011 | DisModel Mech2011，1，： | 1 |
| 13 | Embryotoxicity of artesunate in animal species related to drug tissue distribution and toxicokinetic profiles显示文摘 | Li Q Si Y Smith KS | 2008 | Birth Defects Research Part B:Developmental and ReproductiveToxicology2008，83，4： | 1 |
| 14 | Environmental lead and children's intelligence: a systematic review of the epidemiological evidence显示文摘 | Pocock KS Smith M Baghurst P | 1994 | Br Med J1994，309，： | 1 |
| 15 | Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase显示文摘 | Smith DB Johnson KS | | 0，，01： | 1 |
| 16 | A phase I study of indole-3-carbinol in women:tolerability and effects显示文摘 | Reed GA Peterson KS Smith HJ | 2005 | Cancer Epidemiol Biomarkers Prev2005，14，： | 1 |
| 17 | Periodontal status and detection frequency of bacteria at sites of periodontal health and gingivitis显示文摘 | Riviere GR Smith KS Tzagaroulaki E | 1996 | J Periodontol1996，67，2： | 1 |
| 18 | Complications of foreign bodies in the esophagus 显示文摘 | Loh KS Tan LK Smith JD | 2000 | Otolaryngol Head Neck Surg2000，123，5： | 1 |
| 19 | Increasing prescription of drugs for secondary prevention after myocardial infarction显示文摘 | Channer KS | 1995 | BMJ1995，311，： | 1 |
| 20 | Diverse antiapoptotic signaling pathways activated by vasoactive intestinal polypeptide,epidermal growth factor,and phosphatidylinositol 3-kinase in prostate cancer cells converge on BAD显示文摘 | Sastry KS Smith AJ Karpova Y | 2006 | J Biol Chem2006，281，20： | 1 |
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