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		题名	作者	年代	出处	被引量
	1	Enhancement of transfection efficiency for HeLa cells via incorporating arginine moiety into chitosan显示文摘Arginine-rich peptides have attracted considerable attention due to their distinct internalization mechanism. It was reported that arginine and guanidino moieties were able to translocate through cell membranes and played a critical role in the process of membrane permeation. In this work, arginine was conjugated to the backbone of chitosan to form a novel chitosan derivative, arginine modified chitosan (Arg-CS). Arg-CS/DNA complexes were prepared according to the method of coacervation process. The physicochemical properties of Arg-CS and Arg-CS/DNA complexes were characterized and the transfection activity and efficiency mediated by Arg-CS/DNA complexes were investigated taking HeLa cells as target cells. Arg-CS was characterized by FTIR and 13C NMR. Arg-CS/DNA polye- lectrolyte complexes were investigated by agarose gel retardation, dynamic light scattering (DLS) and atomic force microscopy (AFM). The results revealed that the Arg-CS/DNA complexes started to form at N/P ratio of 2:1, and the size of particles varied from 100 to 180 nm. The cytotoxicity of Arg-CS and their complexes with plasmid DNA were determined by MTT assay for HeLa cells, and the results suggested that Arg-CS/DNA complexes were slightly less toxic than Arg-CS. Moreover, the derivative alone and their complexes showed significantly lower toxicity than PEI and PEI/DNA complexes, respectively. Taking HeLa cells as target cells and using pGL3-control as reporter gene, the luciferase expression mediated by Arg-CS was greatly enhanced to about 100 folds compared with the luciferase expression mediated by chitosan at different pH media. These results suggest that Arg-CS is a promising candi- date as a safe and efficient vector for gene delivery and transfection.	ZHU DunWan ZHANG HaiLing BAI JinGen LIU WenGuang LENG XiGang SONG CunXian YANG Jian LI XiaoWei JIN Xu SONG LiPing LIU LanXia LI XiuLan ZHANG Yang YAO KangDe	2007	Chinese Science Bulletin2007，52，23：	4
	2	Arterial uptake of biodegradable nanoparticles for intravascular local drug delivery:results with an acute dog model显示文摘	Song Cunxian Vinod Labhasetwar Cui Xiumin	1998	J Controll Release1998，54，：	1
	3	A new type gene delivery system-gene nanoparticles显示文摘	Yang Jing Song Cunxian Sun Hongfan	2005	Key Eng Mater2005，，：	1
	4	A new type gene delivery system gene nanoparticles 显示文摘	Yang Jing Song Cunxian Sun Hongfan	2005	Key Eng Mater2005，288，：	1
	5	The incorporation of an ion channel gene mutation associated with the long QT syndrome(Q9E-hMiRP1)in a plasmid vector for site-specific arrhythmia gene therapy:in vitro and in vivo feasibility studies显示文摘	Burton DY Song Cunxian Fishbein I	2003	Hum Gene Ther2003，14，9：	1
	6	显示文摘	Sun Hongfan Mei Lin Song Cunxian	2006	Biomaterials2006，27，9：	1

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