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| 1 | Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine显示文摘Erysiphe necator is the causal agent of powdery mildew(PM),one of the most destructive diseases of grapevine.PM is controlled by sulfur-based and synthetic fungicides,which every year are dispersed into the environment.This is why PM-resistant varieties should become a priority for sustainable grapevine and wine production.PM resistance can be achieved in other crops by knocking out susceptibility S-genes,such as those residing at genetic loci known as MLO(Mildew Locus O).All MLO S-genes of dicots belong to the phylogenetic clade V,including grapevine genes VvMLO7,11 and 13,which are upregulated during PM infection,and VvMLO6,which is not upregulated.Before adopting a gene-editing approach to knockout candidate S-genes,the evidence that loss of function of MLO genes can reduce PM susceptibility is necessary.This paper reports the knockdown through RNA interference of VvMLO6,7,11 and 13.The knockdown of VvMLO6,11 and 13 did not decrease PM severity,whereas the knockdown of VvMLO7 in combination with VvMLO6 and VvMLO11 reduced PM severity up to 77%.The knockdown of VvMLO7 and VvMLO6 seemed to be important for PM resistance,whereas a role for VvMLO11 does not seem likely.Cell wall appositions(papillae)were present in both resistant and susceptible lines in response to PM attack.Thirteen genes involved in defense were less upregulated in infected mlo plants,highlighting the early mlo-dependent disruption of PM invasion. | Stefano Pessina Luisa Lenzi Michele Perazzolli Manuela Campa Lorenza Dalla Costa Simona Urso Giampiero Valè Francesco Salamini Riccardo Velasco Mickael Malnoy | 2016 | Horticulture Research2016，3，1： | 7 |
| 2 | Gene structure and expression analysis of the drought-and abscisic acid-responsive CDeT11-24 gene family from the resurrection plant Craterostigma plantagineum Hochst显示文摘 | VELASCO R SALAMINI F BARTELS D | 1998 | Planta1998，204，4： | 1 |
| 3 | Knock-out of the plastid ribosomal protein L11 in Arabidopsis: effects on mRNA translation and photosynthesis显示文摘 | Pesaresi P Varotto C Meurer J Jabns P Salamini F Leister D | 2001 | Plant J2001，27，3： | 1 |
| 4 | Structural analysis of the Waxy lus from Hordeum vulgare显示文摘 | Rohde W Becker D Salamini F | 1988 | Nucleic Acids Research1988，，： | 1 |
| 5 | RFLP maps of potato and their alignment with the homoeologous tomato genome显示文摘 | C. Gebhardt E. Ritter A. Barone T. Debener B. Walkemeier U. Schachtschabel H. Kaufmann R. D. Thompson M. W. Bonierbale M. W. Ganal S. D. Tanksley F. Salamini | 1991 | Theoretical and Applied Genetics1991，，1： | 1 |
| 6 | A PCR-based ap- proach for isolating pathogen resistance genes from potato with potential for wide application in plants 显示文摘 | Leister D Ballvora A Salamini F | 1996 | Nature Gen- et1996，14，： | 1 |
| 7 | The mlo resistance al- leles to powdery mildew infection in barley trigger a developmental- ly controlled defence mimic phenotype显示文摘 | Woher M Hollricher K Salamini F | 1993 | Molecular and General Genetics MGG1993，239，12： | 1 |
| 8 | Structural analysis of the Waxy locus from Hordeum vulgare显示文摘 | Rohde W Becker D Salamini F | 1988 | Nucleic Acids Research1988，，16： | 1 |
| 9 | Structure of a chalcone synthase gene from Hordeum vulgate显示文摘 | Rohde W D6rr S Salamini F | 1991 | Plant MolecularBiology1991，16，6： | 1 |
| 10 | A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants显示文摘 | Leister D Allvora A Salamini F | 1996 | Nat Genetics1996，14，： | 1 |
| 11 | Expression of desiccation related proteins from the resurrection plant Craterostigma plantagineum in transgenic tobacco 显示文摘 | Iturriaga G Schneider K Salamini F | 1992 | Plant Molecular Biology1992，20，： | 1 |
| 12 | Estimation of recombination frequencies in crosses and construction ofRFLP linkage maps in plants from crosses between heterozygous parents 显示文摘 | Ritter E Gebhardt C Salamini F | 1990 | Genetics1990，，125： | 1 |
| 13 | , Structure and regulation of an ABA- and desiccation-responsive gene from the resurrection plant Craterostigula plantagineum显示文摘 | MICHEL D FURINI A SALAMINI F | 1994 | Plant Mol Bio11994，24，： | 1 |
| 14 | A prediction of the size and evolutionary origin of the proteome of chloroplasts of Arabidopsis 显示文摘 | Abdallah F Salamini F Leister D | 2000 | Trends Plant Sci2000，5，4： | 1 |
| 15 | The R1 gene conferring reca-specific resistance to Phytophthora infestans in potato is located on chromosome V显示文摘 | Leonards-Schippers C Gieffers W Salamini F | 1992 | Mol Gen Genet1992，，233： | 1 |
| 16 | A PCRbased approach for isolating pathogen resistance genes from potato with potential for wide application in plants 显示文摘 | LEISTER D BALLVORA A SALAMINI F | 1996 | Nat Genet1996，14，4： | 1 |
| 17 | R6 and R7 allels of potato conferring race-specific resistance to Phytophthora infestans identified genetic loci clustering with the R3 locus on chromosome XI显示文摘 | E1-Knarbotly A Palomino Sanchez C Salamini F | 1996 | Theor Appl Genet1996，，92： | 1 |
| 18 | A PCR-based approach for isolating pathogen resistance gene from potato with potential for application in plants显示文摘 | Leister D Ballvora A Salamini F | 1996 | Nature Genet1996，14，： | 1 |
| 19 | Cytoplasmic N-terminal protein acetylation is required for efficient photosynthesis in Arabidopsis显示文摘 | Pesaresi P Gardner NA Masiero S Dietzmann A Eichacker L Wickner R Salamini F Leister D | | 0，，： | 1 |
| 20 | The m/o resistance alleles to powdery mildew infection in barley trigger a developmentally controlled defence mimic phenotype显示文摘 | Wolter M Hollricher K Salamini E | 1993 | Mol Gen Genet1993，239，12： | 1 |
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