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| 1 | Cloning of first abc transporter encoding gene from Trichoderma spp. and its expression during stress and mycoparasitism显示文摘Trichoderma in its natural environment competes for nutrient uptake and is required to protect itself from adverse natural toxic compounds, such as those produced by plants and other microbes in the soil community, or synthetic toxic compounds released human activity. One of the most important metabolic pathways for drug resistance and substrate uptake, both in prokaryotes and eukaryotes, is ATP dependent. The role of ABC transporter proteins in the biology of Trichoderma is still not known. We present the cloning of the first four ABC transporter genes (TABC1, TABC2, TABC3, TABC4 ) in Trichoderma, and in particular T. atroviride P1, and the characterization of TABC2 The complete sequence of this gene is 6535 bp, which includes a promoter of 1624 bp, a terminator of 642 bp and a coding region of 4264 bp. The promoter contains many of the potential transcription factor binding sites found in the 5’ upstream region of the ech42 gene of T. atroviride P1. These included: heat shock factors (HSF), a nitrogen-regulating factor (Nit-2), a stress-response element (STRE), a GCR1 elements, and a Cre BP1 motif. Northern analysis and RT-PCR demonstrated that TABC2 is highly expressed when Trichoderma is subjected to nitrogen starvation, grown in the presence of culture filtrates of Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum, or when N-acetylglucosamine is added to the substrate. TABC2 appears to be co-regulated with some CWDE-encoding genes, suggesting that this is the first ABC transporter encoding gene involved in mycoparasitic events. It’s role in the interaction of Trichoderma with fungal hosts or plants is being investigated by targeted gene disruption and overexpression. | Lanzuise S Ruocco M Scala V Catapano L Woo S Ciliento R Ferraioli S Soriente I Vinale F Scala F Del Sorbo G Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | HCV-related hepato-cellular carcinoma:from chronic inflammation to cancer显示文摘 | Castello G Scala S Palmieri G | 2010 | Clin Immunology2010，134，3： | 1 |
| 3 | Correlations in the bound future market显示文摘 | Cuniberti G Raberto M Scalas E | 1999 | Physica A1999，269，1： | 1 |
| 4 | Puttheservice-pmfitchaintovo&显示文摘 | Heskett J L Jmes T O Loveman G W Sasserr W E Jr ScaLA | 1994 | Har-vard Business Review1994，72，2： | 1 |
| 5 | Heat transfer mea-surements and simulation of KATAPAK-M (R) catalyst supports显示文摘 | Von Scala C Wehrli M Gaiser G | 1999 | Chemical Engineering Science1999，54，10： | 1 |
| 6 | Carbon dioxide emission related to chemical properties of a tropical bare soil显示文摘 | La Scala N Marques J Pereira G | 2000 | Soil Biology and Biochemistry2000，32，10： | 1 |
| 7 | Plasma PAI21 levels are increased in patients with nonalcoholic steatohepatitis 显示文摘 | WARGHER G BERTOLINI L SCALA L | 2007 | Diabetes Care2007，30，5： | 1 |
| 8 | MicroRNA profiling reveals that miR -21, miR486 and miR-214 are upregulated and involved in cell survival in Sezary syndrome 显示文摘 | Narducci M G Arcelli D Picchio M C Lazzeri C Pagani E Sampogna F Scala E Fadda P Cristofoletti C Facchiano A Frontani M Monopoli A Ferracin M Negrini M Lombardo G A Caprini E Russo G | 2011 | Cell Death Disease2011，2，4： | 1 |
| 9 | Modelling short-term temporal changes of bare soil CO2 emissions in a tropical agrosystem by using meteorological data显示文摘 | Scala N L Panosso A R Pereira G T 2003 | 2003 | Applied Soil Ecology2003，24，： | 1 |
| 10 | Cystic echinoeoccosis in Italy from the 1950s to present显示文摘 | Garippa G Varcasia A Scala A | 2004 | Parassitologia2004，46，4： | 1 |
| 11 | A survey of Taenia multiceps coenurosis in Sardinian sheep显示文摘 | SCALA A CANCEDDA G M VARCASIA A | 2007 | Vet Parasitol2007，143，34： | 1 |
| 12 | Short-texan soil CO2 emission after conventional and reduced tillage of a no-till sugar cane area in southern Brazil 显示文摘 | SCALA JR N LA BOLONGEZI D PEREIRA G T | 2006 | Soil and Tillage Research2006，91，： | 1 |
| 13 | Selection of HIV-specific immunogenic epitopes by screening random peptide libraries with HIV-1-positive sera 显示文摘 | Scala G Chen X Liu W | 1999 | J Immunol1999，162，10： | 1 |
| 14 | Noninvasive Versus conventional Ventilation to treat hypercapnic encephalopathy in chronic obstructive pulmonary disease 显示文摘 | Scala R Nava S Conti G | 2007 | Intensive Care Med2007，33，12： | 1 |
| 15 | Half dose thrombolysis to begin with ,when immediate coronary angioplasty in acute myocardial infarction is not possible显示文摘 | La Scala E Steffenino G Dellavalle A | 2004 | Ital Heart J2004，5，： | 1 |
| 16 | Noninvasive versus conventional ventilation to treat hypercapnic encephalopathy in chronic obstructive pulmonary disease显示文摘 | Scala R Nava S Conti G | 2007 | Intensive Care Med2007，33，12： | 1 |
| 17 | Higher PV module efficiency by a novel CBS bypass显示文摘 | ACCIARI G GRACI D LA SCALA A | 2011 | IEEE Transactions on Power Electronics2011，26，5： | 1 |
| 18 | Half-dose thrombolysis to begin with,when immediate coronary angioplasty in acute myocardial infarction is not possible显示文摘 | La Scala E Steffenino G Dellavalle A | 2004 | Ital Heart J2004，5，9： | 1 |
| 19 | Noninvasive versus con-ventional ventilation to treat hypercapnic encephalopathyin chronic obstructive pulmonary disease显示文摘 | Scala R Nava S Conti G | 2007 | Intensive CareMed2007，33，2： | 1 |
| 20 | Adenovirusmediated suppression of HMGI ( Y ) protein synthesis as potential therapy of human malignant neoplasias显示文摘 | Scala S Portella G Fedele M | 2000 | Proc Natl Acad Sci USA2000，97，8： | 1 |
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