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| 1 | Immune response-related genes associated to blocking midgut dengue virus infection in Aedes aegypti strains that differ in susceptibility显示文摘Aedes (Stegomyia) aegypti, the principal global vector of dengue viruses, has difTerences in its susceptibility to dengue virus infection. We compared the global expres? sion of genes in the midguts of Colombian Ae. aegypti dengue-susceptible (Cali-S) and dengue-refractory (Cali-MIB) field derived strains after ingesting either a sugarmeal, a bloodmeal, or a bloodmeal containing dengue virus serotype 2 (DENV-2). Microarraybased transcriptome analysis among treatments indicated a total of 4725 transcripts with differential expression between the two strains. Eleven genes were selected from different functional groups based on their significant up or down expression levels as well as reports in the literature suggesting they are associated with dengue virus elimination. We measured mRNA abundance of these 11 genes at 0, 8, 24, and 36 h postinfection using quantitative real time PCR (qPCR) to confirm the microarray results and assess any temporal patterns. Four genes were selected (Gram-negative binding protein—GNBP [AAEL009176], Niemann Pick Type-C2一NPC2 [AAEL015136], Keratinocyte lectin [AAEL009842], and Cathepsin-b [AAEL007585]) for knockdown experiments using RNA interference (RNAi) methodology to determine the phenotype (DENV-2 susceptible or refractory). Silencing GNBP、Cathepsin-b and Keratinocyte lectin reduced the percentage of mosquitoes with disseminated virus in the Cali-S strain to 8%, 20%, and 12% respectively compared with 96% in the controls. Sile ncing of NPC2 increased the percentage of mosquitos with disseminated virus infections in Cali-MIB to 66% compared with 35% in the controls. This study provides in sight into genes that may contribute to the Cali-S susceptible and Cali-MIB refractory phenotypes in 昇匕 aegypti. | Paola A. Caicedo Idalba Mildred Serrato Shuzhen Sim George Dimopoulos Heather Coatsworth Carl Lowenberger Clara B. Ocampo | 2019 | Insect Science2019，26，4： | 2 |
| 2 | Effect of type of dietary fat on induces of immune status of rabbit显示文摘 | Kelley D S Nelson G J Serrato C M | 1988 | Journal of Nutrition1988，18，： | 1 |
| 3 | Structuralcharacterization of an acidic exoheteropolysaccharide produced by the nitrogen-fixing bacterium Burkholderia tropica显示文摘 | SERRATO R V SASSAKI G L GORIN P A J | 2008 | Carbohydrate Polymers2008，73，4： | 1 |
| 4 | Type-h thioredoxins accumulate in the nucleus of developing wheat seed tissues suffering oxidative stress显示文摘 | SERRATO A J CEJUDO F J | 2003 | Planta2003，7，： | 1 |
| 5 | ERG findings in patients using hydroxychloroquine显示文摘 | Tzekov RT Serrato A Marmor MF | | 0，，01： | 1 |
| 6 | A variant of human paraoxonase/ arylesterase (HUMPONA) gene is a risk factor for coronary disease显示文摘 | Marian AJ | 1995 | J Clin Invest1995，96，： | 1 |
| 7 | Effect of lubricant viscosity grade on mechanical vibration of roller bearings显示文摘 | Serrato R Maru M M Padovese L R | | 0，，08： | 1 |
| 8 | Effects of type of dietary fat on indices of immune sta- tus of rabbits 显示文摘 | KELLEY D S NELSON G J SERRATO C M | 1988 | The Journal of Nutrition1988，118，11： | 1 |
| 9 | Effect of stirring on oxide skins and porosity inA356 aluminum alloy 显示文摘 | Serratos Poirier M Lyman | 2000 | AFS Trans2000，，108： | 1 |
| 10 | A simple technique for measuring rainfall interception by small shrub: 'interception flow collection box'显示文摘 | SERRATO F B DIAZ A R | 1998 | Hydrological Processes1998，12，： | 1 |
| 11 | Sanal charaacL-nizafion of an acidic exoheteropoly saccharide produced by the nitrogen-fixing bacterium Burkholderia tropica显示文摘 | SERRATO R V SASSAKI G L GOR/N P A J | 2008 | Carbohydrate Polymers2008，73，4： | 1 |
| 12 | Isolation and partial characterization of a pectic polysaccharide from the fruit pulp of Spondias cytherea and its effect on peritoneal macrophage activation显示文摘 | Iacomini M Serrato R V Lopes L | 2005 | Fitoterapia2005，76，： | 1 |
| 13 | Hetero- geneous conditions in dissolved oxygen affect N glycosylation but not productivity of a monoclonal antibody in hybridoma cultures 显示文摘 | Serrato J A Palomares L A Meneses-Acosta | 2004 | Biotechnology and Bioengineering2004，88，2： | 1 |
| 14 | A simple technique for measuring rainfall interception by small shrub 'interception flow collection box'显示文摘 | Serrato F B Diaz A R | 1998 | Hydrological Processes1998，12，： | 1 |
| 15 | Effect of lubricant viscosity grade on mechanical vibration of roller bearings 显示文摘 | Serrato R Maru M M | 2007 | Tribology International2007，40，： | 1 |
| 16 | Plufipotential differentiation capability of human adiposc-derived stem cells in a novel fibrin-agarose scaffold显示文摘 | NIETO-AGUILAR R SERRATO D GARZON I | 2010 | J Biomater Appl2010，17，： | 1 |
| 17 | The role of echography in osteolytic tubercular abscesses显示文摘 | Gandolfo N Serrato O Sandrone C | 1993 | Radiol Med (Torino)1993，85，5： | 1 |
| 18 | A variant of human paraoxonase/arylesterase (HUPONA)gene is a risk factor for coronary artery disease 显示文摘 | Serrato M Marian AJ | 1995 | J Clin Invest1995，，： | 1 |
| 19 | Type-h thioredoxins accumulate in the nucleus of developing wheat seed tissues suffering oxidative stress显示文摘 | Serrato A J Cejudo F J | 2003 | Planta2003，217，： | 1 |
| 20 | Type-h thioredoxins accumulate in the nucleus of developing wheat seed tissues suffering oxidative stress 显示文摘 | ANTONIO J SERRATO FRANCISCO J | 2003 | Planta2003，217，： | 1 |
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