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| 1 | ZnO-based micromotors fueled by CO_(2):the first example of self-reorientation-induced biomimetic chemotaxis显示文摘Synthetic chemotactic micro/nanomotors are envisioned to actively‘seek out’targets by following specific chemicals,but they are mainly powered by bioincompatible fuels and only show pseudochemotaxis(or advanced chemokinesis)due to their weak self-reorientation capabilities.Here we demonstrate that synthetic ZnO-based Janus micromotors can be powered by the alternative biocompatible fuel of C0_(2);and further provide the first example of self-reorientation-induced biomimetic chemotaxis using them.The ZnO-based micromotors are highly sensitive to dissolved C0_(2) in water,which enables the corrosion of ZnO to continuously occur by providing H+through hydration.Thus,they can autonomously move even in water exposed to air based on self-diffusiophoresis.Furthermore,they can sense the local C0_(2) gradient and perform positive chemotaxis by self-reorientations under the phoretic torque.Our discovery opens a gate to developing intelligent micro/nanomotors powered by,and sensitive to,biocompatible atmospheric or endogenous gaseous chemicals for biomedical and environmental applications. | Fangzhi Mou Qi Xie Jianfeng Liu Shengping Che Lamya Bahmane Ming You Jianguo Guan | 2021 | National Science Review2021，8，11： | 3 |
| 2 | Tandem Biocatalysis by CotA-TJ102@UIO-66-NH2 and Novozym 435 for Highly Selective Transformation of HMF into FDCA显示文摘2,5-Furandicarboxylic acid (FDCA) is a potential biorenewable chemical for applications including plastics, polyamides, drugs, etc. The selective biosynthesis of FDCA from 5-hydroxymethylfurfural (HMF) by a speci c enzyme poses a great challenge. In this study, we reported an e cient strategy to produce FDCA from HMF by the tandem biocatalysis of laccase (CotA-TJ102@UIO-66-NH 2 ) and Novozym 435. For the rst step, a nanoparticle metal organic framework was synthesized as a carrier to immobilize CotA-TJ102@UIO-66-NH 2 , which was assigned for the production of 5-formyl-2-furancarboxylic acid (FFCA) and featured an enzyme loading of 255.54 mg/g, speci c activity of 135.90 U/mg, and solid loading ratio of 99.65%. Under optimal conditions, an ideal FFCA yield of 98.5% was achieved, and the CotA-TJ102@UIO-66-NH2 pre- sented a high recycling capacity after 10 cycles. For the second step, Novozym 435 was applied for the further conversion of FFCA into FDCA, presenting a high FDCA yield of 95.5% under the optimized conditions. Novozym 435 also exhibited a high recyclability after eight cycles. As a result, the tandem biocatalysis strategy provided a 94.2% FDCA yield from HMF, indicating its excellence as a method for FDCA production. | Xin Chang Chengyu Zhang Lan Gao Xiao Liu Shengping You Wei Qi Kang Wang Xin Guo Rongxin Su Han Lu Zhimin He | 2019 | Transactions of Tianjin University2019，25，5： | 2 |
| 3 | Kinetic modeling of gamma-aminobutyric acid production by Lactobacillus brevis based on pH-dependent model and rolling correction显示文摘Gamma-aminobutyric acid(GABA)is a natural non-protein functio nal amino acid,which has potential for fermentation industrial production by Lactobacillus brevis.This work investigated the batch fermentation process and developed a kinetic model based on substrate restrictive model established by experimental data from L25(5~6)orthogonal experiments.In this study,the OD600 value of fermentation broth was fixed to constant after reaching its maximum because the microorganism death showed no effect on the enzyme activity of glutamate decarboxylase(GAD).As pH is one of the key parameters in fermentation process,a pH-dependent kinetic model based on radial basis function was developed to enhance the practicality of the model.Furthermore,as to decrease the deviations between the simulated curves and the experimental data,the rolling correction strategy with OD600 values that was measured in real-time was introduced into this work to modify the model.Finally,the accu racy of the rolling corrected and pH-dependent model was validated by good fitness between the simulated curves and data of the initial batch fermentation(pH 5.2).As a result,this pH-dependent kinetic model revealed that the optimal pH for biomass growth is 5.6-5.7 and for GABA production is about 5,respectively.Therefore,the developed model is practical and convenient for the instruction of GABA fermentation production,and it has instructive significance for the industrial scale. | Min Wu Juanjuan Ding Zhaofeng Zhang Shengping You Wei Qi Rongxin Su Zhimin He | 2022 | Chinese Journal of Chemical Engineering2022，，10： | 1 |
| 4 | Development of an integrated process for the production of high-purityγ-aminobutyric acid from fermentation broth显示文摘γ-Aminobutyric acid(GABA),a natural non-protein amino acid,plays an irreplaceable role in regulating the life activities of organisms.Nowadays,the separation and purification of food-grade GABA from fermentation broth is still a great challenge.This research utilized monosodium glutamate as a substrate for the production of high-purity GABA via an integrated process incorporating fermentation,purification,and crystallization.Firstly,147 g·L^(-1) GABA with a yield of 99.8%was achieved through fed-batch fermentation by Lactobacillus brevis CE701.Secondly,three integrated purification methods by ethanol precipitation were compared,and crude GABA with a purity of 89,85%was obtained by the optimized method.Thirdly,GABA crystals with a purity of 98.69%and a yield of 60%were further obtained through a designed crystallization process.Furthermore,the GABA industrial production process model was established by Superproper Designer V10 software,and material balance and economic analysis were carried out.Ethanol used in the process was recovered with a recovery of 98.79%through Aspen simulated extractive distillation.Then the fixed investment(equipment purchase and installation costs)for an annual production of 80 t GABA will be about 833000 USD;the total annual production cost(raw material cost and utility cost)will be about 641000 USD.The annual sale of GABA may be at the range of 2400000-4000000 USD and the payback period will be about 1-2 year.This integrated process provides a potential way for the industrial-scale production of food-grade GABA. | Zhaofeng Zhang Juanjuan Ding Min Wu Bochao Liu Huiwen Song Shengping You Wei Qi Rongxin Su Zhimin He | 2022 | Chinese Journal of Chemical Engineering2022，，10： | 0 |
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