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您的检索式：作者名="Shentong Wang"
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| 1 | A multiplex microsatellite PCR method for evaluating genetic diversity in grass carp(Ctenopharyngodon idellus)显示文摘Grass carp is one of the most important cultured fishes all over the world.The genetic diversity of grass carp plays an important role whatever in selective breeding progress or ecological conservation purposes.However,some genetic diversity researches were not accuracy and cannot be compared with each other due to different molecular markers,sample size and detection methods.In this study,we constructed five multiplex PCR assays contained 20 microsatellites with highly polymorphism and heterozygosity for evaluating genetic information of grass carp.We used nine cultured populations consisting of 507 individuals to detect stability of the five multiplex PCR assays.The results showed that the number of alleles(Na),effective number of alleles(Ne),observed heterozygosity(Ho),expected heterozygosity(He)and polymorphism information content(PIC)of the 20 microsatellite loci were relative high compared with the genetic diversity parameters of microsatellite loci developed by other researchers.Six loci were significantly deviated from Hardy-Weinberg equilibrium(P<0.01).And with exception of the Shaoguan,Indian and Nepal cultured population,all other cultured populations showed very high genetic diversity.Through the test of grass carp populations,we developed an effective and accurate multiplex SSR-PCR assays that can be as statistical powerful tool for detecting genetic information of grass carp. | Da Li Shentong Wang Yubang Shen Xinzhan Meng Xiaoyan Xu Rongquan Wang Jiale Li | 2018 | Aquaculture and Fisheries2018，3，6： | 4 |
| 2 | Characterization of two splice variants of EGFR and their effects on the growth of the razor clam显示文摘The epidermal growth factor receptor(EGFR)plays a vital role in cell growth,proliferation,and body growth.In this study,two EGFR isoforms(Sc-EGFR-1a and Sc-EGFR-1b)were obtained from Sinonovacula constricta.Sequence analysis of the coding region of Sc-EGFRs,revealed the 2 isoforms were generated from a common gene by alternative splicing and that one form possessed an extra 81 bp that corresponded to an insertion of 27 amino acids in the C-terminus.Expression of the Sc-EGFR isoforms were detectable in the early embryo stages and increased in the middle-late stages.Both isoforms were widely expressed in tissues,but the highest levels were detected in the siphon.Although both isoforms had a typical extracellular region,transmembrane region,and intracellular region,the insertion/deletion of 27 aa in the C-terminus changed the phosphorylation sites and may have influenced downstream kinase activities.In vitro,the proliferating cell nuclear antigen indicator and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that HEK293T cells transfected with Sc-EGFR-1a and Sc-EGFR-1b exhibited different proliferation patterns.Sc-EGFR-1a caused more rapid proliferation than Sc-EGFR-1b and the EGFP-N1 control.In vivo,Sc-EGFRs were successfully knocked-down for at least 15 days by injecting the clams with the dsRNA and was associated with a significant reduction in shell length.Our results reveal for the first time in mollusks the existence of alternative splicing of EGFR and provide the basis for further studies to establish the role of EGFR in growth and development of S.constricta. | Kanyun Wei Donghong Niu Maoxiao Peng Shumei Xie Shentong Wang Jiale Li | 2018 | Aquaculture and Fisheries2018，3，2： | 0 |
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