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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | Preliminary study on light hydrocarbons in ore-forming fluids of gold and antimony deposits in southwestern Guizhou, China显示文摘METAL minerals precipitate generally from ore-forming fluid. It is suggested that organic mat-ter in ore-forming fluid is one of the best media which show the role of organic matter in oregenesis. Because mineralizing temperatures of many ore deposits exceeded the stable rangesof liquid hydrocarbons, only light hydrocarbons were preserved well in inclusions. Thus, | Zhuang, HP Lu, JL Fu, JM Liu, JZ Shi, JX | 1997 | Chinese Science Bulletin1997，42，20： | 8 |
| 3 | Gaucher disease among Chinese patients: review on genotype/phenotype correlation from 29 patients and identification of novel and rare alleles显示文摘 | Choy FY Zhang W Shi HP | 2007 | Blood Cells Mol Dis2007，38，3： | 1 |
| 4 | Altered expression and localization of the tightjunction protein ZO-1 in primary and metastatic pancreaticcancer显示文摘 | Kleeff J Shi X Bode HP | 2001 | Pancreas2001，23，： | 1 |
| 5 | CD39/CD73 and the imbalance of Th17 cells and regulatory T cells in allergic asthma显示文摘 | Wang LL Tang HP Shi GC | 2013 | Mol Med Rep2013，8，5： | 1 |
| 6 | Differential cytokine expression in skin graft healing in inducible nitric oxide synthase knockout mice显示文摘 | Most D Efron DT Shi HP | 2001 | Plast Reconstr Surg2001，108，5： | 1 |
| 7 | Altered expression and localization of the tight junction protein ZO-1 in primary and metastatic pancreatic cancer显示文摘 | Kleeff J Shi X Bode HP | 2001 | Pancreas2001，23，3： | 1 |
| 8 | Inhibition of Afiatoxin BL-DNA Binding and Adduct Formation by Selenium in Rats显示文摘 | Chua SC Lee HP | 1994 | Cancer Lett1994，82，2： | 1 |
| 9 | Expression of cyclooxygenase-2 (COX-2) in human esophageal cancer and in vitro inhibition by a specific COX-2 inhibitor, NS-398 显示文摘 | Yu HP Shi LY Lu WH | 2004 | J Gastroenterol Hepatol2004，19，6： | 1 |
| 10 | Complexity of public transport networks显示文摘 | Lu HP Shi Y | | 0，，02： | 1 |
| 11 | Hypertonic saline resuscitation limits neutrophil activation after trauma-hemorrhagic shock显示文摘 | Deitch EA Shi HP Feketeova E | 2003 | Shock2003，19，4： | 1 |
| 12 | Supplemental dietary arginine enhances wound healing in normal but not inducible nitric oxide synthase knockout mice显示文摘 | Shi HP Efron DT Most D | 2000 | Sugery2000，128，1： | 1 |
| 13 | Expression of cyclooxygenase-2 (COX-2) in human esophageal cancer and in vitro inhibition by a specific COX-2 inhibitor,NS-398显示文摘 | Yu HP Shi LY Lu WH | 2004 | J Gastroenterol Hepatol2004，19，6： | 1 |
| 14 | CD39/CD73 and the imbalance of Thl7 cells and regulatory T cells in aller- gic asthma 显示文摘 | Wang LL Tang HP Shi GC | 2013 | Mol Med Rep2013，8，5： | 1 |
| 15 | Improved biocompatibility of surface functionalized dendrimer entrapped gold nanoparticles 显示文摘 | Shi XY Wang SH Sun HP | 2007 | Soft Matter2007，3，1： | 1 |
| 16 | Draft genome of the kiwifruit Actinidia chinensis 显示文摘 | Huang SX Ding J Deng DJ Tang W Sun HH Liu DY Zhang L Niu XL Zhang X Meng M Yu JD Liu J Han Y Shi W Zhang DF Cao SQ Wei ZJ Cui YL Xia YH Zeng HP Bao K Lin L Min Y Zhang H Miao M Tang XF Zhu YZ Sui Y Li GW Sun HJ Yue JY Sun JQ Liu FF Zhou LQ Lei L Zheng XQ Liu M Huang L Song J Xu CH Li JW Ye KY Zhong SL Lu BR He GH Xiao FM Wang HL Zheng HK Fei ZJ Liu YS | 2013 | Nat Commun2013，4，： | 1 |
| 17 | Caffeic Acid Reverses Nonsteroidal Anti-inflammatory Drugs-aspirin Interaction显示文摘 | Sun SQ Hao HP Shi HB | 2011 | Chin J Nat Med2011，9，5： | 1 |
| 18 | Hypertonic saline resuscitation limits neutrophil activation after trauma hemorrhagic shock 显示文摘 | Deitch EA Shi HP Feketeova E | 2003 | Shock2003，19，4： | 1 |
| 19 | Hypertonic saline resuscitation limits neutrophil activation after traumahemorrhagic shock显示文摘 | Deitch EA Shi HP Feketeova E | 2003 | Shock2003，19，4： | 1 |
| 20 | Hypertonic saline improves intestinal mucosa barrier function and lung injury after trauma-hemorrhagic shock显示文摘 | Shi HP Peitch EA Da Xu Z | 2002 | Shock2002，17，： | 1 |
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