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| 1 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 2 | Prevalence, treatment, and associated disability of mental disorders in four provinces in China during 2001 - 05 : and epidemiological survey显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | Lancet2009，373，9680： | 1 |
| 3 | Inhibition of hypoxia- induced angiogenesis by triehostatin A via suppression of HIF-la activity in human osteosarcoma显示文摘 | Yang QC Zeng BF Shi ZM | 2006 | J Exp Clin Cancer Res2006，25，10： | 1 |
| 4 | Observation of circulating turnout cells in patients with non-small cell lung cancer by real-time fluorescent quantitative reverse transcriptase-polymerase chain reaction in peroperative period显示文摘 | Ge M J Shi D Wu QC | 2006 | J Cancer Res Clin Oncol2006，，4： | 1 |
| 5 | Prevalence,treatment,and associated disability of mental disorders in four provinces in China during 2001-05: an epidemiological survey显示文摘 | Philips PR Zhang JX Shi QC | 2009 | Lancet2009，373，9680： | 1 |
| 6 | Prevalence,treatment,and associated disability of mental disorders in four provinces in China during 2001-05:an epidemiological survey显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | Lancet2009，373，9680： | 1 |
| 7 | Prevalence, treatment, and associated disability of mental disorders in four provinces in China during 2001 - 05 : an epidemiological survey 显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | Lancet2009，373，9680： | 1 |
| 8 | Observation of circulating tumour cells in patients with non-small cell lung cancer by real-time fluorescent quantitative reverse transcriptase-polymerase chain reaction in peroperative period显示文摘 | Ge MJ Shi D Wu QC | 2006 | J Cancer Res Clin Oncol2006，132，4： | 1 |
| 9 | Prevalence,trea-tment,and associated disability of mental disorders in four provinces in China during 2001-05:an epidemiological survey显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | The Lancet2009，373，： | 1 |
| 10 | Inhibition of hypoxia-induced angiogenesis by trichostatin A via suppression of HIF-1α activity in human osteosarcoma显示文摘 | YANG QC ZENG B F SHI Z M | 2006 | J Exp Clin Cancer Res2006，25，4： | 1 |
| 11 | Prevalence,associated disability and treatment of mental disorders in four provinces in China,2001-2005:an epidemiological survey显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | Lancet2009，373，： | 1 |
| 12 | Prevalence,associated disability and treatment of mental disorders in four provinces in China,2001-2005:an epidemiological survey显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | Lancet2009，373，： | 1 |
| 13 | Prevalence, treatment, andassociated disability of mental disorders in four provinces in Chinaduring 2001 -05: an epidemiological survey 显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | Lancet2009，373，9680： | 1 |
| 14 | Observation of circulating tumour cells in patients with non-small cell lung cancer by real-time fluores- cent quantitative reverse transcriptase-polymerase chain reaction in perioperative period显示文摘 | Ge M J Shi D Wu QC | 2006 | J Cancer Res Clin Oncol2006，132，4： | 1 |
| 15 | Prevalence,treatment,and associated disability of mental disorder in four provinces in China during 2001-05:and epidemiological survey显示文摘 | Phillips MR Zhang JX Shi QC | | 0，，9680： | 1 |
| 16 | Prevalence, treatment, and associated disability of mental disorders in four provinces in China during 21301 - 05 : an epidemiological survey 显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | The Lancet2009，373，9680： | 1 |
| 17 | Prevalence, treatment, and associated disability of mental disorders in four provinces in China during 2001 -05: an epidemiological survey 显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | Lancet2009，373，9680： | 1 |
| 18 | Prevalence, treat- ment, and associated disability of mental disorders in four provinces in China during 2001-05 : and epidemiological sur- vey 显示文摘 | Phillips MR Zhang JX Shi QC etal | 2009 | Laneet2009，373，20： | 1 |
| 19 | Prevalence, treatment, and associated disability of mental disorders in four provinces in China during 2001 - 2005: An epidemiological survey 显示文摘 | Michael RP Zhang JX Shi QC | 2009 | Lancet2009，373，9680： | 1 |
| 20 | Prevalence, treatment, and associated disability of mental disorders in four provinces in China dur- ing 2001 - 05 : and epiderniolcgieal survey 显示文摘 | Phillips MR Zhang JX Shi QC | 2009 | Lancet2009，373，9680： | 1 |
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