3篇
您的检索式：作者名="Shihang Sun"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effective method for isolating liquid endosperm and improved procedure for subsequent DNA extraction in Brassica napus显示文摘 | Jun LI Shihang FAN Xingchao SUN Zhiyong HU Jing LIU Xiaoyi ZHU Ming ZHENG Fengming SUN Hongli YANG Liang ZHANG Linbin DENG Hongfang LIU Wei HUA | 2016 | Oil Crop Science2016，1，2： | 0 |
| 2 | Synthesis and characteristic of a novel green fluorescent protein eYGFPuv显示文摘Recently, a novel green fluorescent protein eYGFPuv has been identified in the marine organism Chiridius poppei which displays high fluorescence intensity and can be visible by eyes in dark. Although strong green fluorescence was achieved in transgenic petunia, 3 expression cassettes (about 8 kb) complicate its application. In this study, to confirm whether 1 expression cassette could be used as a transgenic marker in prokaryotes and eukaryotes, eYGFPuv was cloned into prokaryotic expression vector pET28α-eYGFPuv- His and plant binary expression vector 35S::eYGFPuv. Compared to EGFP, eYGFPuv protein exhibited stronger dazzling green fluorescence in E. coli under excited light at 365 nm and maintains steadily over a long period of time without degradation. When transiently expressed in tobacco leaves, eYGFPuv protein displayed strong green fluorescence. Moreover, the fluorescence of eYGFPuv protein also could be directly observed in living plant, and thus can be used easily as a marker to screen transformed lines in transgenic research. Overall, compared to previous studies on eYGFPuv tandem repeats, our data confirmed that single eYGFPuv sequence still possesses high fluorescence intensity and quenching resistance. Furthermore, because of small size of expression cassette,it is suitable for efficient transformation in both prokaryotic and eukaryotic organisms. | Shihang Fan Linbin Deng Hongli Yang Liang Zhang Xingchao Sun Jinglin Liu Jing Liu | 2019 | Oil Crop Science2019，4，2： | 0 |
| 3 | The AaCBF4-AaBAM3.1 module enhances freezing tolerance of kiwifruit (Actinidia arguta)显示文摘Beta-amylase(BAM)plays an important role in plant resistance to cold stress.However,the specific role of the BAM gene in freezing tolerance is poorly understood.In this study,we demonstrated that a cold-responsive gene module was involved in the freezing tolerance of kiwifruit.In this module,the expression of AaBAM3.1,which encodes a functional protein,was induced by cold stress.AaBAM3.1-overexpressing kiwifruit lines showed increased freezing tolerance,and the heterologous overexpression of AaBAM3.1 in Arabidopsis thaliana resulted in a similar phenotype.The results of promoter GUS activity and cis-element analyses predicted AaCBF4 to be an upstream transcription factor that could regulate AaBAM3.1 expression.Further investigation of protein-DNA interactions by using yeast one-hybrid,GUS coexpression,and dual luciferase reporter assays confirmed that AaCBF4 directly regulated AaBAM3.1 expression.In addition,the expression of both AaBAM3.1 and AaCBF4 in kiwifruit responded positively to cold stress.Hence,we conclude that the AaCBF-AaBAM module is involved in the positive regulation of the freezing tolerance of kiwifruit. | Shihang Sun Chungen Hu Xiujuan Qi Jinyong Chen Yunpeng Zhong Abid Muhammad Miaomiao Lin Jinbao Fang | 2021 | Horticulture Research2021，8，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员