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| 1 | Effects of Nogo-neutralizing antibody and neurotrophin-3 on axonal regeneration following spinal cord injury in rats显示文摘BACKGROUND: Recent studies have suggested that regeneration of the central nerve fiber following spinal cord injury occurs under specific conditions. OBJECTIVE: To study the effects of Nogo-neutralizing antibody (IN-1), in combination with neurotrophin-3 (NT-3), on axonal regeneration and motor function following spinal cord injury in the rat. DESIGN, TIME AND SETTING: A randomized, controlled, animal study combining immunohistochemistry was performed at the Laboratory of Neuroanatomy of Xiangya Medical College, and Central Laboratory of Xiangya the Third Hospital, Central South University from January 2006 to December 2007. MATERIALS: Eighteen healthy, Sprague Dawley rats were randomly divided into three groups, with six rats per group: control, IN-1, and IN-1/NT-3. Hemisectioned spinal cord injury models were established by cutting the posterior 2/3 of spinal cord, which is equivalent to the T8 level. METHODS: A polyethylene tubing was inserted through into subarachnoid cavity, equivalent to the superior margin at the T8 level. Saline, IN-1, and IN-1/NT-3 were respectively injected into control, IN-1, and IN-1/NT-3 groups, three times/day for seven consecutive days. MAIN OUTCOME MEASURES: At 2 weeks post-surgery, biotin dextran amine (10%) was injected into the right sensorimotor cortex area. At day 28 post-surgery, spinal cord tissue was prepared for frozen sections. Positive astrocytic expression was observed with glial fibrillary acidic protein (GFAP) immunohistochemical staining whose proliferation level was represented by gray value, i.e. the higher the gray value was, the less the positive cells were, and growth of positive fibers was observed with a biotin dextran amine histological reaction. Motor function was measured according to BBB scores pre-operatively, as well as at days 1, 7, 14, 21, and 28 post-operatively. RESULTS: Three rats died during experimentation. By random supplement, a total of 18 rats were included. GFAP-positive astrocytes were observed in all the three groups. In the control group, astrocytes were characterized according to active function, hyperplasia, proliferation, hypertrophy, and increasing processes as compared to IN-1 group and IN-1/IN-3 group. Astrocyte hyperplasia represented by gray value in the IN-1 group was less than the control group. Gray value of GFAP-positive products in the IN-1/IN-3 group was higher than other two groups (P < 0.05). Biotin dextran amine tracing demonstrated no corticospinal tract fiber outgrowth following spinal cord injury; the fibers were incapable of passing through the glial scar in the control group. Several fibers were distributed in the proximal scar tissue region in the IN-1 group, and the regenerated fibers were disarranged. Many nerve fibers were distributed throughout the scar tissue, and even several biotin dextran amine-positive fibers were observed at the distal end of the injured segment. Post-operative Basso, Beattie, Bresnahan scores were greater than pre-operative ones, while Basso, Beattie, Bresnahan scores in the IN-1/NT-3 group were significantly greater than the other two groups at days 14, 21, and 28 post-surgery (P < 0.05). CONCLUSION: IN-1, in combination with NT-3, promoted axonal regeneration following spinal cord injury, inhibited the colloidal effect, and enhanced the correlation between proximal and distal processes to recover motor function. The recovery effect of IN-1/NT-3 on motor function was superior that of to IN-1 alone. | Ruisen Zhan Shijie Chen Weiguo Wang Haibin Long | 2008 | Neural Regeneration Research2008，3，12： | 10 |
| 2 | OsSPL18 controls grain weight and grain number in rice显示文摘Grain weight and grain number are two important traits directly determining grain yield in rice. To date,a lot of genes related to grain weight and grain number have been identified; however, the regulatory mechanism underlying these genes remains largely unknown. In this study, we studied the biological function of OsSPL18 during grain and panicle development in rice. Knockout (KO) mutants of OsSPL18exhibited reduced grain width and thickness, panicle length and grain number, but increased tiller number. Cytological analysis showed that OsSPL18 regulates the development of spikelet hulls by affecting cell proliferation. qRT-PCR and GUS staining analyses showed that OsSPL18 was highly expressed in developing young panicles and young spikelet hulls, in agreement with its function in regulating grain and panicle development. Transcriptional activation experiments indicated that OsSPL18is a functional transcription factor with activation domains in both the N-terminus and C-terminus, and both activation domains are indispensable for its biological functions. Quantitative expression analysis showed that DEP1, a major grain number regulator, was significantly down-regulated in OsSPL18 KO lines.Both yeast one-hybrid and dual-luciferase (LUC) assays showed that OsSPL18 could bind to the DEP1promoter, suggesting that OsSPL18 regulates panicle development by positively regulating the expression of DEP1. Sequence analysis showed that OsSPL18 contains the OsmiR156k complementary sequence in the third exon; 5?RLM-RACE experiments indicated that OsSPL18 could be cleaved by OsmiR156k. Taken together, our results uncovered a new OsmiR156k-OsSPL18-DEP1 pathway regulating grain number in rice. | Hua Yuan Peng Qin Li Hu Shijie Zhan Shifu Wang Peng Gao Jing Li Mengya Jin Zhengyan Xu Qiang Gao Anping Du Bin Tu Weilan Chen Bingtian Ma Yuping Wang Shigui Li | 2019 | Journal of Genetics and Genomics2019，46，1： | 8 |
| 3 | Effects of combined application of Nogo-neutralizing antibody IN-1 and neurotrophin-3 on c-Fos and c-Jun expression in a rat model of hemisection spinal cord injury显示文摘BACKGROUND:Nogo-neutralizing antibody IN-1 accelerates axon growth and enhances recovery of spinal cord function by inhibiting growth inhibitory factors. Neurotrophin-3 (NT-3)contributes to regeneration of nerve fibers in the spinal cord and motor function recovery. The combination of Nogo-neutralizing antibody IN-1 and NT-3 is hypothesized to produce better outcomes and facilitate axonal regeneration by affecting c-Fos and c-Jun protein expression. OBJECTIVE:To investigate the combined effects of Nogo-neutralizing antibody IN-1 and NT-3 on c-Fos and c-Jun protein levels in the injured spinal cord. DESIGN,TIME AND SETTING:A randomized,controlled study was performed at the Laboratory of Neuroanatomy,Xiangya Medical College,Central South University and the Central Laboratory of Third Xiangya Hospital of China from June 2005 to December 2007. MATERIALS:NT-3 (Peprotech,USA) and Nogo-neutralizing antibody IN-1 (Santa Cruz Biotechnology,USA) were used in this study. METHODS:Hemisectioned spinal cord injury models were established by cutting the posterior 2/3 of rat spinal cord,which is equivalent to the T8 level in the human spine. A total of 120 rats were equally and randomly assigned to three groups:model (0.2 μL saline),IN-1 (0.2 μL IN-1),and IN-1/NT-3 (0.2 μL IN-1 + 0.2 μL NT-3). The compounds were separately infused into transection sites on the side of head. MAIN OUTCOME MEASURES:Western blot analysis was employed to measure c-Fos and c-Jun protein expression in the injured spinal cord at 15,30 minutes,1,2,4,6,8,and 12 hours following surgery. RESULTS:Following spinal cord injury,c-Fos and c-Jun protein expression were increased and peaked at 4-6 hours. Following injection of IN-1 or the combination of IN-1 and NT-3,c-Fos protein expression was significantly reduced in the injured spinal cord (P < 0.05 or P < 0.01) (with the exception of the 15 minute time point). However,c-Jun protein expression was significantly increased (P < 0.05 or P < 0.01) (with the exception of the 15 and 30 minute time points). Combined application of IN-1 and NT-3 resulted in significantly altered protein expression compared to IN-1 alone. CONCLUSION:IN-1 increases c-Jun protein levels and protects the injured spinal cord by inhibiting c-Fos protein levels. Moreover,the effects of IN-1 combined with NT-3 are more significant than with IN-1 alone. | Ruisen Zhan Xiongwu Long Weiguo Wang Shijie Chen Fengqi Huang | 2010 | Neural Regeneration Research2010，5，6： | 2 |
| 4 | Enhancing photovoltaic performance via aggregation dynamics control in fused-ring electron acceptor显示文摘A new fused-ring electron acceptor FNIC3 with dynamics controlled aggregation behavior was synthesized.FNIC3 shows strong absorption in 600–900 nm,HOMO/LUMO energy levels of−5.59/−4.04 eV,and electron mobility of 1.2×10^(−3) cm^(2) V^(−1) s^(−1).The aggregation of FNIC3 shows strong dependency on film formation time.Prolongation of film formation time promotes the crystallization of FNIC3,leading to improved crystallinity and enlarged aggregate sizes.Aggregation of FNIC3 significantly influences the photovoltaic device parameters.Appropriate aggregation red-shifts the absorption and improves the mobilities of the blend,which contributes to high photocurrent and fill factor thus high power conversion efficiency(PCE).Overaggregation leads to increased nonradiative energy loss and insufficient charge generation,resulting in decreased open-circuit voltage and short-circuit current density.The blends based on PM6:FNIC3 fabricated under proper film formation time exhibit a PCE of 12.3%,higher than those fabricated under short and long film formation time(10.0–10.5%). | Jiayu Wang Runyu Zhu Shijie Wang Yawen Li Boyu Jia Jiadong Zhou Peiyao Xue Susanne Seibt Yuze Lin Zengqi Xie Wei Ma Xiaowei Zhan | 2021 | Aggregate2021，2，3： | 1 |
| 5 | Creation of cylindrical vector beams through highly anisotropic scattering media with a single scalar transmission matrix calibration显示文摘Cylindrical vector(CV)beams have attracted increasing interest due to their particular properties and their applications in optical imaging,optical manipulation,and light-matter interactions.However,it is challenging to construct CV beams through highly anisotropic scattering media(HASM),such as thick biological tissue,posing a barrier to the applications of CV beams that involve HASM.Here,we present a scheme to construct CV beams beyond high scattering that only requires a single scalar transmission matrix(TM)calibration and manipulation of the spatial degrees of freedom of the scalar input field.Assisted by a radial polarization converter(S-waveplate)and a polarizer,the scheme enables one to obtain the correct incident wavefront for the creation of CV beams through HASM with only one single scalar TM calibration.Compared to the existing method,this user-friendly approach is fast and simple in terms of the optical implements and computations.Both radially and azimuthally polarized beams are experimentally constructed through a Zn O scattering layer to demonstrate the viability of the method.Arbitrarily generalized CV beams and arrays of CV beams are also created through the HASM to further prove the flexibility of the method.We believe this work may pave the way for applications of CV beams that involve a highly anisotropic scattering environment. | QIAN ZHAO SHIJIE TU QIANNAN LEI CHENGSHAN GUO QIWEN ZHAN YANGJIAN CAI | 2022 | Photonics Research2022，10，7： | 1 |
| 6 | Modelling charge transport and electro-optical characteristics of quantum dot light-emitting diodes显示文摘Quantum dot light-emitting diodes(QD-LEDs)are considered as competitive candidate for next-generation displays or lightings.Recent advances in the synthesis of core/shell quantum dots(QDs)and tailoring procedures for achieving their high quantum yield have facilitated the emergence of high-performance QD-LEDs.Meanwhile,the charge-carrier dynamics in QD-LED devices,which constitutes the remaining core research area for further improvement of QD-LEDs,is,however,poorly understood yet.Here,we propose a charge transport model in which the charge-carrier dynamics in QD-LEDs are comprehensively described by computer simulations.The charge-carrier injection is modelled by the carrier-capturing process,while the effect of electric fields at their interfaces is considered.The simulated electro-optical characteristics of QD-LEDs,such as the luminance,current density and external quantum efficiency(EQE)curves with varying voltages,show excellent agreement with experiments.Therefore,our computational method proposed here provides a useful means for designing and optimising high-performance QD-LED devices. | Sung-Min Jung Tae Hoon Lee Sang Yun Bang Soo Deok Han Dong-Wook Shin Sanghyo Lee Hyung Woo Choi Yo-Han Suh Xiang-Bing Fan Jeong-Wan Jo Shijie Zhan Jiajie Yang Chatura Samarakoon Yoonwoo Kim Luigi GOcchipinti Gehan Amaratunga Jong Min Kim | 2021 | npj Computational Materials2021，，1： | 0 |
| 7 | TCR-mimic antibody-drug conjugates targeting intracellular tumor-specific mutant antigen KRAS G12V mutation显示文摘Limited clinical application of antibody-drug conjugates(ADCs)targeting tumor associated antigens(TAAs)is usually caused by on-target off-tumor side effect.Tumor-specific mutant antigens(TSMAs)only expressed in tumor cells which are ideal targets for ADCs.In addition,intracellular somatic mutant proteins can be presented on the cell surface by human leukocyte antigen class I(HLA I)molecules forming tumor-specific peptide/HLA I complexes.KRAS G12 V mutation frequently occurred in varied cancer and was verified as a promising target for cancer therapy.In this study,we generated two TCR-mimic antibodydrug conjugates(TCRm-ADCs),2E8-MMAE and 2 A5-MMAE,targeting KRAS G12 V/HLAA*0201 complex,which mediated specific antitumor activity in vitro and in vivo without obvious toxicity.Our findings are the first time validate the strategy of TCRm-ADCs targeting intracellular TSMAs,which improves the safety of antibody-based drugs and provides novel strategy for precision medicine in cancer therapy. | Ying Shen Xiaoyue Wei Shijie Jin Yue Wu Wenbin Zhao Yingchun Xu Liqiang Pan Zhan Zhou Shuqing Chen | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，6： | 0 |
| 8 | IN-1 combined with neurotrophin-3 for axonal growth-related gene expression after spinal cord injury显示文摘A spinal cord hemisection injury model was established in rats. Treatment with IN-1 and/or neuro-trophin-3 was found to regulate the expression of growth-associated protein 43, nerve growth factor, and basic fibroblast growth factor genes in the injured spinal cord tissues; transcript levels were first increased and then decreased. Expression levels reached a peak at days 7 (growth-associated protein 43) or 14 (nerve growth factor and basic fibroblast growth factor) following spinal cord injury. Combined treatment with neurotrophin-3 and IN-1 achieved the most apparent effect on the ex-pression and recovery of motor function. These findings confirm that combined therapy with neuro-trophin-3 and IN-1 can increase expression of growth factors in the injured spinal cord tissues and promote the axonal regeneration. | Ruisen Zhan Jinbo Xu Weiguo Wang Zhiyue Li Shijie Chen Shuangxi Sun | 2011 | Neural Regeneration Research2011，6，32： | 0 |
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