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| 1 | RNAi-Mediated Silencing of ITPK Gene Reduces Phytic Acid Content,Alters Transcripts of Phytic Acid Biosynthetic Genes,and Modulates Mineral Distribution in Rice Seeds显示文摘Phytic acid is the principal storage form of phosphorus in plant seeds and an essential signalling molecule in several regulatory processes of plant development.However,it is known as an anti-nutrient compound owing to its potent chelating property.Thus,reducing the phytic acid content in crops is desirable.Studies involving regulation of MIPS and IPK1 genes to generate low phytate rice have been reported earlier.However,the functional significance of OsITPK and the effect of its down-regulation on phytic acid content and the associated pleiotropic effects on rice have not yet been investigated.In this study,tissue specific RNA interference(RNAi)-mediated down-regulation of a major ITPK homolog(OsITP5/6K-1)resulted in 46.2%decrease in phytic acid content of T2 transgenic seeds with a subsequent 3-fold enhancement in the inorganic phosphorus content.Silencing of OsITP5/6K-1 altered the transcript levels of essential phytic acid pathway genes,without significantly affecting the transcript levels of other OsITPK homologs.Furthermore,the mapping of elements through X-ray microfluorescence analysis revealed significant changes in the spatial distribution pattern and translocation of elements in low phytate seeds.Additionally,low phytate polished seeds exhibited 1.3-fold and 1.6-fold enhancement in iron and zinc content in the grain endosperm,respectively.Silencing of OsITP5/6K-1 also altered the amino acid and myo-inositol content of the transgenic seeds.Our results successfully established that RNAi-mediated silencing of OsITP5/6K-1 gene significantly reduced the phytate levels in seeds without hampering the germination potential of seeds and plant growth.The present study provided an insight into the mechanism of phytic acid biosynthesis pathway. | Aritra KARMAKAR Sananda BHATTACHARYA Shinjini SENGUPTA Nusrat ALI Sailendra Nath SARKAR Karabi DATTA Swapan K.DATTA | 2020 | Rice science2020，27，4： | 7 |
| 2 | Rapid improvement in post-infectious gastroparesis symptoms with mirtazapine显示文摘We report the case of a 34-year-old woman with severe post-infectious gastroparesis who was transferred from an outside medical facility for a second opinion regarding management.This patient had no prior history of gastrointestinal symptoms.However,in the aftermath of a viral illness,she developed two months of intractable nausea,vomiting,and oral intake intolerance that resulted in numerous hospitalizations for dehydration and electrolyte disturbances.A solid-phase gastric emptying scan had confirmed delayed emptying,confirming gastroparesis.Unfortunately,conventional pro-kinetic agents and numerous anti-emetic drugs provided little or no relief of the patient’s symptoms.At our institution,the patient experienced a cessation of vomiting,reported a significant reduction in nausea,and toler-ated oral intake shortly after taking mirtazapine.Based on mirtazapine’s primary action as a serotonin(5-HT)1a receptor agonist,we infer that this receptor system mediated the clinical improvement through a combination of peripheral and central neural mechanisms.This report highlights the potential utility of 5-HT1a agonists in the management of nausea and vomiting.We conclude that mirtazapine may be effective in treating symptoms associated with non-diabetic gastroparesis that are refractory to conventional therapies. | Shinjini Kundu Shari Rogal Abdulkader Alam David J Levinthal | 2014 | World Journal of Gastroenterology2014，20，21： | 3 |
| 3 | Typhoid and paratyphoid fever 显示文摘 | BHAN M K RAJIV B SHinJINI B | 2005 | Lancet2005，366，9487： | 1 |
| 4 | Spatial patterns of microbial diversity and activity in an agedcreosote- contaminated site显示文摘 | SHINJINI M HELI J PAULI S | 2014 | ISM E Journal2014，151，8： | 1 |
| 5 | Typhoid and paratyphoid fever 显示文摘 | BHAN M K RAJIV B SHINJINI B | 2005 | Lancet2005，366，9487： | 1 |
| 6 | Typhoid and paratyphoid fever显示文摘 | BHAN M K RAJIV B SHINJINI B | 2005 | Lancet2005，366，9487： | 1 |
| 7 | Optimal threshold in CT quantification of emphysema显示文摘 | Zhimin Wang Suicheng Gu Joseph K. Leader Shinjini Kundu John R. Tedrow Frank C. Sciurba David Gur Jill M. Siegfried Jiantao Pu | 2013 | European Radiology2013，，4： | 1 |
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