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3篇 您的检索式:作者名="Shixiang Bao"
    题名 作者 年代 出处 被引量
1Applying terahertz time-domain spectroscopy to probe the evolution of kerogen in close pyrolysis systems显示文摘Terahertz time-domain spectroscopy (THz-TDS) has been used to probe the evolutionary paths of kerogen in selected black mudstone. The evolutionary regime of kerogens (for instance, the immaturity, early maturity, middle maturity, late maturity, and catagenesis stages) can be indicated by the absorption coefficient in the THz region. The present study of identification based on THz-TDS was in good agreement with programmed pyrolysis experiments and suggests that THz technology can act as a nondestructive, contact-free tool for probing the ability to generate hydrocarbons from kerogens.BAO RiMa WU ShiXiang ZHAO Kun ZHENG LunJu XU ChangHong 2013Science China(Physics,Mechanics & Astronomy)2013,56,8:19
2Qualitative identification of crude oils from different oil fields using terahertz time-domain spectroscopy显示文摘Honglei Zhan Shixiang Wu Rima Bao Lina Ge Kun Zhao 2015Fuel2015,,:1
3Construction and identification of recombinant plasmid pUIS3-BLC^+显示文摘Objective:To construct and identify recombinant plasmid pUIS3-BLC^+.Methods:The cDNA of B-lymphocyte chemoattractant(BLC) was amplified from the total RNA of spleen tissues by PCR method,and were inserted into plasmid of Plasmodium berghei with U1S3 knockout by digestion of restrictive endonuclease and T7 ligation.The recombinant plasmids were screened,and then underwent restriction enzymatic digestion and DNA sequencing.Then the confirmed plasmid was further transfected into COS-1 cells by lipofectamine and the BLC expression was tested by RT-PCR and Western blotting.Results:The cDNA of BLC gene was correctively amplified by RT-PCR and the recombinant plasmid pUIS3-BLC^+ was constructed successfully,which was confirmed by restriction enzymatic digestion and DNA sequencing.RT-PCR and Western blotting also showed the BLC gene expression in COS-1 cells.Conclusions:The recombinant plasmid pUIS3-BLC^+ has BLC expression in COS-1 cells,and is useful for further study on BLC transgene and UIS3 gene knockout in Plasmodium berghei.Yingzi Lin Shixiang Bao Guanghong Tan Fengying Huang Huiqin Huang 2010Asian Pacific Journal of Tropical Medicine2010,3,10:0
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