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| 1 | Production of recombinant human lactoferrin in the allantoic fluid of embryonated chick eggs and its characteristics显示文摘 | Tutykhina I L Bezborodova O A Shmarov M M | 2009 | Protein Expression Purification2009，65，： | 1 |
| 2 | Molecular and cellular changes in the post-traumatic spinal cord remodeling after autoinfusion of a genetically-enriched leucoconcentrate in a mini-pig model显示文摘Post-traumatic spinal cord remodeling includes both degenerating and regenerating processes,which affect the potency of the functional recovery after spinal cord injury(SCI).Gene therapy for spinal cord injury is proposed as a promising therapeutic strategy to induce positive changes in remodeling of the affected neural tissue.In our previous studies for delivering the therapeutic genes at the site of spinal cord injury,we developed a new approach using an autologous leucoconcentrate transduced ex vivo with chimeric adenoviruses(Ad5/35)carrying recombinant cDNA.In the present study,the efficacy of the intravenous infusion of an autologous genetically-enriched leucoconcentrate simultaneously producing recombinant vascular endothelial growth factor(VEGF),glial cell line-derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)was evaluated with regard to the molecular and cellular changes in remodeling of the spinal cord tissue at the site of damage in a model of mini-pigs with moderate spinal cord injury.Experimental animals were randomly divided into two groups of 4 pigs each:the therapeutic(infused with the leucoconcentrate simultaneously transduced with a combination of the three chimeric adenoviral vectors Ad5/35‐VEGF165,Ad5/35‐GDNF,and Ad5/35‐NCAM1)and control groups(infused with intact leucoconcentrate).The morphometric and immunofluorescence analysis of the spinal cord regeneration in the rostral and caudal segments according to the epicenter of the injury in the treated animals compared to the control mini-pigs showed:(1)higher sparing of the grey matter and increased survivability of the spinal cord cells(lower number of Caspase-3-positive cells and decreased expression of Hsp27);(2)recovery of synaptophysin expression;(3)prevention of astrogliosis(lower area of glial fibrillary acidic protein-positive astrocytes and ionized calcium binding adaptor molecule 1-positive microglial cells);(4)higher growth rates of regeneratingβIII-tubulin-positive axons accompanied by a higher number of oligodendrocyte transcription factor 2-positive oligodendroglial cells in the lateral corticospinal tract region.These results revealed the efficacy of intravenous infusion of the autologous genetically-enriched leucoconcentrate producing recombinant VEGF,GDNF,and NCAM in the acute phase of spinal cord injury on the positive changes in the post-traumatic remodeling nervous tissue at the site of direct injury.Our data provide a solid platform for a new ex vivo gene therapy for spinal cord injury and will facilitate further translation of regenerative therapies in clinical neurology. | Maria Aleksandrovna Davleeva Ravil Rasimovich Garifulin Farid Vagizovich Bashirov Andrei Aleksandrovich Izmailov Leniz Faritovich Nurullin Ilnur Ildusovich Salafutdinov Dilara Zilbarovna Gatina Dmitrij Nikolaevich Shcherbinin Andrei Aleksandrovich Lysenko Irina Leonidovna Tutykhina Maksim Mikhailovich Shmarov Rustem Robertovich Islamov | 2023 | Neural Regeneration Research2023，18，7： | 1 |
| 3 | Production of recombinant human lactoferrin in the allantoic fluid of embryonated chick eggs and its characteristics显示文摘 | Tutykhina IL Bezborodova OA Shmarov MM | 2009 | Protein Expr Purif2009，65，： | 1 |
| 4 | Immunization with influenza A NP-expressing vaccinia virus recombinant protects mice against experimental infection with human and avian influenza viruses显示文摘 | A. D. Altstein A. K. Gitelman Y. A. Smirnov L. M. Piskareva L. G. Zakharova G. V. Pashvykina M. M. Shmarov O. P. Zhirnov N. P. Varich P. O. Ilyinskii A. M. Shneider | 2006 | Archives of Virology2006，，5： | 1 |
| 5 | Combined Stimulation of Toll-Like Receptor 5 and NOD1 Strongly Potentiates Activity of NF-κB, Resulting in Enhanced Innate Immune Reactions and Resistance to Salmonella enterica Serovar Typhimurium Infection显示文摘 | Amir I. Tukhvatulin Ilya I. Gitlin Dmitry V. Shcheblyakov Natalia M. Artemicheva Lyudmila G. Burdelya Maxim M. Shmarov Boris S. Naroditsky Andrei V. Gudkov Alexander L. Gintsburg Denis Y. Logunov | 2013 | Infection and Immunity2013，，10： | 1 |
| 6 | The induction o{ peotective immune response in mince vaccinated by recombi- nant avian adenovirus CELO expressing glycoprotein G of the rabies virus 显示文摘 | Shmarov M M Tutykhina I L Diu L | 2006 | Zh mikrobiol Epidomial Immunobiol2006，，4： | 1 |
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