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| 1 | Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability显示文摘The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation.Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system.In this study,twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection,regeneration capacity,and stable transgenic efficiency.Three genotypes,Yuechun 04-5,Yuechun 03-3,and Tianlong 1,showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system.For the Tianlong 1,the average stable transformation efficiency is 4.59%,higher than that of control genotypes(Jack and Williams 82),which is enough for further genomic research and genetic engineering.While polymerase chain reaction(PCR),LibertyLink strips,and β-glucuronidase(GUS) staining assays were used to detect the insertion and expression of the transgene,leaves painted with 135 mg/L Basta could efficiently identify the transformants. | Zhang-yue SONG Jing-luan TIAN Wei-zhe FU Lin LI Ling-hong LU Lian ZHOU Zhi-hui SHAN Gui-xiang TANG Hui-xia SHOU | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，4： | 17 |
| 2 | Vagus efect on pylorus-preserving gastrectomy显示文摘INTRODUCTIONMakiandothersreportedin1967thestudyofapyloruspreservinggastrectomy(PPG)[1].Itsuccesfulypreventeddumpingsyndromea... | LU Yun Fu, ZHAO Ge, GUO Cui Ying, JIA Shou Ren and HOU Yi Ding | 1999 | World Journal of Gastroenterology1999，5，2： | 5 |
| 3 | Recent progress of laser driven particle acceleration at Peking University显示文摘最近,放射压力加速(RPA ) 被建议了并且广泛地学习了,它证明那循环地极化了(CP ) 激光脉搏能在 phase-stable-acceleration (PSA ) 加速单音精力充沛的离子捆从 ultrathin 陪衬的方法。自我组织的质子横梁能稳定地在 10 点在和一个平面目标的 CP 激光的相互作用被加速到 GeV,这被发现 < 啜 class= “ a-plus-plus ” > 22 W/cm < 啜 class= “ a-plus-plus ” > 2 。称为紧缩的激光血浆质子加速器(CLAPA ) 的一个工程最近被大多数在中国同意。激光的一个原型驾驶质子加速器(1 ~ 15 MeV/1 Hz ) 将基于 PSA 机制和血浆透镜在下一五年里在北京大学被造。它以后将为惯性的范围熔化为象癌症治疗,血浆成像和快点火那样的应用被升级到 200 兆电子伏。 | Xue-Qing Yan Chen Lin Hai-Yang Lu Kun Zhu Yu-Bin Zou Hong-Yong Wang Bing Liu Shuan Zhao Jiao Zhu Yi-Xing Geng, He-Zheng Fu Yong Shang Chao Cao Yin-Ren Shou Wei Song Yuan-Rong Lu Zhong-Xi Yuan Zhi-Yu Guo Xian-Tu He Jia-Er Chen | 2013 | Frontiers of physics2013，8，5： | 3 |
| 4 | An Efficient One-step Methyl Esterification of Carboxylic Acid and Deacetylation of Alcohol under BF_3·OEt_2-MeOH显示文摘An efficient one-step methyl esterification of carboxylic acid and deacetylation of alcohol under BF3O·Et2-MeOH was developed. | Shou Fu LU Qin Qin OUYANG Zhong Wu GUO Biao YU and Yong Zheng HUI(State Key Laboratory of Bioorganic and Natural Products Chemistry,Shanghai Institute of Organic Chemistry, Academia Sinica, Shanghal 200032) | 1997 | Chinese Chemical Letters1997，8，10： | 1 |
| 5 | A New Method for the Cleavage of Carbohydrate Benzylidene Acetal by Ceric (IV) Ammonium Nitrate in CH_3CN-H_2O (10: 1)显示文摘A new method for the cleavage of carbohydrate benzylidene acetal has been developed using Ceric (YV) ammonium nitrate (CAN) [(NH4)2Ce(NO3)6] in CH3CN-H2O (10/1, v/v). | Shou Fu LU Qin Qin OUYANG Zhong Wu GUO Biao YU and Yong Zheng HUI(State Key Laboratory of Bio-organic and Natural Products Chemistry,Shanghai Institute of Organic Chemistry, Academia Sinica, Shanghai 200032) | 1997 | Chinese Chemical Letters1997，8，10： | 0 |
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