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| 1 | Applications and potentials of nanopore sequencing in the(epi)genome and(epi)transcriptome era显示文摘The Human Genome Project opened an era of(epi)genomic research,and also provided a platform for the development of new sequencing technologies.During and after the project,several sequencing technologies continue to dominate nucleic acid sequencing markets.Currently,Illumina(short-read),PacBio(long-read),and Oxford Nanopore(longread)are the most popular sequencing technologies.Unlike PacBio or the popular short-read sequencers before it,which,as examples of the second or so-called Next-Generation Sequencing platforms,need to synthesize when sequencing,nanopore technology directly sequences native DNA and RNA molecules.Nanopore sequencing,therefore,avoids converting mRNA into cDNA molecules,which not only allows for the sequencing of extremely long native DNA and full-length RNA molecules but also document modifications that have been made to those native DNA or RNA bases.In this review on direct DNA sequencing and direct RNA sequencing using Oxford Nanopore technology,we focus on their development and application achievements,discussing their challenges and future perspective.We also address the problems researchers may encounter applying these approaches in their research topics,and how to resolve them. | Shangqian Xie Amy Wing-Sze Leung Zhenxian Zheng Dake Zhang Chuanle Xiao Ruibang Luo Ming Luo Shoudong Zhang | 2021 | The Innovation2021，2，4： | 2 |
| 2 | Corrosion behaviour of sintered NdFeB coated with Al/Al 2 O 3 multilayers by magnetron sputtering显示文摘 | Shoudong Mao Hengxiu Yang Feng Huang Tingting Xie Zhenlun Song | 2010 | Applied Surface Science2010，，9： | 1 |
| 3 | Hydrologic implications of the isotopic kinetic fractionation of open-water evaporation显示文摘The kinetic fractionation of open-water evaporation against the stable water isotope H_2 ^(18)O is an important mechanism underlying many hydrologic studies that use ^(18)O as an isotopic tracer. A recent in-situ measurement of the isotopic water vapor flux over a lake indicates that the kinetic effect is much weaker(kinetic factor 6.2‰) than assumed previously(kinetic factor14.2‰) by lake isotopic budget studies. This study investigates the implications of the weak kinetic effect for studies of deuterium excess-humidity relationships, regional moisture recycling, and global evapotranspiration partitioning. The results indicate that the low kinetic factor is consistent with the deuterium excess-humidity relationships observed over open oceans.The moisture recycling rate in the Great Lakes region derived from the isotopic tracer method with the low kinetic factor is a much better agreement with those from atmospheric modeling studies than if the default kinetic factor of 14.2‰ is used. The ratio of transpiration to evapotranspiration at global scale decreases from 84±9%(with the default kinetic factor) to 76±19%(with the low kinetic factor), the latter of which is in slightly better agreement with other non-isotopic partitioning results. | Wei XIAO Yufei QIAN Xuhui LEE Wei WANG Mi ZHANG Xuefa WEN Shoudong LIU Yongbo HU Chengyu XIE Zhen ZHANG Xuesong ZHANG Xiaoyan ZHAO Fucun ZHANG | 2018 | Science China Earth Sciences2018，61，10： | 1 |
| 4 | Facile Synthesis of S-Fused Multi-Membered Polycyclic Heterocycles:A Construction Strategy towards Thermally Stable Thiepine Derivatives显示文摘S-fused heterocycles have become popular building blocks to construct functional polycyclic compounds.In contrast to the abundant synthetic methodologies for thiophene-fused aromatics,the synthesis of S-heterocycles containing six-membered thiopyran and seven-membered thiepine rings is much less reported owing to their unfavorable synthetic protocols and the thermal instabilities.Herein,a series of thiepine-containing polycyclic S-heterocycles have been successfully synthesized via different synthetic routes which involve initial construction of sulfur bridges and final ring-closure reactions.Therefore,the dilithium intermediates are ex-cluded,which facilitates the fusion on the thiepine ring with different S-heterocycles,including thiophene and thiopyran derivatives.Typically,a S-fused multi-membered polycyclic compound simultaneously involving thiophen,thiopyran,and thiepine rings has been successfully prepared.Interestingly,nucleus-independent chemical shift calculations reveal that the incorporated thiopyran and thiepine rings demonstrate aromatic and nonaromatic characteristics,respectively.Moreover,the thermal stabilities of the thiepine derivatives have been tremendously improved after the fusion on the three vinyl groups in the thiepine unit,which is attributed to the enhancements of the activation energies for the S-extrusion reactions,as revealed by density functional theory calculations.Therefore,our findings not only provide a facile synthetic methodology for S-fused multi-membered polycyclic heterocycles,but also furnish a novel construction strategy towards thermally stable thiepine derivatives. | Binbin Ming Chuan Yan Shoudong Xie Si Liu Yingjian Ren Hao Zong Weinan Chen Gang Zhou | 2023 | Chinese Journal of Chemistry2023，41，1： | 0 |
| 5 | Facile synthesis and functionalization of fluoranthenes via intramolecular[4+2]annulations between thiophenes and alkynes显示文摘Fluoranthenes have attracted tremendous attention due to their unique optoelectronic properties and extensive applications.Although several synthetic methodologies have been developed for the preparation of fluoranthene derivatives,it is still unfavorable to functionalize the fluoranthene framework at different positions due to the relatively low selectivity and reactivity.Herein,a catalyst-free intramolecular[4+2]annulation between thiophenes and alkynes is developed towards the synthesis of fluoranthenes.Altogether 20 examples have been demonstrated using this method.Various functional groups can be precisely introduced into the fluoranthene skeleton at different positions by simply tuning the substituents on the thiophenes and alkynes.The conjugation of the fluoranthene can be facilely extended through different directions.Furthermore,the feasibility of this[4+2]annulation reaction is also investigated by density functional theory calculations.Therefore,this protocol provides not only a synthetic methodology towards fluoranthenes with substituents functionalized at different positions,but also an effective pathway to construct large polycyclic aromatic hydrocarbons containing fluoranthene moieties. | Shoudong Xie Weinan Chen Si Liu Hao Zong Binbin Ming Gang Zhou | 2023 | Chinese Chemical Letters2023，34，6： | 0 |
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