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| 1 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 2 | Design and experimental performance verification of a thermal property test-bed for lunar drilling exploration显示文摘Chinese Chang'e lunar exploration project aims to collect and return subsurface lunar soil samples at a minimum penetration depth of 2 m in 2017. However, in contrast to those on the Earth, automated drilling and sampling missions on the Moon raise the risk of burning bits.Test-beds are required for testing the thermal properties of drill tools in a lunar environment. In this paper, a novel temperature measuring method based on thermocouples and a slip ring was proposed. Furthermore, a data acquisition system for a drilling process was designed. A vacuous,cryogenic, and anhydrous soil environment simulating the lunar surface was established. A drilling test-bed that can reach a depth of 2.2 m was developed. A control strategy based on online monitoring signals was proposed to improve the drilling performance. Vacuum and non-vacuum experiments were performed to test the temperature rising effect on drill tools. When compared with the non-vacuum experiment, the vacuum temperature rise resulted in a 12 °C increase. These experimental results provide significant support for Chinese lunar exploration missions. | Zhang Tao Zhao Zeng Liu Shuting Li Jinglin Ding Xilun Yin Shen Wang Guoxin Lai Xiaoming | 2016 | Chinese Journal of Aeronautics2016，29，5： | 3 |
| 3 | A Strategy for Searching Antigenic Regions in the SARS-CoV Spike Protein显示文摘In the face of the worldwide threat of severe acute respiratory syndrome (SARS)to human life, some of the most urgent challenges are to develop fast and accurate analytical methods for early diagnosis of this disease as well as to create a safe anti-viral vaccine for prevention. To these ends, we investigated the antigenicity of the spike protein (S protein), a major structural protein in the SARS-coronavirus (SARS-CoV). Based upon the theoretical analysis for hydrophobicity of the S protein, 18 peptides were synthesized. Using Enzyme-Linked Immunosorbent Assay (ELISA), these peptides were screened in the sera from SARS patients. According to these results, two fragments of the S gene were amplified by PCR and cloned into pET-32a. Both S fragments were expressed in the BL-21 strain and further purified with an affinity chromatography. These recombinant S fragments were confirmed to have positive cross-reactions with SARS sera, either by Western blot or by ELISA. Our results demonstrated that the potential epitope regions were located at Codons 469-882 in the S protein, and one epitope site was located at Codons 599-620. Identification of antigenic regions in the SARS-CoV S protein may be important for the functional studies of this virus or the development of clinical diagnosis. | Yan Ren, Zhengfeng Zhou, Jinxiu Liu, Liang Lin, Shuting Li, Hao Wang, Ji Xia, Zhe Zhao, Jie Wen, Cuiqi Zhou, Jingqiang Wang, Jianning Yin, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China. | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 1 |
| 4 | A functional PVA aerogel-based membrane obtaining sutureability through modified electrospinning technology and achieving promising anti-adhesion effect after cardiac surgery显示文摘Pericardial barrier destruction,inflammatory cell infiltration,and fibrous tissue hyperplasia,trigger adhesions after cardiac surgery.There are few anti-adhesion materials that are both functional and sutureable for pericardial reconstruction.Besides,a few studies have reported on the mechanism of preventing pericardial adhesion.Herein,a functional barrier membrane with sutureability was developed via a modified electrospinning method.It was composed of poly(L-lactide-co-caprolactone)(PLCL)nanofibers,poly(vinyl alcohol)(PVA)aerogel,and melatonin,named PPMT.The PPMT had a special microstructure manifested as a staggered arrangement of nanofibers on the surface and a layered macroporous aerogel structure in a cross-section.Besides providing the porosity and hydrophilicity obtained from PVA,the structure also had suitable mechanical properties for stitching due to the addition of PLCL nanofibers.Furthermore,it inhibited the proliferation of fibroblasts by suppressing the activation of Fas and P53,and achieved anti-inflammatory effects by affecting the activity of inflammatory cells and reducing the release of pro-inflammatory factors,such as interleukin 8(IL-8)and tumor necrosis factorα(TNF-α).Finally,in vivo transplantation showed that it up-regulated the expression of matrix metalloproteinase-1(MMP1)and tissue inhibitor of metalloproteinase-1(TIMP1),and down-regulated the expression of Vinculin and transforming growth factorβ(TGF-β)in the myocardium,thereby reducing the formation of adhesions.Collectively,these results demonstrate a great potential of PPMT membrane for practical application to anti-adhesion. | Dawei Jin Shuofei Yang Shuting Wu Meng Yin Haizhu Kuang | 2022 | Bioactive Materials2022，7，4： | 1 |
| 5 | Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice显示文摘Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products. | Shuting Yin Qiangqiang Li Yuxiao Tao Enning Zhou Kun Wang Wanwen Chen Xiangxin Li Liming Wu | 2024 | Food Science and Human Wellness2024，13，1： | 0 |
| 6 | Copper-linked IT MoS2/Cu2O Heterostructure for Efficient Photocatalytic Hydrogen Evolution显示文摘Metallic IT phase molybdenum disulfide(lT MoS2)is an excellent catalyst due to the abundant active sites and good conductivity.However,complex synthesis and unstable nature of IT MoS2 still limit its practical application.Herein,we propose a simple method to trigger the phase transition of MoS2.This phase transition is first predicted by density functional theory(DFT)calculations and then confirmed by Raman spectroscopy and high-resolution transmission electron microscopy(HRTEM).IT MoS2/CU2O heterostructure catalyst is then developed,showing enhanced photocatalytic hydrogen evolution. | YIN Yage WEI Shuting ZHANG Lei GUO Ziwang HUANG Haihua SAl Shiran WU Jiandong XU Yanchao LIU Ying ZHENG Lirong FAN Xiaofeng CUI Xiaogiang | 2020 | Chemical Research in Chinese Universities2020，36，6： | 0 |
| 7 | Vertical graphene-coated Cu wire for enhanced tolerance to high current density in power transmission显示文摘Cu wires(CuWs)are widely used as electric transmission lines.However,their limited thermal and chemical stabilities become challenges under the high-power and harsh environment.Graphene is regarded as an ideal protective barrier for CuW benefiting from its impermeability to all atoms and molecules.Particularly,the excellent hydrophobicity of vertical graphene(VG)will strengthen its protective capability as a corrosion and oxidation barrier.Herein,VG is directly synthesized on CuW by plasmaenhanced chemical vapor deposition method.The hydrophobic VG coating with a high water contact angle can effectively exclude the corrosive liquid and moisture from CuW surface and prevent their further penetration.Consequently,the electrochemical corrosion rate of VG-CuW is reduced by~13,8,and 2 times,compared with bare CuW,VG-CuW with hydrophilic treatment,and CuW coated with thick horizontal graphene layers,respectively.Negligible oxidation occurs on VGCuW after the long-time exposure to humid air at~200℃ along with the largely enhanced tolerance under high-current operating condition.This study reveals the impressive potentials of hydrophobic VG as a robust corrosion and oxidation barrier for metal wires used in high-power cables and electronic devices in harsh environment. | Kun Wang Shuting Cheng Qingmei Hu Feng Yu Yi Cheng Kewen Huang Hao Yuan Jun Jiang Wenjuan Li Junliang Li Shichen Xu Jianbo Yin Yue Qi Zhongfan Liu | 2022 | Nano Research2022，15，11： | 0 |
| 8 | Solid State Reaction Preparation of LiFePO_4/(C+Cu) Cathode Material and Its Electrochemical Performance显示文摘CueC co-coated LiFePO4(LiFePO4/(C+Cu)) cathode material was successfully prepared through solid state reduction reaction.The optimized additive amount of℃uO was determined by electrochemical test of series content-dependent samples.Electrochemical performances of LiFePO4/(C+Cu) cathode material were investigated.℃rystalline structure,morphology and electrochemical performance of the samples were characterized by X-ray diffraction,scanning electron microscopy(SEM),transmission electron microscopy(TEM),energy dispersive spectroscopy(EDS),chargeedischarge tests and AC impedance techniques.Results showed that crystal structure of the bulk material was not destroyed after℃u particles distributed on the surface of LiFePO4/C.With 5 wt%℃uO additive,the LiFePO4/(C+Cu) cathode material showed improved electrochemical performance especially at high rates and low temperature.At 25℃ and 0.1℃ current rate,specific capacity of the℃u-coated sample reaches 161.3 mA h/g.The result was 47 mA h/g higher than that of the un-coated one.At 20℃,the discharge capacity of℃u-coated materials was 113.4 mA h/g at 0.1℃ rate and 83.8 mA h/g at 5℃ rate,which reached about 70% of that at room temperature,respectively. | Yanhong Yin Xiangnan Li Xinxin Mao Xianliang Ding Shuting Yang | 2013 | Journal of Materials Science & Technology2013，29，10： | 0 |
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