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| 1 | Extraction and purification of TGFβ and its effect on the induction of apoptosis of hepatocytes显示文摘AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine platelets by acid/ethanol extraction method and purified with ion exchange and gel chromatography. The extracted TGF β was injected subcutaneously to mice, and its biological activity in vivo was observed 72 hfs post-injection by HE staining. The morphological changes were observed by HE staining and the occurrence of apoptosis was detected by TUNEL method after the human normal hepatic cell line QZG was treated with 8 μg@L 1 TGFβ for 12 hrs in vitro.RESULTS The molecular mass 25 ku TGF β protein was successfully extracted. It was able to induce localized granulation tissue formation in vivo. TGF β-treated hepatocytes showed obvious apoptotic morphological changes, including the pyknosis and dense-stained nuclei and cytoplasm, the fragmentary, annular or crescent nuclei, and the 'bubbling' cytoplasm. Moreover, its apoptotic rate was significantly higher than that of the control group (P<0.05).CONCLUSION Biological active TGF β protein is extracted and purified successfully from bovine platelets, and it is able to induce the apoptosis of hepatocytes. | Xiao-Hui Si Lian-Jun Yang Research Institute of Stomatology,the Ninth People’s Hospital,Shanghai Second Medical University,Shanghai 200011,ChinaDepartment of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 15 |
| 2 | 4例虹膜蔓状血管瘤光学相干体层成像术血管造影特征显示文摘血管畸形中失调的TGF-β/BMP信号通路
血管树正确的结构要求调控管腔形成,血管生长和重塑的信号通路激活并达到平衡。当这一平衡被打破时,会出现血管发育畸形。该文旨在回顾转化生长因子(TGF)-β、骨形成蛋白(BMP)信号通路在血管形成和发展中的作用。胚胎期灭活这些信号通路,将导致血管组织变异。 | Chien JL Sioufi K Ferenczy S Say EAT 张凌 Cunha SI Magnusson PU Dejana E | 2017 | 中国口腔颌面外科杂志2017，15，6： | 6 |
| 3 | Influence of Fe Addition on Products of Self-Propagating High-Temperature Synthesis Reaction in 3Ti-Si-2C System显示文摘Influence of Fe addition on products of self-propagating high-temperature synthesis (SHS) reaction in 3Ti-Si-2C system was investigated in the present study. Without Fe addition, Ti5Si3 and TiC are the dominant phases along with a small amount of Ti3SiC2 phase and unreacted C left in the final products. As Fe content ranges from 10% to 30%, the products consist of TiC, Ti5Si3, Fe2Ti and unreacted C, but no trace of Ti3SiC2 phase is detected. Furthermore, the amounts of both Fe2Ti and C phases increase with Fe content increasing. Addition of Fe has a great effect on the reaction route and significantly restrains the formation of Ti3SiC2 during the combustion synthesis process, and therefore, the SHS is not an effective fabrication technique to synthesize the ternary Ti3SiC2 ceramic in either 3Ti-Si-2C or Fe-3Ti-Si-2C system. Besides, without Fe addition, Ti5Si3 presents as the coarse irregular appearance with an obviously sintered morphology. In contrast, the shape of Ti5Si3 exhibits more and more spherical (cobblestone-like) and the surface becomes increasingly smooth, because the amount of liquids formed during the SHS reaction increases with the increase of Fe content. On the other hand, with Fe content increasing from 0 to 30 wt.%, the particulate size of TiC decreases from more than 5 μm to 1 μm or less, mainly due to the fact that the combustion temperature decreases with the increase of Fe content in the preforms. | Guan Q L Wang H Y Li S L Si W P Jiang Q C | 2009 | 稀有金属材料与工程2009，38，A03： | 2 |
| 4 | 定期运动有助于改善高血压患者蛋白质稳态、炎症和血管活性显示文摘体育锻炼是一种公认的非药物控制血压的策略,除了降低血压外,运动还能对高血压患者的炎症特征、自主功能、内皮功能和抗氧化状态产生积极的影响。但其对高血压患者的蛋白质稳态的影响尚不清楚。已有动物研究表明,运动训练干预可以改善蛋白质质量控制机制,但在动物模型中报告的积极作用仍有待于在人类身上得到证实。本研究旨在根据报告的运动习惯,评估患高血压成年人的蛋白质稳态,生活质量以及炎症、氧化应激和血管活性生物标记物状态。 | Teixeira M Gouveia M Duarte A Ferreira M Simões MI Conceição M Silva G Magalhães S Ferreira R Nunes A Vieira SI Ribeiro F 周卫(译) 叶鹏(审校) | 2020 | 中华高血压杂志2020，28，12： | 2 |
| 5 | Antipsychotic Medications in Major Depression and the Association with Treatment Satisfaction and Quality of Life: Findings of Three National Surveys on Use of Psychotropics in China Between 2002 and 2012显示文摘 | Yu-Xi Wang Yu-Tao Xiang Yun-Ai Su Qian Li Liang Shu Chee H Ng Gabor S Ungvari Helen FK Chiu Yu-Ping Nin Gao-Hua Wang Pei-Shen Bai Tao Li Li-Zhong Sun Jian-Guo Shi Xian-Sheng Chen Qi-Yi Mei Ke-Qing Li Xin Yu Tian-Mei Si | 2015 | Chinese Medical Journal2015，，14： | 2 |
| 6 | Microvessel quantition and its possible relation with metastasis in colorectal cancer 显示文摘 | Tomisaki SI Ohni S | 1996 | Cancer1996，，： | 2 |
| 7 | Near-normoglycaemic remission in African-Americans with Type 2 diabetes mellitus is associated with recovery of beta cell function显示文摘 | McFarlane SI Chaiken RL Hirsch S | 2001 | Diabet Med2001，18，1： | 1 |
| 8 | Near-infrared spectroscopy: a potential method for continuous, transcutaneous monitoring for compartment syndrome in critically injured patients 显示文摘 | Arbabi S Brundage SI Gentilello LM | 1999 | J Trauma1999，47，： | 1 |
| 9 | miR-21-mediated tumor growth显示文摘 | Si ML Zhu S WuH | 2007 | Oncogene2007，26，19： | 1 |
| 10 | Effect of Hook Subunit Concentration on Assembly and Control of Length of the Flagellar Hook of Salmonella 显示文摘 | Muramoto K Makishima S Aizawa Si | 1999 | J Bacteriol1999，181，18： | 1 |
| 11 | Effect of perforated ratios of distributor on the fluidization characteristics in a gas-solid fluidized bed显示文摘 | Dong S Q Cao C Q Si C D etal | 2009 | Ind Eng Chem Res2009，48，1： | 1 |
| 12 | RP-LC simultaneous de- termination of nebivolol hydrochloride and amlodipine be- silate in bi-layer tablets显示文摘 | Joshi S J Karbhari PA Bhoir SI | 2009 | Chromatographia2009，70，34： | 1 |
| 13 | N-acyl-bomoserine lactone ptake and systemic transport in barle yrest upon active parts of the plant显示文摘 | Si Eper T F K S | 2014 | New Phytol2014，，201： | 1 |
| 14 | Sorbitol dehydrogenase over-expression potentiates glucose toxicity to cultured retinal pericytes显示文摘 | Amano S Yamagishi SI Kato N Inagaku Y Okamoto T Makino M | 2002 | Bio Biophy Res Commun2002，299，2： | 1 |
| 15 | A highly selective photoelectrochemical biosensor for uric acid based on core-shell Fe O@C nanoparticle and molecularly imprinted Ti O显示文摘 | Zhang C Si S Yang Z | 2014 | Biosensors and Bioelectronics2014，65，： | 1 |
| 16 | Can children w ith autism master the core deficit and become empathetic,creative,and reflective?A ten to fifteen year follow-up of a subgroup of children with autism spectrum disorders(ASD)who received a comprehensive developmental,individual-difference,relationship-based(DIR)approach显示文摘 | Wielder S Greenspan SI | 2005 | J Dev Learn Disord2005，，9： | 1 |
| 17 | Effects of Cl-,NO-3 and SO24- ions on anodic dissolution of pure aluminum in alkaline solution显示文摘 | Pyun SI MoonSM Ahn S H etal | 1999 | Corros Sci1999，41，： | 1 |
| 18 | A frequency control method for regulating wireless power to implantable devices显示文摘 | SI Ping HU A P -MALPAS S | 2008 | IEEE Transactions on Biomedical Circuits and Systems2008，2，1： | 1 |
| 19 | Identifying product failure rate based on a conditional Bayesian network classifier显示文摘 | Cai Z Q Sun S D Si S B | 2011 | Expert Systems with Applications2011，38，5： | 1 |
| 20 | A state space-based prognostic model for hidden and age-depend-ent nonlinear degradation process显示文摘 | Feng L Wang H L Si X S | 2013 | IEEE Trans-actions on Automation Science and Engineering2013，10，4： | 1 |
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