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| 1 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 2 | A novel mutation in the thyrotropin(thyroid-stimulating hormone)receptor gene in a case of congenital hypothyroidism显示文摘 | Jeziorowska A Pniewska-Siark B Brzeziańska E | | 0，，： | 1 |
| 3 | Synthesis of linear and cyclic opioid-based peptide analogs containing multiple N-methylated amino acid residues显示文摘 | Adamska A Kolesi fi ska B Kluczyk A | 2015 | J Pept Sci2015，21，11： | 1 |
| 4 | Immunological and biochemical indicators in turkeys fed diets with a different Methionine content显示文摘 | KUBI SKA M TYKAOWSKI B JANKOWSKI J | 2014 | Polish Journal of Veterinary Sciences2014，17，4： | 1 |
| 5 | Impaired glucose metabolism in colorectal cancer显示文摘 | Ehrmann-Jósko A Siemińska J Górnicka B et a1 | 2006 | Scand J Gastroenterol2006，41，9： | 1 |
| 6 | Normal aging and cognition显示文摘 | Ska B Joanette Y | 2006 | Med Sci (Paris)2006，22，： | 1 |
| 7 | Human cord blood-derived cells attain neuronal and glial features in vitro显示文摘 | BUZAN′SKA L MACHAJ E K ZABOCKA B | 2002 | J Cell Sci2002，115，21： | 1 |
| 8 | Neutrophil ge- latinase-associated lipocalin and Cathepsin L as early pre- dictors of kidney dysfunction in children with type 1 diabe- tes显示文摘 | Soltysiak J Skowron'ska B Fichna P | 2014 | Endokrynologia Polska2014，65，6： | 1 |
| 9 | Cardiac rupture in acute myocardial infarction with ST segment elevation, clinical course and prognosis显示文摘 | Janion M Wozakow Ska~kaplon B Sadowski J | 2004 | Kar- diol Pol2004，1，8： | 1 |
| 10 | Preliminary results of investigationon elimination of viruses from apple,pear and raspberry usingthermotherapy and chemotherapy in vitro显示文摘 | Cieslińska M Zawadzka B | | Phy0，，： | 1 |
| 11 | Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI 8226myeloma cells显示文摘 | Zdzisińska B Bojarska-Junak A Dmoszyńska A | 2008 | Arch Immunol Ther Exp2008，56，3： | 1 |
| 12 | Interaction between Leydig and sertoil cells in vitro显示文摘 | Bilińska B | 1989 | Cytobios1989，60，241： | 1 |
| 13 | Atypical imaging features ofadrenal gland lesions in children-report of three cases and review ofliterature显示文摘 | Czerwińska K Roik D Sopy?o B | | 0，，02： | 1 |
| 14 | /? vivo antioxidant activity of procyanidin-rich ex-tracts from grape seed and pine ( Pinus maritima)bark in rats显示文摘 | BUSSEROLLES J GUEUX E BALASIN SKA B etal | 2006 | International Journal for Vitamin andNutrition Research2006，76，1： | 1 |
| 15 | The effect of antidepressant drugs on theHPA axis activity glucocorticoid receptor level andFKBP51 concentration in prenatally stressed rats 显示文摘 | Szyman ska M Budziszewska B Jaworska-FeilL | 2009 | Psychoneuroendocrinology2009，34，6： | 1 |
| 16 | Expression of metallothioein in renal tubules of rats exposed to acute and endurance exercise显示文摘 | Podhorska Okoów M Dziegiel P Dolińska Krajewska B | 2006 | Folia Histochemicaet Cytobiological2006，44，3： | 1 |
| 17 | Impaired glucose metabolism in colorectal cancer显示文摘 | Ehrmann-Jósko A Siemińska J Górnicka B | 2006 | Scand J Gastroenterol2006，41，9： | 1 |
| 18 | Serum YKL-40 levels in patients with ovarian cancer and women with BRCA1 gene mutation-comparison to CA 125 antigen显示文摘 | Chudecka-Glaz A Górski B Zielińska D | 2009 | Eur J Gynaecol Oncol2009，30，6： | 1 |
| 19 | Effect of peak concentrations on the neurotoxicity of styrene in volunteers显示文摘 | B Ska A Vyskocil R Tardif | 2003 | Human & Experimental Toxicology2003，22，： | 1 |
| 20 | Self-efficacy and anxiety and depression in patients with colostomy显示文摘 | Gapińska B Tartas M Walkiewicz M | 2008 | Wspolczesna Onkologia2008，12，2： | 1 |
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