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| 1 | Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds. | Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes | 2017 | World Journal of Gastroenterology2017，23，2： | 6 |
| 2 | Nat Genet:单基因突变导致过敏性皮炎的发生显示文摘最近,研究者们鉴定出了一类导致神经性皮炎发生的关键基因突变:CARDll。来自美国NIH过敏与传染病研究所的研究者们通过对四个没有血缘关系的患病家庭进行分析,发现了这一导致疾病产生的基因、 | Chi A Ma, Yuan Zhang, Michael A Weinreich, Jonathan J Lyons, Celeste G Nelson, Thomas DiMaggio, Kelly D Stone, Joshua D Milner Jeffrey R Stinson, Elisa Ruffo, Batsukh Dorjbal, Swadhinya Arjunaraja, Kelsey Voss, Andrew L Snow Jordan K Abbott, Pia J Hauk, Paul R Reynolds, Erwin W Gelfand Elisa Ruffo Salomé Glauzy, Natsuko Yamakawa, Eric Meffre Jennifer Stoddard, Julie Niemela, Sergio D Rosenzweig Yu Zhang, Helen F Matthews Joshua J McElwee Nina Jones Alejandro Palma, Matías Oleastro, Emma Prieto, Andrea R Bernasconi, Geronimo Dubra, Silvia Danielian, Jonathan Zaiat, Marcelo A Marti Brian Kim Megan A Cooper Neil Romberg | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 3 | A video database of moving faces and people显示文摘 | O'TOOLE A J HARMS J SNOW S L | 2005 | IEEE Transactions on Pattern Analysis and Machine Intelligence2005，27，5： | 1 |
| 4 | Expression Kinetics of interferon and interferon-induced genes in Atlan- tic salmon ( Salmo salar ) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia vi- rus 显示文摘 | Mc Beath A J Snow M Secombes C J | 2007 | Fish Shellfish Immunol2007，22，3： | 1 |
| 5 | Subeulaneous panniculitic T-cell lymphoma phoma and cytophagic histocytic panniculitis 显示文摘 | Yung A Snow J Jarrell P | 2001 | Austraia J Dermatol Matol2001，42，3： | 1 |
| 6 | A family of thermostable fungal cellulases created by structure-guided recombination 显示文摘 | Heinzelman P Snow CD Wu I Nguyen C Villalobos A Govindarajan S Minshull J Arnold FH | 2009 | PNAS2009，106，： | 1 |
| 7 | Distant metastases of adenoid cystic carcinoma of the salivary glands and the value of diagnostic exami- nations during follow-up显示文摘 | VAN DER WAL J E BECKING A G SNOW G B | 2002 | Head Neck2002，24，： | 1 |
| 8 | Expression kinetics of interferon and interferon-induced genes in Atlantic salmon (Salmo salar) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia virus 显示文摘 | McBeath A J A Snow M Secombes C J | 2007 | Fish & Shellfish Immunology2007，22，3： | 1 |
| 9 | Degos' syndrome:malignant atrophic papulosis显示文摘 | Snow J L Muller S A | 1995 | Semin Dermatol1995，14，： | 1 |
| 10 | Oligodimethyl- siloxane Linked Cyanate Ester Resins显示文摘 | Maya E M Snow A W Buckley L J | 2002 | Macromolecules2002，35，2： | 1 |
| 11 | Imaging of Budd Chiari syndrome显示文摘 | Buckley O Obrien J Snow A | 2007 | Eur Radiol2007，17，11： | 1 |
| 12 | Imaging of Budd-Chiari syndrome 显示文摘 | Buckley O O'Brien J Snow A | 2007 | Eur Radiol2007，17，8： | 1 |
| 13 | An overview of APSIM, a model designed for farming systems simulation 显示文摘 | Keating B A Carberry P S Hammer G L Probert M E Robertson M J Holzworth D Huth N I Hargreaves J N G Meinke H Hochman Z McLean G Verburg K Snow V Dimes J P Silburn M Wang E Brown S Bristow K L Asseng S Chapman S McCown R L Freebairn D M Smith C J | 2003 | European Journal of Agronomy2003，18，314： | 1 |
| 14 | Fecundity of transgenic wild-crop hybrids of Cucurbita pepo(Cucurbitaceae): implications for crop-to-wild gene flow 显示文摘 | Spencer L J Snow A A | 2001 | Heredity2001，86，： | 1 |
| 15 | Syntheses and characterization of heteroatom-bridged metal-free phthalocyanine network polymers and model compounds显示文摘 | Snow A W Griffith J R | 1984 | Macromolecules1984，17，8： | 1 |
| 16 | Botulinum toxin A in the treatment of patients with Frey syndrome 显示文摘 | Beerens A J Snow GB | 2002 | BrJ Surg2002，89，1： | 1 |
| 17 | Dysnatremias and Survival in Adult Burn Patients: A Retrospective Analysis显示文摘 | Stewart Ian J Morrow Benjamin D Tilley Molly A Snow Brian D Gisler Christopher Kramer Keith W Aden James K Renz Evan M Chung Kevin K | 2013 | American Journal of Nephrology2013，，1： | 1 |
| 18 | Organizational strategy, structure, and process 显示文摘 | MILES R E SNOW C C MEYER A D COLEMAN J R | 1978 | Academy of Management Review1978，，3： | 1 |
| 19 | Drosophila neuroglian:a mem-ber of the immunoglobulin superfamily with extensive homology tothe vertebrate neural adhesion molecule Ll显示文摘 | Bieber A J Snow P M Hortsch M | 1989 | Cell1989，59，3： | 1 |
| 20 | The coexistence of neuropeptides in feline sensory neurons 显示文摘 | CAMERSON A A LEAH J D SNOW P J | 1988 | Neuroscience1988，，27： | 1 |
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