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| 1 | 培养条件及表面活性剂的添加对纳豆芽孢杆菌生物膜形成的影响显示文摘采用改良的微孔板法培养纳豆芽孢杆菌(Bacillus subtilis natto),通过结晶紫染色定量分析产生的生物膜。结果表明:培养条件为pH 7、温度37℃、培养72 h且在5 g/100 mL葡萄糖-5 g/100 mL NaCl条件下成膜能力最佳。柠檬酸二铵和聚乙二醇-200(polyethylene glycol-200,PEG-200)随着质量浓度的增加对生物膜的形成量呈现先下降后上升的趋势,十二烷基磺酸钠(sodium dodecyl sulfate,SDS)抑制生物膜的形成,十六烷基三甲基溴化铵(cetyl trimethyl ammonium bromide,CTAB)促进生物膜的形成。所以低温抑制生物膜的形成,一定浓度的NaCl和葡萄糖促进生物膜形成,不同的表面活性剂对其生物膜的影响机制不同,为纳豆芽孢杆菌生物膜的研究提供理论基础。 | 冯静静 杨自名 吴静 李伟 Sokhna Mbacke Gningue 赵礼军 周文豪 薛正莲 王洲 刘艳 | 2020 | 食品与发酵工业2020，46，3： | 7 |
| 2 | 不同培养条件对枯草芽胞杆菌BS168-ΔsinR生物膜形成及维生素K2产量的影响显示文摘枯草芽胞杆菌(Bacillus subtilis)是合成维生素K2的微生物之一,维生素K2的合成量又与细菌生物膜有重要关系。其中sinR是生物膜形成环节中的关键调节基因,我们用同源重组的方法构建枯草芽胞杆菌sinR缺失突变体(ΔsinR)菌株,再以BS168-ΔsinR菌株为出发菌株,采用微孔板法-结晶紫染色测定生物膜形成量,核酸定量仪测定胞外蛋白,苯酚硫酸法测定胞外多糖,HPLC测定维生素K2的生物合成量。比较分析了不同温度、pH、以及不同浓度的金属离子(Mn^(2+)、 Fe^(2+)、Ca^(2+))对枯草芽胞杆菌BS168及BS168-ΔsinR生物膜形成能力以及维生素K2合成量的影响。结果表明,重组菌BS168-ΔsinR生物膜形成能力和维生素K2合成能力均优于原始菌BS168,且重组菌在生物膜形成、维生素K2合成过程中对温度、酸碱性的适应能力也强于原始菌。一定浓度的金属离子可以促进生物膜的形成及维生素K2的合成。2株菌生物膜形成的最佳条件为温度37~40℃、pH 8.0、Mn^(2+)浓度0.01 mmol/L、 Fe^(2+)浓度1μmol/L、Ca^(2+)浓度0.01 mmol/L,维生素K2合成的最佳条件是温度40℃、pH 7.0~8.0、Mn^(2+)浓度0.1 mmol/L、 Fe^(2+)浓度50μmol/L、Ca^(2+)浓度1 mmol/L。该研究为深入探究sinR基因参与枯草芽胞杆菌生物膜形成的机制及提高维生素K2合成产量提供实验基础和理论支撑。 | 吴静 李伟 冯静静 周梦洁 胡汶松 汪剑 SOKHNA MBACKE Gningue 吴佳雯 赵礼军 徐文瀚 薛正莲 王洲 刘艳 | 2021 | 食品与发酵工业2021，47，14： | 4 |
| 3 | The paradigm of protein acetylation in Parkinson's disease显示文摘Acetylation is a post-translational modification that is regulated by two antagonistic enzymes,histone acetyltransferases(HATs)and histone deacetylases(HDACs).HATs transfer the acetyl group from acetyl-CoA to lysine residues of proteins while HDACs remove it(Yakhine-Diop et al.,2018b).The impairment of HAT or HDAC activity elicits changes in the protein acetylation status which disturb several cellular processes,among others,gene expression,autophagy etc.,leading finally to cell death.Both enzymes are associated with Parkinson’s disease(PD)pathogenesis.In dopaminergic cells,neurotoxins provoke apoptotic cell death by increasing histone acetylation levels.While paraquat(Song et al.,2011)and rotenone(Feng et al.,2015)reduce HDAC activity,dieldrin(Song et al.,2010)enhances HAT activity.However in vivo,paraquat-induced upregulation ofα-synuclein triggers histone hypoacetylation. | Sokhna M.S.Yakhine-Diop Guadalupe Martínez-Chacón Elisabet Uribe-Carretero Mireia Niso-Santano Rosa A.González-Polo José M.Fuentes | 2019 | Neural Regeneration Research2019，14，6： | 2 |
| 4 | Worms can worsen malaria: towards a new means to roll back malaria 显示文摘 | Druilhe P Tall A Sokhna C | 2005 | Trends Parasitol2005，21，8： | 1 |
| 5 | Host factors affecting the delay of reappearance of Plasmodium falciparurn after radical treatment among a semi-immune population exposed to intense perennial transmission显示文摘 | Keyier C Dieye A | 2000 | Am J Trop Med Hyg2000，62，2： | 1 |
| 6 | G2019S LRRK2 mutant fibroblasts from Parkinson's disease patients show increased sensitivity to neurotoxin l-methyl-4- phenylpyridinium dependent of autophagy显示文摘 | Sokhna M S Yakhine-Diop Jose M Bravo-San Pedro Ruben Gomez-Sanchez R | 2014 | Toxicology2014，324，3： | 1 |
| 7 | Prevalence of hepatitis B markers in Senegalese HIV‐1‐infected patients显示文摘 | Gora L? Amina Sow‐Sall Halimatou Diop‐Ndiaye Nokoa Chadia Ines Danty Mandiouba Moussa Thiam Fatou Diop Ousseynou Ndiaye Sokhna Bousso Gueye Sidy Mouhamed Seck Abou Abdallah Malick Dioura Moustapha Mbow A?ssatou Gaye‐Diallo Souleymane Mboup Coumba Touré‐Ka | 2016 | J. Med. Virol2016，，： | 1 |
| 8 | Prevalence of diarrhoea and risk factors among children under five years old in Mbour,Senegal:a cross-sectional study显示文摘Background:Diarrhoeal diseases remain an important cause of mortality and morbidity among children,particularly in low-and middle-income countries.In Senegal,diarrhoea is responsible for 15%of all deaths in children under the age of five and is the third leading cause of childhood deaths.For targeted planning and implementation of prevention strategies,a context-specific understanding of the determinants of diarrhoeal diseases is needed.The aim of this study was to identify risk factors of diarrhoeal diseases in children under the age of five in Mbour,Senegal.Methods:Between February and March 2014,a cross-sectional survey was conducted in four zones of Mbour to estimate the burden of diarrhoeal diseases(i.e.diarrhoea episodes in the 2 weeks preceding the survey)and associated risk factors.The zones covered urban central,peri-central,north peripheral and south peripheral areas.Overall,596 households were surveyed by a questionnaire,yielding information on sociodemographic,environmental and hygiene behavioural factors.Univariable and multivariable logistic regression analyses were used to identify risk factors associated with the occurrence of diarrhoea.Results:The reported prevalence of diarrhoea among children under the age of five during the 2 weeks preceding the survey was 26%.Without adjustment,the highest diarrhoea prevalence rates were observed in the peri-central(44.8%)and urban central zones(36.3%).Multivariable regression revealed significant associations between diarrhoeal diseases and unemployment of mothers(adjusted odds ratio[aOR]=1.62,95%confidence interval[CI]:1.18-2.23),use of open bags for storing household waste(aOR=1.75,95%CI:1.00-3.02),evacuation of household waste in public streets(aOR=2.07,95%CI:1.20-3.55),no treatment of stored drinking water(aOR=1.69,95%CI:1.11-2.56)and use of shared toilets(aOR=1.69,95%CI:1.11-2.56).Conclusion:We found a high prevalence of diarrhoea in children under the age of five in Mbour,with the highest prevalence occurring in the central and peri-central areas.These findings underscore the need for public health interventions to alleviate the burden of diarrhoea among vulnerable groups.Promotion of solid waste disposal and reduction of wastewater exposure should be implemented without delay. | Sokhna Thiam Aminata N.Diene Samuel Fuhrimann Mirko S.Winkler Ibrahima Sy Jacques A.Ndione Christian Schindler Penelope Vounatsou Jurg Utzinger Ousmane Faye Gueladio Cisse | 2017 | Infectious Diseases of Poverty2017，6，1： | 1 |
| 9 | Seasonal intermittent preventive treatment with artesunate and sulfadoxine-pyrimethamine for prevention of malaria in Senegalese children: a randomised, placebo-controlled, double-blind trial显示文摘 | Badara Cissé Cheikh Sokhna Denis Boulanger Jacqueline Milet El Hadj Ba Keshena Richardson Rachel Hallett Colin Sutherland Kirsten Simondon Fran?ois Simondon Neal Alexander Oumar Gaye Geoffrey Targett Jo Lines Brian Greenwood Jean-Fran?ois Trape | 2006 | The Lancet2006，，9511： | 1 |
| 10 | The dual role of necrostatin-1 in Parkinson’s disease models显示文摘Neuronal cell death is the main hallmark of Parkinson’s disease(PD).It is an irreversible process promoted by neurotoxins and/or genetic mutations.Different types of cell death have been associated with PD.The mechanisms by which neurons decide to specific type of cell death remain elusive.However,it is well known that cell death can be either programmed or not.Apoptosis is a programmed cell death that involves the release of cytochrome c from damaged mitochondria to cytosol and the activation of caspases leading to nuclear condensation.Necrosis is a caspase-independent cell death characterized by a gain in cell volume,rupture of plasma membrane and leak of cell contents,inflammation,and affects neighbouring cells.It was classified as a nonprogrammed cell death,but there are types of necrotic death triggered by a protein activation cascade,including necroptosis. | Eva Alegre-Cortes Guadalupe Martinez-Chacon Jose M.Fuentes Sokhna M.S.Yakhine-Diop | 2021 | Neural Regeneration Research2021，16，10： | 0 |
| 11 | 通过绿色长城倡议在萨赫勒地区防治荒漠化并改善当地生计:以塞内加尔为例(英文)显示文摘绿色长城倡议的总目标是采取土地可持续管理的可靠实践以及加强和保护自然资源和生产转化系统来防止荒漠扩张,同时通过多用途活动平台来保证当地社区民众的社会经济发展。本研究描述的相关活动旨在实现如下目标:(1)提供财富;(2)加强获取基本社会服务;(3)实现向绿色经济的转变,为建设乡村生产中心创造良好条件;(4)实现可持续发展,以减少贫困和提高粮食安全;(5)增强当地人口的气候变化适应能力。本研究利用的信息来自塞内加尔实施绿色长城倡议相关的众多出版物和文档,包括科学论文、学位论文、会议纪要和报告等。 | Salif Diop Aliou Guisse Claude Sene Birane Cisse Ndeye Rokhaya Diop Sokhna Die Ka Amady Gnagna Cisse Saly Sambou Ousmane Ndiaye Adandé Belarmain Fandohan 付超 王国勤 王永东 | 2018 | Journal of Resources and Ecology2018，9，3： | 0 |
| 12 | 枯草芽胞杆菌异戊二烯酶ComQ的生物信息学分析及对生物膜形态的影响显示文摘【目的】枯草芽胞杆菌ComQ是一种类异戊二烯生物合成酶。利用生物信息学预测分析了ComQ的生物学特性,对comQ基因进行过表达和敲除,构建突变菌,孔板发酵培养验证生物膜形态变化。【方法】运用NCBI(National Center for Biotechnology Information)网站里的Protein数据库获取ComQ蛋白氨基酸序列,通过在线生物信息学软件预测分析其生物学特征,包括其理化性质、信号肽、结构域、空间结构等。构建枯草芽胞杆菌BS168的comQ过表达及敲除菌株,利用孔板发酵培养验证生物膜生长性状。【结果】ComQ蛋白由299个氨基酸组成,理论相对分子质量约34204.08 Da,无信号肽,无跨膜区,为稳定胞内蛋白。通过菌落PCR,验证枯草芽胞杆菌BS168突变菌构建成功。利用孔板发酵培养枯草芽胞杆菌BS168及突变菌株,验证ComQ蛋白对枯草芽胞杆菌生物膜的形态形成存在影响。两种突变菌株生物膜形态均与原始菌株存在差异。【结论】通过对ComQ蛋白的基本性质、关键氨基酸位点及蛋白结构预测分析,构建突变菌株,验证生物膜形态变化,为后续探究ComQ对枯草芽胞杆菌的生长代谢变化奠定了基础。 | 李伟 吴静 冯静静 汪剑 周梦洁 胡汶松 Sokhna mbacke Gningue 薛正莲 赵世光 钱森和 王洲 刘艳 | 2022 | 微生物学报2022，62，2： | 0 |
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