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| 1 | Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy显示文摘AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic lesions.METHODS:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon tumors.eUBM was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM imaging.The ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM images.During image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall anatomy.During the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon wall.Once the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and eosin.Colon images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion progression.RESULTS:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and thus,only three were subjected to the third eUBM/colonoscopy imaging acquisition(the third time-point).eUBM was able to detect the four layers in healthy segments of colon:the mucosa(the first hyperechoic layer moving away from the mini-probe axis),followed by the muscularis mucosae(hypoechoic),the submucosa(the second hyperechoic layer)and the muscularis externa(the second hypoechoic layer).Hypoechoic regions between the mucosa and the muscularis externa layers represented lymphoid infiltrates,as confirmed by the corresponding histological images.Pedunculated tumors were represented by hyperechoic masses in the mucosa layer.Among the lesions that decreased in size between the first and third time-points,one of the lesions changed from a mucosal hyperplasia with ulceration at the top to a mucosal hyperplasia with lymphoid infiltrate and,finally,to small signs of mucosal hyperplasia and lymphoid infiltrate.In this case,while lesion regression and modification were observable in the eUBM images,colonoscopy was only able to detect the lesion at the first and second time-points,without the capacity to demonstrate the presence of lymphoid infiltrate.Regarding the lesions that increased in size,one of them started as a small elevation in the mucosa layer and progressed to a pedunculated tumor.In this case,while eUBM imaging revealed the lesion at the first time-point,colonoscopy was only able to detect it at the second time-point.All colonic lesions(tumors,lymphoid infiltrate and mucosal thickening)were identified by eUBM,while colonoscopy identified just76%of them.Colonoscopy identified all of the colonic tumors but failed to diagnose lymphoid infiltrates and increased mucosal thickness and failed to differentiate lymphoid infiltrates from small adenomas.During the observation period,most of the lesions(approximately67%)increased in size,approximately 14%remained unchanged,and 19%regressed.CONCLUSION:Combining eUBM with colonoscopy improves the diagnosis and the follow-up of mouse colonic lesions,adding transmural assessment of the bowel wall. | Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado | 2013 | World Journal of Gastroenterology2013，19，44： | 1 |
| 2 | Hydroxyapa- tite-collagen-hyaluronic acid composite 显示文摘 | Bakos D Soldan M Hermandez-Fuentes I | 1999 | Biomateri- als1999，20，2： | 1 |
| 3 | Features of In Vitro Ultrasound Biomicroscopic Imaging and Colonoscopy for Detection of Colon Tumor in Mice显示文摘 | Kelly Z. Alves Helena L. Borges Rossana C. Soletti Anália L.P. Viana Lorena I. Petrella M?nica Soldan Vera L. Chagas Alberto Schanaider Jo?o C. Machado | 2011 | Ultrasound in Medicine & Biology2011，，12： | 1 |
| 4 | Two-dimensional electrophoresis :a powerful method to elucidate cellular responses to toxic compounds显示文摘 | Moiler A Soldan M Volker U | 2001 | Toxicology2001，160，13： | 1 |
| 5 | Cognitive changes preceding clinical symptom onset of mild cognitive impairment and relationship to ApoE genotype 显示文摘 | Albert M Soldan A Gottesman R | 2014 | Curr Alzheimer Res2014，11，8： | 1 |
| 6 | Hydroxyapa-tite-collagen-hyaluronic acid composite 显示文摘 | Bakos D Soldan M Hernandez-Fuentes I | 1999 | Biomateri-als1999，20，： | 1 |
| 7 | Hydroxyapatite-collagen-hyaluronic acid composite显示文摘 | Bakos D Soldan M Hernandez-Fuentes I | 1999 | Biomaterials1999，20，： | 1 |
| 8 | Hydroxyapatiie-collagenhyaluronic acid composite显示文摘 | Bakos D Soldan M Hemandez-Fuentes i | 1999 | Biomaterials1999，20，： | 1 |
| 9 | Two-dimensional gel electrophoresis: a powerful method to elucidate cellular responses to toxic compounds显示文摘 | Mller A Soldan M Vlker U Maser E | 2000 | Toxicology2000，160，13： | 1 |
| 10 | Hydroxyapatite- collagen-hyaluronic acid composite 显示文摘 | Bakos D Soldan M Hernandez Fuentes I | 1999 | Biomaterial1999，20，2： | 1 |
| 11 | Hydroxyapatite-collagen-hyaluronic acid composite显示文摘 | Bakos D Soldan M Hernandez-Fentes I | 1999 | Biomaterials1999，20，： | 1 |
| 12 | Crosse virus nonstructural protein NSs counteracts the effects of short interfering RNA 显示文摘 | Soldan S S Plassmeyer M L Matukonis M K | 2005 | Journal of Virology2005，79，： | 1 |
| 13 | Viscometric Study of the Sodium Hyaluronate-Sodium Chloride-alkyl-(n)-ammonium Surfactant System显示文摘 | Pisárcik M Soldan M Bakos D | 1999 | Colloids Surf A1999，150，： | 1 |
| 14 | Hydroxyapatite-collagen-hyaluronic acid composite显示文摘 | Bakos D Soldan M Hernandez-Fuentes I | 1999 | Biomaterials1999，20，2： | 1 |
| 15 | Two-dimensional gel electrophoresis: a powerful method to elucidate cellular responses to toxic eompond 显示文摘 | Moller A Soldan M Volker U | 2001 | Toxicology2001，160，13： | 1 |
| 16 | Baia mare accident-Brief ecotoxicological report of czech experts显示文摘 | SOLDAN P PAVONIC M BOUCEK J | 2001 | Ecotoxicology and Environmental Safety2001，49，： | 1 |
| 17 | Hydroxyapatite collagen hyaluronic acid composite 显示文摘 | Bakos D Soldan M Hemadez-Fuentes I | 1999 | Biomaterials1999，20，2： | 1 |
| 18 | Hydroxyapatite collagen hyaluronic acid compoasite显示文摘 | Bakos D Soldan M Fuentes IH | 1999 | Biomaterials1999，20，： | 1 |
| 19 | Two-dimensional gel electrophoresis:a powerful method to elucidate cellular responses to toxic compounds显示文摘 | Moller A Soldan M Volker U | 2001 | Toxicology2001，160，13： | 1 |
| 20 | Two-dimentional gel electrophoresis: a powerful method to elucidate cellular responses to toxic compounds显示文摘 | Moller A Soldan M Volker U | 2001 | Toxicology2001，160，： | 1 |
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