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| 1 | Control of postharvest green mold of citrus fruit with yeasts, medicinal plants, and their combination显示文摘 | Henik Sukorini Somsiri Sangchote Netnapis Khewkhom | 2013 | Postharvest Biology and Technology2013，，： | 2 |
| 2 | Initial rotor po- sition estimation for sensorless brushless DC drivers 显示文摘 | Champa P Somsiri P Wipasuramonton P | 2009 | IEEE Transactions on Industry Applications2009，45，4： | 1 |
| 3 | Initial Brushless DC drives显示文摘 | Champa P Somsiri P Wipasuramonton rotor position estimation for sensorless P | 2009 | IEEE Transactions on Industry Applications2009，45，4： | 1 |
| 4 | Targeted transfollicular delivery of artocarpin extract from Artocarpus incisus by means of microparticles显示文摘 | Pitaksuteepong T Somsiri A Waranuch N | 2007 | Ear J Pharm Biopharm2007，67，3： | 1 |
| 5 | Adaptive Inte- gral Sliding-mode Position Control of a Coupled-phase Linear Variable Reluctance Motor for High- precision Applications 显示文摘 | Kachapomkul S Jitkreeyarn P Somsiri P | 2012 | Industry Applications2012，48，4： | 1 |
| 6 | Initial rotor position estimation for sensorless brushless DC drives显示文摘 | Parasit Champe Pakasit Somsiri Pongpit Wipasuramonton | 2009 | IEEE Transactions on Industry Applications2009，45，4： | 1 |
| 7 | Initial Rotor Position Estimation for Sensorless Brushless DC Drives显示文摘 | Champa P Somsiri P Wipasuramonton P | 2009 | IEEE Transactions on Industry Applications2009，45，4： | 1 |
| 8 | Initial rotor position estimation for sensorless brushless DC drives显示文摘 | Champa P Somsiri P Wipasuramonton P | | 0，，04： | 1 |
| 9 | γ-aminobutyric acid B2 receptor:A potential therapeutic target for cholangiocarcinoma in patients with diabetes mellitus显示文摘BACKGROUND The association between diabetes mellitus(DM)and the increased risk and progression of cholangiocarcinoma(CCA)has been reported with unclear underlying mechanisms.Previous studies showed thatγ-aminobutyric acid(GABA)B2 receptor(GABBR2)was upregulated in CCA cells cultured in high glucose(HG)conditions.Roles of GABA receptors in CCA progression have also been studied,but their association with DM and hyperglycemia in CCA remains unclarified.AIM To investigate the effects of hyperglycemia on GABBR2 expression and the potential use of GABBR2 as a CCA therapeutic target.METHODS CCA cells,KKU-055 and KKU-213A,were cultured in Dulbecco Modified Eagle’s Medium supplemented with 5.6 mmol/L(normal glucose,NG)or 25 mmol/L(HG)glucose and assigned as NG and HG cells,respectively.GABBR2 expression in NG and HG cells was investigated using real-time quantitative polymerase chain reaction and western blot.Expression and localization of GABBR2 in CCA cells were determined using immunocytofluorescence.GABBR2 expression in tumor tissues from CCA patients with and without DM was studied using immunohistochemistry,and the correlations of GABBR2 with the clinicopathological characteristics of patients were analyzed using univariate analysis.Effects of baclofen,a GABA-B receptor agonist,on CCA cell proliferation and clonogenicity were tested using the MTT and clonogenic assays.Phospho-kinases arrays were used to screen the affected signaling pathways after baclofen treatment,and the candidate signaling molecules were validated using the public transcriptomic data and western blot.RESULTS GABBR2 expression in CCA cells was induced by HG in a dose-and time-dependent manner.CCA tissues from patients with DM and hyperglycemia also showed a significantly higher GABBR2 expression compared with tumor tissues from those with euglycemia(P<0.01).High GABBR2 expression was significantly associated with a poorer non-papillary histological subtype but with smaller sizes of CCA tumors(P<0.05).HG cells of both tested CCA cell lines were more sensitive to baclofen treatment.Baclofen significantly suppressed the proliferation and clonogenicity of CCA cells in both NG and HG conditions(P<0.05).Phospho-kinase arrays suggested glycogen synthase kinase 3(GSK3),β-catenin,and the signal transducer and activator of transcription 3(STAT3)as candidate signaling molecules under the regulation of GABBR2,which were verified in NG and HG cells of the individual CCA cell lines.Cyclin D1 and c-Myc,the common downstream targets of GSK3/β-catenin and STAT3 involving cell proliferation,were accordingly downregulated after baclofen treatment.CONCLUSION GABBR2 is upregulated by HG and holds a promising role as a therapeutic target for CCA regardless of the glucose condition. | Charupong Saengboonmee Supannika Sorin Sakkarn Sangkhamanon Surang Chomphoo Somsiri Indramanee Wunchana Seubwai Kanyarat Thithuan Ching-Feng Chiu Seiji Okada Marie-Claude Gingras Sopit Wongkham | 2023 | World Journal of Gastroenterology2023，29，28： | 0 |
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