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| 1 | 基于蛋白质组学的黄芪多糖对TLR4激活的肺癌细胞来源外泌体的作用研究显示文摘黄芪多糖(Astragalus polysaccharides, APS)是黄芪的主要有效成分之一,可通过调节肿瘤炎症微环境发挥抗肿瘤作用。外泌体是粒径50~200 nm的小细胞外囊泡,携带有来自亲本细胞的核酸和蛋白质成分。当与受体细胞结合,外泌体可以发挥细胞间通讯和调节免疫反应等作用。该研究采用超速离心法,对Toll样受体4(Toll-like receptor 4, TLR4)激活的肺癌细胞H460炎症状态组和APS缓解组H460细胞释放的外泌体进行了提取,并利用蛋白免疫印迹、透射电镜和纳米粒径跟踪分析对外泌体进行表征。进一步通过非标记蛋白质组学方法对3组H460细胞来源的外泌体蛋白进行分析,在3组中分别鉴定到897、800和911个蛋白质,其中88%的蛋白质属于ExoCarta外泌体蛋白质数据库;差异统计分析显示有111个蛋白质表达量在LPS组和APS组均有显著变化(P<0.05)。针对差异蛋白质进行生物学信息分析,与差异蛋白质相关的分子功能、生物过程和信号通路主要涉及病毒过程、蛋白质结合、蛋白酶体和上皮细胞细菌入侵等过程。关键差异蛋白包括纤溶酶原激活物抑制剂-1、层黏连蛋白α5、层黏连蛋白α1和CD44等,说明不同状态下肿瘤细胞会发生系统性变化,并体现在炎症微环境的外泌体中;分析结果也提示APS可能通过TLR4/MyD88/NF-κB信号通路或调节细胞外基质影响炎症微环境。该研究有助于了解炎症状态下肿瘤发展机制,并在外泌体层面上探索APS的抗炎作用。 | 胡康蝶 杨凯歌 SOUMIA Cheddah 吴明媛 闫超 李欣燕 王彦 | 2022 | 中国中药杂志2022，47，21： | 8 |
| 2 | 加压毛细管电色谱法高效分离分析葛根多糖中的单糖显示文摘葛根多糖具有抗氧化、抗肿瘤等众多生物活性,对葛根多糖进行单糖组成分析对其活性研究具有重要意义。该研究利用响应面分析法考察了超声辅助提取法中液料比、超声温度、超声时间和超声功率对葛根多糖提取率的交互影响,并拟合数据得到多元二次回归方程。同时建立了柱前衍生加压毛细管电色谱检测糖类的方法,对分离8种中性单糖的色谱条件进行了探索与优化,并将此方法应用于两种葛根实际样品的单糖组成测定。响应面分析结果表明,4个试验因素中,超声温度对两种葛根多糖提取率的影响程度最大,其次为液料比,超声时间和超声功率影响程度较小。结合软件预测分析得到的最佳条件及设备实际情况,确定葛根多糖的最佳提取工艺条件为:超声温度90℃,粉葛多糖液料比20 mL/g,柴葛多糖液料比40 mL/g,超声时间30 min,超声功率180 W。优化后的色谱分离条件为:采用Halo-2.7μm核壳型C18填料毛细管色谱柱,以乙腈-50 mmol/L pH 4.1的醋酸铵水溶液(18∶82,v/v)为流动相,在250 nm波长下检测,施加电压-20 kV。在此条件下可以实现24 min内对葡萄糖等8种中性单糖衍生物的快速分离,相比传统液相色谱方法大大提升了分离检测速度和分离柱效。方法学考察表明此方法具有较好的线性关系和良好的重复性。对实际样品分离鉴定表明,粉葛多糖主要由葡萄糖、甘露糖、鼠李糖和岩藻糖组成,4种单糖物质的量之比为1.00∶0.16∶0.14∶0.07;柴葛多糖主要由葡萄糖和甘露糖组成,2种单糖物质的量之比为1.00∶0.70。该研究为单糖化合物快速高效分离检测提供了新方法,并为葛根多糖单糖组成分析提供了参考。 | 许歆瑶 Soumia CHEDDAH 王彦 阎超 | 2020 | 色谱2020，38，11： | 3 |
| 3 | Sequential extraction of heavy metals during composting of sewage sludge显示文摘 | Soumia Amir Mohamed Hafidi Georges Merlina Jean-Claude Revel | 2004 | Chemosphere2004，，6： | 2 |
| 4 | Vehicle dispatching with timedependent travel times显示文摘 | Soumia I Michel G Jean Y | 2003 | European Journal of Operational Research2003，144，2： | 1 |
| 5 | Decreased heart rate variability may predict the progression of carotid atherosclerosis in type 2 diabetes显示文摘 | Dr. Anders Gotts?ter ?sa Rydén Ahlgren Soumia Taimour G?ran Sundkvist | 2006 | Clinical Autonomic Research2006，，3： | 1 |
| 6 | Chemical and spectroscopic analysis of organic matter transformation during composting of sewage sludge and green plant waste 显示文摘 | Abdelmajid J Soumia A Mohamed E G | 2005 | Int Biodeter Biodeg2005，56，2： | 1 |
| 7 | Structural study of humic acids during composting of activatedsludge-green waste:Elemental analysis,FTIR and 13C NMR显示文摘 | SOUMIA AMIRA ABDELMAJID JOURAIPHYB ABDELILAH MEDDIC-HB | 2010 | Journalof Hazardous Materials2010，177，： | 1 |
| 8 | Sequential extraction of heavy metals during composting of sewage sludge显示文摘 | Soumia A Mohamed H Georges M | 2005 | Chemosphere2005，59，6： | 1 |
| 9 | Chemical and spectroscopic analysis of organic matter transformation during composting of sewage sludge and green plant waste显示文摘 | Abdelmajid Jouraiphy Soumia Amir Mohamed El Gharous Jean-Claude Revel Mohamed Hafidi | 2005 | International Biodeterioration & Biodegradation2005，，2： | 1 |
| 10 | Molecular behaviour of humic acid-like substances during co-composting of olive mill waste and the organic part of municipal solid waste显示文摘 | Farid Barje Loubna El Fels Houda El Hajjouji Soumia Amir Peter Winterton Mohamed Hafidi | 2012 | International Biodeterioration & Biodegradation2012，，： | 1 |
| 11 | Chemical and spectroscopic analysis of organic matter transformation during composting of sewage sludge and green plant waste显示文摘 | Abdelmajid Jouraiphy Soumia Amir Mohamed El Gharous Jean-Claude Revel Mohamed Hafidi | 2005 | International Biodeterioration & Biodegradation2005，，2： | 1 |
| 12 | Hybrid cascaded H-bridge multilevel inverter induction-motor-drive direct torque control for automotive applications显示文摘 | Farid K Soumia M L Khoudir Mi | 2010 | IEEE Transactions on Industrial Electronics2010，57，3： | 1 |
| 13 | Vehicle dispatching with time-dependent travel times显示文摘 | Soumia I Michel G Potvin J Y | 2003 | European Journal of Operational Research2003，144，2： | 1 |
| 14 | Classification et nomenclature de divers dinoflagellés marins(Dinophyceae)显示文摘 | Soumia A | | 0，，： | 1 |
| 15 | Sequential extraetion of heavymetals during eomposting of sew- age sludge显示文摘 | Soumia A Mohamed H Georges M | 2005 | Chemosphere2005，59，： | 1 |
| 16 | Low cost synthesis of LiFePO 4 /C cathode materials with Fe 2 O 3显示文摘 | Lifeng Cheng Guoxian Liang Soumia El Khakani Dean D. MacNeil | 2013 | Journal of Power Sources2013，，： | 1 |
| 17 | Using NWP to Assess the Influence of the Arctic Atmosphere on Midlatitude Weather and Climate显示文摘The influence of the Arctic atmosphere on Northern Hemisphere midlatitude tropospheric weather and climate is explored by comparing the skill of two sets of 14-day weather forecast experiments using the ECMWF model with and without relaxation of the Arctic atmosphere towards ERA-Interim reanalysis data during the integration. Two pathways are identified along which the Arctic influences midlatitude weather: a pronounced one over Asia and Eastern Europe, and a secondary one over North America. In general, linkages are found to be strongest(weakest) during boreal winter(summer) when the amplitude of stationary planetary waves over the Northern Hemisphere is strongest(weakest). No discernible Arctic impact is found over the North Atlantic and North Pacific region, which is consistent with predominantly southwesterly flow. An analysis of the flow-dependence of the linkages shows that anomalous northerly flow conditions increase the Arctic influence on midlatitude weather over the continents. Specifically, an anomalous northerly flow from the Kara Sea towards West Asia leads to cold surface temperature anomalies not only over West Asia but also over Eastern and Central Europe. Finally, the results of this study are discussed in the light of potential midlatitude benefits of improved Arctic prediction capabilities. | Tido SEMMLER Thomas JUNG Marta A.KASPER Soumia SERRAR | 2018 | Advances in Atmospheric Sciences2018，35，1： | 1 |
| 18 | Sequential extract ion of heavy metals during composting of sew age sludge 显示文摘 | Soumia A Mohamed H Georges M | 2005 | Chemosphere2005，59，： | 1 |
| 19 | 高效液相色谱-质谱联用分析磷酸可待因及其精氨酸布洛芬复方中的有关物质(英文)显示文摘建立了高效液相色谱法检查磷酸可待因、磷酸可待因与精氨酸布洛芬复方的有关物质,并采用HPLC-TOF/MS和LC-MS/MS对磷酸可待因及两种复方药物产生的降解产物进行了结构鉴定。色谱柱为C18(250 mm×4.6 mm,5μm),柱温50°C,流速1 mL/min,检测波长245 nm,醋酸铵(0.04 M,用醋酸调pH 6.0)–乙腈(92:8,v/v)为流动相A,乙腈为流动相B,梯度洗脱。磷酸可待因在氧化条件下很不稳定产生了两个N-氧可待因异构体,在碱性破坏中得到了新的杂质,其结构可能为6-羟基-3-甲氧基-17-甲基-7,8-双脱氢吗啡-5-醇。在酸性破坏条件下磷酸可待因和精氨酸布洛芬可发生酯化反应。所建立的HPLC方法灵敏、准确,可有效测定磷酸可待因、磷酸可待因与精氨酸布洛芬复方的有关物质,并对产生的降解产物进行了结构鉴定,为更好的控制磷酸可待因与磷酸可待因复方药物的质量提供了依据。 | Soumia Cheddah 杭太俊 | 2014 | Journal of Chinese Pharmaceutical Sciences2014，23，10： | 1 |
| 20 | Residence time of water and phytoplankton biomass in coral reeflagoons显示文摘 | Delesane B Soumia A | | 0，，7： | 1 |
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