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| 1 | Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells显示文摘人的 pluripotent 干细胞(hPSC ) 导出 endothelial 系细胞组成有希望的来源因为在这个竞技场的治疗学的 revascularization,而是进步被临床上可伸缩的区别协议和在移植之上与宿主发行量集成的一个功能的容器网络的低效的形成的缺乏妨碍了。在绿的地方,使用一根人的胚胎的干细胞记者房间线荧光灯蛋白质表示被 endothelial 驾驶房间特定的 VE-cadherin (VEC ) 倡导者,我们为 > 屏蔽了;60 bioactive 将支持 endothelial 区别,并且发现 BMP4 和 GSK-3β 的那个政府的小分子;在有 VEGF 的一个早阶段和处理的禁止者 -- 在一个以后的阶段表明小径的槽口的 A 和抑制在六天以内导致了 hPSCs 的有效区别到 endothelial 系。这条顺序的途径产生了 >到 endothelial 房间(EC ) 的 hPSCs 的50%变换,明确地 VEC + CD31 + CD34 + CD14 − KDR 在 endothelial 系房间之中展出了更高的 angiogenic 和 clonogenic 增长潜力的高 endothelial 祖先( EP )。药品的抑制或基因槽口发信号击倒,在有 VEGF 的联合 -- 一个处理,经由 KDR + mesodermal 先锋和对成熟 EC 的 EP 的变换的封锁导致了 EP 的有效形成。当移植了进 immunocompromised 老鼠时,产生 EP 成功地与吻合在 vivo 形成了功能的毛状的容器到主人容器。在我们的协议表明电路的这 VEGF-A-Notch 的操作由 12- 导致导出 hPSC 的 EP 的快速的大规模生产到 20 褶层对当前的方法,它可以与与成熟 EC 相比形成能力的优异容器为再生 vascularization 用作一张吸引人的房间人口。 | Makoto Sahara Emil M Hansson Oliver Wemet Kathy O Lui Daniela Spater Kenneth R Chien | 2014 | Cell Research2014，24，7： | 10 |
| 2 | A HCN4+ car- diomyogenic progenitor derived from the first heart field and human pluripotent stem cells 显示文摘 | Spater D Abramczuk MK Buac K | 2013 | Nat Cell Biol2013，15，9： | 1 |
| 3 | Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis显示文摘Tissue (re)vascularization strategies face various challenges, as therapeutic cells do not survive long enough in situ, while the administration of pro-angiogenic factors is hampered by fast clearance and insufficient ability to emulate complex spatiotemporal signaling. Here, we propose to address these limitations by engineering a functional biomaterial capable of capturing and concentrating the pro-angiogenic activities of mesenchymal stem cells (MSCs). In particular, dextran sulfate, a high molecular weight sulfated glucose polymer, supplemented to MSC cul-tures, interacts with MSC-derived extracellular matrix (ECM) components and facilitates their co-assembly and accumulation in the pericellular space. Upon decellularization, the resulting dextran sulfate-ECM hybrid material can be processed into MIcroparticles of SOlidified Secretome (MIPSOS). The insoluble format of MIPSOS protects protein components from degradation, while facilitating their sustained release. Proteomic analysis demonstrates that MIPSOS are highly enriched in pro-angiogenic factors, resulting in an enhanced pro-angiogenic bioactivity when compared to naïve MSC-derived ECM (cECM). Consequently, intravital microscopy of full-thickness skin wounds treated with MIPSOS demonstrates accelerated revascularization and healing, far superior to the ther-apeutic potential of cECM. Hence, the microparticle-based solidified stem cell secretome provides a promising platform to address major limitations of current therapeutic angiogenesis approaches. | Thomas Spater Marisa Assunçao Kwok Keung Lit Guidong Gong Xiaoling Wang Yi-Yun Chen Ying Rao Yucong Li Chi Him Kendrick Yiu Matthias W.Laschke Michael D.Menger Dan Wang Rocky S.Tuan Kay-Hooi Khoo Michael Raghunath Junling Guo Anna Blocki | 2022 | Bioactive Materials2022，7，11： | 1 |
| 4 | Canonical Wnt/beta-catenin signaling prevents osteoblats from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Dev Cell2005，8，5： | 1 |
| 5 | Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Dev Cell2005，8，5： | 1 |
| 6 | Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | | 0，，5： | 1 |
| 7 | Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes 显示文摘 | Hill T P Spater D Taketo M M | 2005 | Dev Cell2005，8，5： | 1 |
| 8 | Canonical Wnt/betacatenin signaling prevents osteoblasts from differenting into chongrocytes 显示文摘 | Hill T R Spater D Taketo M | 2005 | Dev Cell2005，8，： | 1 |
| 9 | Wnt9a signaling is requiredfor joint integrity and regulation of Ihh during chondrogenesis显示文摘 | Spater D Hill TP O'Sullivan RJ | 2006 | De-velopment2006，133，15： | 1 |
| 10 | Wnt9a signaling is required for joint integrity and regulation of Ihh during chondrogenesis 显示文摘 | Spater D Hill TP Gruber M | 2006 | De- velopment2006，133，： | 1 |
| 11 | Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Dev Cell2005，8，5： | 1 |
| 12 | Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill T P Spater D Taketo M M | 2005 | Dev Cell2005，8，5： | 1 |
| 13 | Canonical Win/beta- catenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Developmental Cell2005，8，5： | 1 |
| 14 | Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Dev Cell2005，8，5： | 1 |
| 15 | Canonical Wnt/betacatenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Dev Cell2005，8，5： | 1 |
| 16 | Canonical Wnt/β- cantenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | HILL TP SPATER D TAKETO MM | 2005 | Dev Cell2005，8，5： | 1 |
| 17 | Canonical Wnt/beta- catenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Developmental Cell2005，8，5： | 1 |
| 18 | Canonical Wnt/beta-cate- nin signaling prevents osteoblasts from differentiating into chondro- cytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Dev Cell2005，8，5： | 1 |
| 19 | Canonical Wnt/betacatenin signaling prevents osteoblasts from differentiating into chondrocytes显示文摘 | Hill TP Spater D Taketo MM | 2005 | Dev Cell2005，8，5： | 1 |
| 20 | Canonical Wnt/beta-catenin signaling prevents osteoblasts from differentiating into chondro- cytes 显示文摘 | Hill TP Spater D Taketo MM | 2005 | Dev Cell2005，8，5： | 1 |
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