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| 1 | 脂多糖对大鼠乳腺上皮细胞酪蛋白基因表达的影响显示文摘本试验旨在探讨脂多糖(LPS)对大鼠乳腺上皮细胞中αs1-酪蛋白(CSN1S1)、β-酪蛋白(CSN2)和乳清蛋白(α-LA)3种主要酪蛋白基因表达的影响。采用单因素试验设计,用实时荧光定量PCR(RT-PCR)法研究在催乳素诱导作用下,0、100、500、1000、5000和25000 ng/mL的LPS作用于HC11细胞3和24 h对酪蛋白基因(CSN1S1、CSN2和α-LA)、葡萄糖转运因子1(GLUT1)、葡萄糖转运因子8(GLUT8)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)基因表达的影响。结果表明:不同剂量、不同作用时间的LPS作用于HC11细胞,均显著抑制了CSN2基因的表达;作用3 h时,500~1000 ng/mL的LPS显著增加了α-LA基因的表达量(P<0.05),作用24 h时,所有剂量的LPS均显著抑制了α-LA基因的表达(P<0.05);500~25000 ng/mL的LPS作用3 h时和所有剂量的LPS作用24 h时均显著增加了CSN1S1基因的表达量(P<0.05);LPS显著增加了TNF-α基因的表达量(P<0.05);1000 ng/mL的LPS作用3 h时和100~25000 ng/mL的LPS作用24 h时,均显著增加了IL-6基因的表达量(P<0.05);500~25000 ng/mL的LPS作用3 h时显著增加了IL-1β基因的表达量(P<0.05);从时间效应来看,总体趋势是3种细胞因子(IL-6、IL-1β、TNF-α)在作用24 h时的基因表达量均高于作用3 h时。大于5000 ng/mL的LPS作用3 h时显著增加了GLUT1基因的表达量(P<0.05),所有剂量LPS均显著抑制了GLUT8基因的表达(P<0.05)。综上,在大鼠乳腺上皮细胞中,LPS对不同酪蛋白基因的表达有不同的影响,LPS能抑制CSN2和α-LA基因的表达,却能增强CSN1S1基因的表达。 | 田青 SPITZER A J 赵凤启 | 2020 | 动物营养学报2020，32，7： | 4 |
| 2 | Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4显示文摘 | Visintin A Mazzoni A Spitzer J A | 2001 | Proc Natl Acad Sci U S A2001，98，12： | 2 |
| 3 | Histamine regulates cytokine production in maturing dendritic cells,resulting in altered T cell polarization显示文摘 | Mazzoni A Young HA Spitzer JH | 2001 | J Clin Invest2001，108，12： | 1 |
| 4 | Toll-like receptorl inhibits Toll-like receptor 4 signaling in endothelis cells显示文摘 | Spitzer J H Visintin A Mazzoni A | 2002 | Eur J Immunol2002，32，4： | 1 |
| 5 | The influence of storage conditions on flavour changes in human milk显示文摘 | SPITZER J DOUCET S BUETTNER A | | 0，，： | 1 |
| 6 | Regulation of Toll-like receptors in human monoeytes and dendritic cells显示文摘 | Visintin A Mazzoni A Spitzer JH | 2001 | J Immunol2001，166，1： | 1 |
| 7 | Antirhino/entero- viral vinylacetylene benzimidazoles : a study of their activity and oral plasma levels in mice显示文摘 | Tebbe M J Spitzer W A Victor F | 1997 | J Med Chem1997，40，24： | 1 |
| 8 | secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4显示文摘 | Visintin A Mazzoni A Spitzer JA | 2001 | Proc Natl Acad Sci USA2001，98，12： | 1 |
| 9 | Perivascular human endometrial mesenchymal stem cells express pathways relevant to self-renewal,lineage specification,and functional phenotype显示文摘 | Spitzer TL Rojas A Zelenko Z | | 0，，02： | 1 |
| 10 | Regulation of Toll-like receptors in human monocytes and dendritic cells显示文摘 | Visintin A Mazzoni A Spitzer JH | 2001 | J Immunol2001，166，： | 1 |
| 11 | Prolonged vasospaam in migrainedetected by noninvasive transcraniai doppler ultrasound显示文摘 | Thie A Spitzer K Lachenmayer L | 1988 | Headache1988，28，： | 1 |
| 12 | Measuring the quality of life of cancer patients: a concise QL-tndex for use by physicians显示文摘 | Spitzer WO Dobson A J Hall J | 2008 | J Chronic Dis2008，34，12： | 1 |
| 13 | Sarcoplasmic reticulum and Na^+/Ca^2+ exchanger function during early and late relaxation in ventricular myocytes显示文摘 | Yao A Matsui H Spitzer KW | 1997 | Am J Physiol1997，273，6： | 1 |
| 14 | Partial 1iquid ventilation with perflubron in premature infants with severe respiratory distress syndrome显示文摘 | Spitzer A R Lipsky C L | 1997 | Clin Pediatr (Phila)1997，36，3： | 1 |
| 15 | Thyrotoxicosis with post- treatment hypothyroidism in a patient with acute suppurative thyroiditis due to porphyromonas 显示文摘 | SPITZER M ALEXANIAN S FARWELL A P | 2012 | Thyroid2012，22，1： | 1 |
| 16 | Antiproliferative and cytotoxic properties of bevacizumab on different ocular cells 显示文摘 | Spitzer MS Wallenfels-Thilo B Sierra A | 2006 | Br J Ophthalmol2006，90，10： | 1 |
| 17 | The visible human male: a technical report显示文摘 | Spitzer V Ackerman M J Scherizinger A L | 1996 | J Am Med Inform Assoc1996，3，2： | 1 |
| 18 | Toll-like receptor inhibits Toll-like receptor 4 signaling in endothelial cells显示文摘 | Spitzer JH Visintin A Mazzoni A | 2002 | Eur J Immunol2002，32，： | 1 |
| 19 | Conservative treatment of cervical pregnancy by curettage and local prostaglandin injection 显示文摘 | Spitzer D Steiner H Graf A | 1997 | Hum Reprod1997，12，4： | 1 |
| 20 | Mitochondfial dysfunction is a primary event in glutamate neurotoxicity显示文摘 | Schinder A F Olson E C Spitzer N C | | 0，，19： | 1 |
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