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| 1 | Application of Cydia pomonella expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth显示文摘幼鳕蛾, Cydia pomonella,是在世界上的梨果水果的最重要的害虫之一,然而,分子的遗传和这个昆虫遗体的生理学糟糕理解。8341 个表示顺序标签的一个联合集会从八个织物特定的 cDNA 图书馆定序的 Roche 454 GS-FLX 被产生。通常认为的化学感应的蛋白质(12 ) 和有气味的东西绑定蛋白质(OBP )(18 ) 被注解,它包括了三通常认为的一般 OBP (GOBP ) ,一个人典型地非常为另外的鳞翅目报导了。为了推进,描绘 CpomGOBPs,我们克隆他们的抄本的 cDNA 拷贝并且在各种各样的纸巾决定了他们的表示模式。为 CpomGOBP1 698nt 抄本克隆并且定序导致了编码区域的 163 氨基酸的预言,并且随后的 RT-PCR 显示抄本主要在天线和 mouthparts 被表示。CpomGOBP3 抄本主要是的 702 nt (169 氨基酸) 在天线, mouthparts,和女腹部表示了的 CpomGOBP2 和小说付小费给的 1289 nt (160 氨基酸) 。这些结果显示定序的那下一代为兴趣的新奇抄本的鉴定是有用的,并且那幼鳕蛾表示为 GOBP 亚科的一个新成员编码的一个抄本。 | Stephen F. Garczynski Brad S. Coates Thomas R. Unruh Scott Schaeffer Derick Jiwan Tyson Koepke Amit Dhingra | 2013 | Insect Science2013，20，5： | 6 |
| 2 | Centralized versus decentralized provision of local public goods: a political economy approach显示文摘 | Timothy Besley Stephen Coate | 2003 | Journal of Public Economics2003，，12： | 2 |
| 3 | Group lending, repayment incentives and social collateral显示文摘 | Timothy Besley Stephen Coate | 1995 | Journal of Development Economics1995，，1： | 2 |
| 4 | Porphyromonas gingivalis Resistance to Polymyxin B Is Determined by the Lipid A 4’-Phosphatase, PGN_0524显示文摘Aim To elucidate the genetic basis for the pronounced resistance that the oral pathogen, Porphyromonas gingivalis (P. gingivalis), exhibits towards the cationic antimicrobial peptide, polymyxin B. Methodology A genetic screen of P. gingivalis clones generated by a Tn4400’-based random insertion mutagenesis strategy was performed to identify bacteria harboring novel genetic mutations that render P. gingivalis susceptible to killing by the cationic antimicrobial peptide, polymyxin B (PMB, 50 μg-mL-1). Results P. gingivalis (ATCC 33277) is unusually resistant to the cationic antimicrobial peptide, PMB at relatively high concentrations (200 μg-mL-1). Approximately 2,700 independent Tn4400’-derived mutants of P. gingivalis were examined for increased sensitivity to PMB killing at a relatively low dose (50 μg-mL-1). A single PMB-sensitive mutant was obtained in this phenotypic screen. We determined that the Tn4400’ transposon was integrated into the gene encoding the lipid A 4’-phosphatase, PGN_0524, demonstrating that this insertion event was responsible for its increased susceptibility of this clone to PMB-dependent killing. The resulting mutant strain, designated 0524-Tn4400’, was highly sensitive to PMB killing relative to wild-type P. gingivalis, and exhibited the same sensitivity as the previously characterized strain, 0524KO, which bears a genetically engineered deletion in the PGN_0524 locus. Positive ion mass spectrometric structural (MALDI-TOF MS) analyses revealed that lipid A isolates from 0524-Tn4400’ and 0524KO strains displayed strikingly similar MALDI-TOF MS spectra that were substantially different from the wildtype P. gingivalis lipid A spectrum. Finally, intact 0524- Tn4400’ and 0524KO mutant bacteria, as well as their corresponding LPS isolates, were significantly more potent in stimulating Toll-like receptor 4 (TLR4)-dependent E-selectin expression in human endothelial cells relative to intact wild-type P. gingivalis or its corresponding LPS isolate. Conclusion The combined molecular evidence provided in this report suggests that PGN_0524, a lipid A 4’-phosphatase, is the sole genetic element conferring the ability of the periodontopathogen, P. gingivalis, to evade the killing activity of cationic antimicrobial peptides, such as PMB. These data strongly implicate PGN_0524 as a critical virulence factor for the ability of P. gingivalis to evade front-line host innate defenses that are dependent upon cationic antimicrobial peptide activity and TLR4 sensing. | Stephen R. Coats Thao T. To Sumita Jain Pamela H. Braham Richard P. Darveau | 2009 | International Journal of Oral Science2009，1，3： | 2 |
| 5 | Why HIV Infections Have Increased Among Men Who Have Sex with Men and What to Do About It: Findings from California Focus Groups显示文摘 | Stephen F. Morin Karen Vernon John (Jay) Harcourt Wayne T. Steward Jonathan Volk Thomas H. Riess Torsten B. Neilands Marisa McLaughlin Thomas J. Coates | 2003 | AIDS and Behavior2003，，4： | 1 |
| 6 | Reciprocity Without Commitment,Characterization and Performance of Informal Risk-sharing arrangements显示文摘 | Coate Stephen and Martin Ravallion | 1993 | Journal of Development Economics1993，，40： | 1 |
| 7 | Group Lending, Repayment Incentives and Social Collateral显示文摘 | Besley Timothy Stephen Coate | | 0，，04： | 1 |
| 8 | Elected versus appointed regulators : theory and evidence显示文摘 | Besley Timothy Stephen Coate | 2003 | Journal of The European Economic Association2003，，5： | 1 |
| 9 | Lobbying and Welfare in a Representative Democracy显示文摘 | Besley Timothy Coate Stephen | 2001 | Review of Economic Studies Blackwell Publishing2001，68，1： | 1 |
| 10 | The Economics of Rotating Savings and Credit Association显示文摘 | BESLEY Timothy COATE Stephen LOURY Glenn | | 0，，83： | 1 |
| 11 | Welfare Economics and the Evaluation of Policy Changes显示文摘 | STEPHEN COATE | 1997 | PIER Working Paper1997，40，： | 1 |
| 12 | Reciprocity Without Commitment:Characterization and Performance of Informal Risk - sharing Arrangements 显示文摘 | Coate Stephen Martin Ravallion | 1993 | Journal of Development Economics1993，，40： | 1 |
| 13 | The Design of Income Maintenance Programmes,Review of Economic Studies显示文摘 | Besley Timothy & Coate Stephen | 1995 | Wiley Blackwell1995，62，2： | 1 |
| 14 | Increased Neurotrophin Production in a Penicillium chrysogenum-Induced Allergic Asthma Model in Mice显示文摘 | Yong Joo Chung Aimen Farraj Najwa Haykal Coates Stephen H. Gavett Marsha D. W. Ward | 2007 | Journal of Toxicology and Environmental Health, Part A2007，，： | 1 |
| 15 | Group lending,repayment incentives and social Collateral显示文摘 | Timothy Besley Stephen Coate | | 0，，： | 1 |
| 16 | Work{are versus Welfare: Incentive Arguments for Work Requirements in Poverty- Alleviation Programs 显示文摘 | Timothy Besley Stephen Coate | 1992 | American Economic Review1992，82，： | 1 |
| 17 | The Eco- nomics of Rotating Savings and Credit Associations 显示文摘 | Timothy Besley Stephen Coate and Glenn Loury | 1993 | The American Economic Review1993，83，4： | 1 |
| 18 | The economics of rotating savings and credit associations 显示文摘 | Besley Timothy Stephen Coate Glenn Loury | 1993 | American Economic Review1993，83，4： | 1 |
| 19 | Policy Persistence显示文摘 | Coate Stephen Stephen E Morris | | 0，，05： | 1 |
| 20 | Proinflammatory cytokines oppose opioid-induced acute and chronic analgesia显示文摘 | Mark R. Hutchinson Benjamen D. Coats Susannah S. Lewis Yingning Zhang David B. Sprunger Niloofar Rezvani Eric M. Baker Brian M. Jekich Julie L. Wieseler Andrew A. Somogyi David Martin Stephen Poole Charles M. Judd Steven F. Maier Linda R. Watkins | 2008 | Brain Behavior and Immunity2008，，8： | 1 |
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