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| 1 | 荧光多重实时PCR检测单纯疱疹病毒显示文摘目的建立单纯疱疹病毒的荧光多重实时PCR检测及分型技术,为生殖器疱疹的诊断及病毒分型提供快捷、敏感、特异的方法.方法以细胞培养法为对照,用Smart Cycler System作为实验平台,以DNA探针荧光标记技术建立荧光多重实时PCR,用于检测生殖器疱疹患者临床标本中的1型和2型单纯疱疹病毒(HSV1、HSV2)DNA.结果荧光多重实时PCR检测HSV1和HSV2 DNA的敏感性和特异性均为100%,可检测出低至1~5拷贝的HSV DNA,30 min左右即可完成整个检测过程,而细胞培养的敏感性和特异性分别为70.10%和100%.结论基于Smart Cycler System的荧光多重实时PCR是一种易于操作、扩增效率高、封闭式的核酸扩增技术,可用一次PCR反应就能快速、敏感、准确地对临床标本中的单纯疱疹病毒进行检测和分型. | 赖维 Cheng-Yen Chen 苏向阳 黄怀球 张玉清 Ron C Ballard Stephen A Morse | 2004 | 中华皮肤科杂志2004，37，5： | 6 |
| 2 | Campylobacter concisus and inflammatory bowel disease显示文摘Investigation of the possible role of Campylobacter concisus(C. concisus) in inflammatory bowel disease(IBD) is an emerging research area. Despite the association found between C. concisus and IBD, it has been difficult to explain how C. concisus, a bacterium that is commonly present in the human oral cavity, may contribute to the development of enteric diseases. The evidence presented in this review shows that some C. concisus strains in the oral cavity acquired zonula occludens toxin(zot) gene from a virus(prophage) and that C. concisus Zot shares conserved motifs with both Vibrio cholerae Zot receptor binding domain and human zonulin receptor binding domain. Both Vibrio cholerae Zot and human zonulin are known to increase intestinal permeability by affecting the tight junctions. Increased intestinal permeability is a feature of IBD. Based on these data, we propose that a primary barrier function defect caused by C. concisus Zot is a mechanism by which zot-positive C. concisus strains may trigger the onset and relapse of IBD. | Li Zhang Hoyul Lee Michael C Grimm Stephen M Riordan Andrew S Day Daniel A Lemberg | 2014 | World Journal of Gastroenterology2014，20，5： | 4 |
| 3 | Cancer-related inflammation and treatment effectiveness显示文摘 | Connie I Diakos Kellie A Charles Donald C McMillan Stephen J Clarke | 2014 | Lancet Oncology2014，，： | 3 |
| 4 | Identification of telmisartan as a unique angiotensin Ⅱ receptor antagonist with selective ppargamma- modulating activity 显示文摘 | Stephen C Benson Harrihar A Pershadsingh Christopher I Ho | 2004 | Hypertension2004，43，： | 1 |
| 5 | Haplotype variation and linkage disequilibrium in 313 human genes显示文摘 | Stephens J C Schneider J A Tanguay D A | 2001 | Science2001，293，： | 1 |
| 6 | Repopu- lation of gamma-irradiated Lewis lung carcinoma by malig- nant cells and host macrophage progenitors显示文摘 | STEPHENS T C CURRIE G A PEACOCK J H | 1978 | Br J Cancer1978，38，5： | 1 |
| 7 | Mold Contamination and Air Handling Units显示文摘 | Stephen C Wilson Robert N Palmatier Larysa A Andriychuk | 2007 | Journal of Occupational and Environmental Hygiene2007，4，7： | 1 |
| 8 | Chemokine receptors CCR6 and CXCR3 are necessary for CD4 + T cell mediated ocular surface disease in experimen- tal dry eye disease显示文摘 | TERRY G NIRAL B EUGENE A STEPHEN C CINTIA S | 2013 | PLoS One2013，8，78： | 1 |
| 9 | Haplotype Variation and Linkage Disequilibrium in 313 Human Genes显示文摘 | STEPHENS J C SCHNEIDER J A DEBRA A | 2001 | Sci2001，，293： | 1 |
| 10 | An intertemporal general equilibrium model of asset prices and theory of the term struc- ture of interest rates显示文摘 | JOHN C JONATHAN E STEPHEN A | 1985 | Econometrica1985，53，2： | 1 |
| 11 | Quantitative experimental study of shear stresses and mixing in progressive flow regimes within annular-flow bioreactors显示文摘 | Stephen J C Richard A B | 2004 | Chemical Engineering Science2004，59，： | 1 |
| 12 | Human CD4^+CD25^+ thymocytes and peripheral T cells have immune suppressive activity in vitro显示文摘 | Stephens L A Mottet C Mason D | 2001 | Eur J Immunol2001，31，4： | 1 |
| 13 | Identification of telmisartan as a unique angiotensin II receptor antagonist with selective PPARY modulating activity 显示文摘 | Stephen C Harrihar A Christopher I | 2004 | Hypertention2004，43，： | 1 |
| 14 | Investigation of benzene oxide in bone marrow and other tissues of F344 rats following metabolism of benzene in vitro and in vivo 显示文摘 | Andrew B L Karen YO C Suramya W Thomas A M Bernard T G Stephen M R | 1998 | Chemical Research in Toxicology1998，11，4： | 1 |
| 15 | Long-termexposure of plaque to zinc citrate 显示文摘 | Jones C L Stephen K W Ritchie J A | 1988 | Caries Res1988，22，2： | 1 |
| 16 | Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars 显示文摘 | Cavanagh CS Chao S Wang BE Huang BE Stephen S Kiani S Forrest K Saintenac C Brown-Guedira GL Akhunova A See D Bai G Pumphrey M Tomar L Wong D Kong S Reynolds M da Silva ML Bockelman H Talbert L Anderson JA Dreisigacker S Baenziger S Carter A Korzun V Morrell PL Dubcovsky J Morell MK Sorrells ME Hayden MJ Akhunov E | 2013 | PNAS2013，110，： | 1 |
| 17 | Option pricing:a simplified approach显示文摘 | Ross Stephen A Stein Mark R | 1979 | Journal of Financial Eonomics1979，7，5： | 1 |
| 18 | A com- parison of surface water natural organic matter in raw filtered wa- ter samples, XAD, and reverse osmosis isolates 显示文摘 | PATRICIA A M M1VHAEL J P STEPHEN E C | 2002 | Water Re- search2002，36，： | 1 |
| 19 | Conceptual and methodological isses in consumer satisfaction research显示文摘 | LaTour Stephen A Nancy C Peat | 1979 | Advances in Consumer Research1979，6，： | 1 |
| 20 | An Intertemporal General Equilibrium Model of Asset Prices显示文摘 | Cox John C Jonathan E Ingersoll Stephen A Ross | 1985 | Econometrica1985，53，： | 1 |
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