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| 1 | 两步水解法制备玉米抗氧化活性肽及产物的稳定性显示文摘采用麦芽粉和碱性蛋白酶Alcalase对玉米醇溶蛋白进行两步水解制备玉米肽,以水解度、可溶性蛋白含量和抗氧化活性为指标,对第二步Alcalase的酶解条件进行优化,以确定制备玉米抗氧化肽的最适条件。另外,对玉米抗氧化肽的稳定性进行了研究。结果表明:制备玉米抗氧化肽的最适条件为:第一步麦芽粉水解,在底物浓度10%、pH 5.5、温度50℃、酶底比30%条件下水解3 h;第二步Alcalase酶解,在底物浓度10%、pH 8.5、温度60℃、酶底比0.75%条件下水解6 h。在此条件下,水解度、可溶性蛋白含量、羟基自由基清除率及亚铁离子螯合率分别为24.53%、33.43 mg/mL、30.59%、38.32%,分子量主要分布在300~6500 u(92.51%)范围内。玉米抗氧化肽具有热及贮藏稳定性,在中性和碱性条件下稳定,但不耐受酸性条件(pH 3~5)。 | 王晓杰 刘晓兰 曲悦 夏春荣 Stanislav Sukhikh Anna Andreyeva | 2020 | 中国粮油学报2020，35，12： | 10 |
| 2 | 两步水解法制备玉米-大豆复合抗氧化肽及其稳定性研究显示文摘采用麦芽粉和碱性蛋白酶Alcalase两步水解玉米醇溶蛋白和大豆分离蛋白的混合物(质量比1∶1)制备复合抗氧化肽,以水解度、可溶性蛋白含量和抗氧化活性为指标,对第二步Alcalase的水解条件进行优化,确定两步水解法制备复合抗氧化肽的最适条件。另外,对复合抗氧化肽相对分子质量分布、稳定性和氨基酸组成进行了研究。结果表明,制备复合抗氧化肽的最适条件为在底物质量分数10%、pH 5.5、50℃、酶(麦芽粉)底质量比30%条件下水解3 h后,再在底物质量分数10%、pH 8.5、60℃、酶(Alcalase)底质量比0.75%条件下继续水解3 h。在最适条件下,水解度、可溶性蛋白含量、羟自由基清除率和亚铁离子螯合率分别为36.32%、36.83 mg/mL、40.01%和86.83%,相对分子质量主要分布在300~6 500 Da (含量92.44%)范围内,必需氨基酸指数(EAAI)值为8.02(n=9),属于优质蛋白源。复合抗氧化肽具有热稳定性,在中性及碱性条件下稳定,但不耐受酸性(pH 3~5)、4℃贮存和反复冻融的加工条件。 | 王晓杰 刘晓兰 曲悦 夏春荣 SUKHIKH Stanislav ANDREYEVA Anna | 2021 | 中国油脂2021，46，3： | 3 |
| 3 | Multipotent stromal cells stimulate liver regeneration by influencing the macrophage polarization in rat显示文摘AIM To investigate the influence of the umbilical cordderived multipotent stromal cells(MSCs) on recovery of the liver after the subtotal resection, that is, removal of 80% of the organ mass, a renowned model of the small-for-size liver remnant syndrome. METHODS The MSCs were obtained from the intervascular tissue of umbilical cords, dissected from rat fetuses, by the explant culture technique. The vital labeling of MSCs with РКН26 was carried out on the 3 rd passage. The subtotal resection was performed on male Sprague-Dawley rats. The experimental group animals received a transplant 106 MSCs infused into the spleen. Hepatocyte proliferation was assessed by counting of either mitotic figures or Ki67-positive cells in microscopic images. MSC differentiation was assessed with antibodies to hepatocyte-specific marker cytokeratin 18(CK18), cholangiocyte-specific protein CK19, smooth muscle cell-specific protein α-SMA, the endothelial cell marker CD31, or the active fibroblast marker FAPα. Total macrophages of the liver were selectively stained in cryosections incubated with antiCD68 antibodies(1:100, Abcam), while the M2 a and M2 c macrophage populations were selectively stained with anti-CD206 antibodies. Expression of interleukin and growth factor genes was evaluated with PCR-RT.RESULTS Intrasplenic allogeneic transplantation of the umbilical cord-derived multipotent stromal cells stimulates reparative processes within the residual liver tissue after subtotal resection(removal of 80% of the organ mass), as indicated by increased rates of hepatocyte proliferation and accelerated organ mass recovery. These effects may result from paracrine influence of the transplanted cells on the resident macrophage population of the liver. The transplantation favors polarization of macrophages to M2 phenotype(the M2-polarized macrophages specifically express CD206; they are known to suppress inflammation and support tissue repair). No differentiation of the transplanted cells into any of the liver cell types have been observed in the study.CONCLUSION We found no direct evidence for the paracrine effect of MSCs on liver regeneration after the subtotal liver resection in rats. However, the paracrine mechanism of the therapeutic activity of transplanted MSC is indirectly indicated by a decrease in the total number of CD68 + macrophages and an increase in the proportion of M2 pro-repair macrophages in the regenerating liver as compared to animals in which the transplantation was only mimicked. | Andrey Elchaninov Timur Fatkhudinov Natalia Usman Irina Arutyunyan Andrey Makarov Anastasia Lokhonina Irina Eremina Viktor Surovtsev Dmitry Goldshtein Galina Bolshakova Valeria Glinkina Gennady Sukhikh | 2018 | World Journal of Hepatology2018，10，2： | 2 |
| 4 | 大豆蛋白-黄芩素的结合机制及蛋白构象和功能变化显示文摘探索大豆蛋白组分中β-伴大豆蛋白(7S)/大豆蛋白(11S)与黄芩素的结合机制,考察复合物构象及功能特性的变化。傅里叶变换红外光谱表明黄芩素诱导蛋白的β-折叠转化为α-螺旋和无规卷曲。内源荧光光谱证实了黄芩素的加入使7S、11S结构变得更紧密。黄芩素与蛋白的反应自发进行,并通过静态方式猝灭蛋白荧光。7S、11S蛋白分别通过氢键和疏水相互作用与黄芩素结合。分子对接结果表明,黄芩素对11S的亲和力高于7S。扫描电子显微镜显示了7S、11S与复合物的微观结构差异。此外,与黄芩素结合后,7S、11S的表面疏水性下降,热稳定性及其他功能特性提升。 | 闫馨月 贾亦佳 孙诗艳 张东蒙 耿梦洁 杨晋杰 Stanislav SUKHIKH Olga BABICH 齐宝坤 李杨 | 2023 | 食品科学2023，44，4： | 2 |
| 5 | Regenerative medicine of pancreatic islets显示文摘The pancreas became one of the first objects of regenerative medicine,since other possibilities of dealing with the pancreatic endocrine insufficiency were clearly exhausted.The number of people living with diabetes mellitus is currently approaching half a billion,hence the crucial relevance of new methods to stimulate regeneration of the insulin-secretingβ-cells of the islets of Langerhans.Natural restrictions on the islet regeneration are very tight;nevertheless,the islets are capable of physiological regeneration viaβ-cell self-replication,direct differentiation of multipotent progenitor cells and spontaneousα-toβ-orδ-toβ-cell conversion(trans-differentiation).The existing preclinical models ofβ-cell dysfunction or ablation(induced surgically,chemically or genetically)have significantly expanded our understanding of reparative regeneration of the islets and possible ways of its stimulation.The ultimate goal,sufficient level of functional activity ofβ-cells or their substitutes can be achieved by two prospective broad strategies:β-cell replacement andβ-cell regeneration.The“regeneration”strategy aims to maintain a preserved population ofβ-cells through in situ exposure to biologically active substances that improveβ-cell survival,replication and insulin secretion,or to evoke the intrinsic adaptive mechanisms triggering the spontaneous non-β-toβ-cell conversion.The“replacement”strategy implies transplantation ofβ-cells(as non-disintegrated pancreatic material or isolated donor islets)orβ-like cells obtained ex vivo from progenitors or mature somatic cells(for example,hepatocytes orα-cells)under the action of small-molecule inducers or by genetic modification.We believe that the huge volume of experimental and clinical studies will finally allow a safe and effective solution to a seemingly simple goal-restoration of the functionally activeβ-cells,the innermost hope of millions of people globally. | Irina V Arutyunyan Timur Kh Fatkhudinov Andrey V Makarov Andrey V Elchaninov Gennady T Sukhikh | 2020 | World Journal of Gastroenterology2020，26，22： | 2 |
| 6 | Application of the magnetic saturation method for eddy-current inspection of spent fuel elements from fast reactors显示文摘 | SUKHIKH AV | 2007 | Atomic Energy2007，102，2： | 1 |
| 7 | Application of the magnetic saturation method for eddy-current inspection of spent fuel elements from fast reactors显示文摘 | SUKHIKH A V | 2007 | Atomic Energy2007，102，2： | 1 |
| 8 | Serum activi-ty of matrix metalloproteinases-2 and-9显示文摘 | Soboleva G M Shurshalina A V Sukhikh G T et ol | 2006 | Bull Exp Biol Med2006，141，2： | 1 |
| 9 | Stem cell transplantation for treatment of liver diseases:from biological foundations to clinical experience (review)显示文摘 | SUKHIKH GT SHTIL AA | 2003 | Int J Mol Med2003，11，： | 1 |
| 10 | Transplantation of cultured human neural stem cells in rabbits with experimental laser-induced damage to the retina显示文摘 | Pak NV Podgornyi OV Sukhikh GT | 2004 | Bull Exp Biol Med2004，138，5： | 1 |
| 11 | Comparative analysis of differentiation and behavior of human neural and mesenchymal stem cells In Vitro and In Vivo显示文摘 | M. A. Aleksandrova G. T. Sukhikh R. K. Chailakhyan O. V. Podgornyi M. V. Marei R. A. Poltavtseva Yu. V. Gerasimov | 2006 | Bulletin of Experimental Biology and Medicine2006，，1： | 1 |
| 12 | Development of neural stem/progenitor cells from human brain by transplantation into the brains of adult rats显示文摘 | Aleksandrova MA Poltavtseva RA Revishchin AV Korochkin LI Sukhikh GT | 2004 | Neurosci Behav Physiol2004，34，7： | 1 |
| 13 | Immunomor- phological Characteristics of Endometrium in women with chronic endometritis显示文摘 | Sukhikh GT Shurshalina AV Veryasov VN | 2006 | Bulletin of Experimenta I Biology and Medicine2006，141，1： | 1 |
| 14 | Mesenchymal stem cells 显示文摘 | Sukhikh G T Malaitsev V V Bogdanova I M | 2002 | Bull Exp Bid Med2002，133，2： | 1 |
| 15 | Application of the magnetic saturation method for eddy-current inspection of spent fuel elements from fast reactors显示文摘 | Sukhikh A V Sagalov S S | | 0，，02： | 1 |
| 16 | Comparison of mesenchymal stem cells obtained from different human tissues显示文摘 | Musina RA Bekchanova ES Sukhikh GT | 2005 | Bull Exp Biol Med2005，139，： | 1 |
| 17 | Immunomorpho logical characteristics of endom etrium in women with chronic endom etrit is显示文摘 | Sukhikh GT Shursha AV Veryasov VN | 2006 | BunExo BiolMed2006，141，1： | 1 |
| 18 | Stress decrease in re-sistance of an organism to a tumor growth and its prevention bycentral inhibiting metabolites显示文摘 | Sukhikh GT Meerson FZ Mikhaleva II | 1984 | Dokl Akad Nauk USSR1984，278，3： | 1 |
| 19 | Antisperm antibodies detection by flow cytometry is affected by aggregation of antigen-antibody complexes on the surface of spermatozoa显示文摘 | Nikolaeva MA Kulakov Ⅵ Sukhikh GT | 2000 | Hum Reprod2000，15，12： | 1 |
| 20 | The obstetric history of preeclampsia as a further maternal cardiovascular risk factor显示文摘 | Sukhikh GT Vikhliaeva EM Kholin AM | 2009 | Ter Arkh2009，81，10： | 1 |
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