|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Establishment and characterization of a cholangiocarcinoma cell line (RMCCA-1) from a Thai patient显示文摘AIM: To establish and characterize a new cell line derived from peripheral cholangiocarcinoma of a Thai patient.METHODS: The peripheral cholangiocarcinoma specimen surgically obtained from the patient was aseptically processed by washing and mincing before culturing in Ham’s F12 medium containing 10% fetal bovine serum. After 3 mo, when the cell line has become homogeneous and stabilized, several features were investigated, including growth characteristics, immunofluorescence staining for cytokeratins, expression of tumor markers, chromosomal analysis by G-banding and multicolour fluorescence in situ hybridization (mFISH), in vitro migration and invasion characteristics. RESULTS: The RMCCA-1 cell line has been established. These cells proliferated as a monolayer with a population doubling time of 48 h. Immunofluorescence staining showed positive staining for human cytokeratin 7 and 19 verifying the biliary epithelial origin. RMCCA-1 secreted carbohydrate antigen 19-9 (CA19-9), but insignificant levels of carcinoembryonic antigen (CEA) and α-fetoprotein (AFP). Chromosome analysis identified aneuploidy karyotypes with a modal chromosome number of 59. RMCCA-1 exhibited a low level of in vitro invasiveness, but a high degree of motility. The cell line exhibited a significant number of chromosomal aberrations as shown by mFISH and G-banding methods.CONCLUSION: A new cell line derived from peripheral cholangiocarcinoma of a Thai patient has been established. This cell line shows a low level of in vitro invasiveness, but a high degree of motility. It will serve as a valuable tool for further studies on tumor biology, molecular pathogenesis, metastatic mechanism and response to therapeutic drugs of cholangiocarcinoma. | Panthip Rattanasinganchan Kawin Leelawat Sa-ard Treepongkaruna Chintana Tocharoentanaphol Somboon Subwongchareon Tuangporn Suthiphongchai Rutaiwan Tohtong | 2006 | World Journal of Gastroenterology2006，12，40： | 7 |
| 2 | High level of urokinase plasminogen activator contributes to cholangiocarcinoma invasion and metastasis显示文摘AIM: To investigate the role of urokinase plasminogen activator (uPA) in cholangiocarcinoma (CCA) invasion and its correlation with clinicopathological parameters. METHODS: uPA expression in CCA tissue was determined by immunohistochemistry. The level of uPA from two CCA cell lines (HuCCA-1 and KKU-M213) and a noncancer immortalized cholangiocyte cell line (H69) was monitored by plasminogen-gelatin zymography and western blotting, whereas that of plasminogen activator inhibitor type 1 (PAI-1) protein and uPA receptor (uPAR)mRNA was monitored by western blotting and quantitative real-time reverse transcriptase polymerase chain reaction, respectively. Two independent methods were employed to suppress uPA function: a synthetic uPA inhibitor (B428) and silencing of uPA gene expression using siRNA. In vitro invasion of the uPA-disrupted cells was assessed by Matrigel-coated Transwell assay. RESULTS: The immunohistochemical study showed that 75.3% (131/174) of CCA tissues expressed uPA. High uPA expression was correlated with lymphatic invasion and metastasis of CCA patients. Plasminogen-gelatin zymography of the conditioned media and cell-surface eluates showed that both CCA cell lines, but not H69, expressed both secreted and membrane-bound forms of uPA. Although the two CCA cell lines, HuCCA-1 and KKU-M213, expressed a relatively high level of uPA and uPAR, the latter exhibited a much lower degree of in vitro invasiveness, correlating with a high expression of PAI-1 in the latter, but not in the former. Suppressing uPA function with a specific uPA inhibitor, B428, or with siRNA against uPA reduced in vitro invasiveness of KKU-M213 cells, demonstrating the requirement for uPA in the invasiveness of CCA cells. Therefore, our in vivo and in vitro studies suggest that uPA is an important requirement for the invasion process of CCA. CONCLUSION: uPA expression correlates with lymphatic invasion and metastasis in vivo and is required for CCA cell invasion in vitro , suggesting its potential as a therapeutic target. | Parichut Thummarati Sitsom Wijitburaphat Aruna Prasopthum Apaporn Menakongka Banchob Sripa Rutaiwan Tohtong Tuangporn Suthiphongchai | 2012 | World Journal of Gastroenterology2012，18，3： | 4 |
| 3 | Ngal knockdown by sirna in human cholangiocarcinoma ceils suppressed invasion by reduc- ing ngal/mmp-9 complex formation 显示文摘 | Nuntagowat C Leelawat K Tohtong R | 2010 | Clin Exp Metastasis2010，27，5： | 1 |
| 4 | Anti-metastatic effects of curcusone B, a diterpene from Jatropha curcas L 显示文摘 | Muangman S Thipporowong M Tohtong R | 2005 | IN VIVO2005，19，1： | 1 |
| 5 | Anti-metastatic effects ofcurcusone B, a diterpene from Jatropha curcas 显示文摘 | Muangman S Thippomwong M Tohtong R | 2005 | In Vivo2005，19，1： | 1 |
| 6 | NGAL knockdown by siRNA in human cholangiocarcinoma cells suppressed invasion by reducing NGAL/MMP-9 complex formation显示文摘 | Nuntagowat C Leelawat K Tohtong R | 2010 | Clin Exp Metastasis2010，27，5： | 1 |
| 7 | Silencing of CD44 by siRNA suppressed invasion,migration and adhesion to matrix,but not secretion of MMPs,of cholangiocarcinoma cells显示文摘 | Pongcharoen P Jinawath A Tohtong R | 2011 | Clin Exp Metastasis2011，28，8： | 1 |
| 8 | Analysis of cDNAs encoding Drosophila melanogaster myosin light chain kinase显示文摘 | RUTAIWAN TOHTONG DEYRA RODRIGUEZ DAVID MAUGHAN AMANDA SIMCOX | 1997 | Journal of Muscle Research and Cell Motility1997，，1： | 1 |
| 9 | NGAL knockdown by siRNA in human eholangiocarcinoma cells suppressed invasion by reducing NGAL/MMP-9 complex formation 显示文摘 | Nuntagowat C Leelawat K Tohtong R | 2010 | Clin Pathol2010，27，5： | 1 |
| 10 | Impairment of muscle function caused by mutations of phosphorylation sites in myosin regulatory light chain 显示文摘 | Tohtong R Yamashita H Graham M | 1995 | Nature1995，374，： | 1 |
| 11 | NGAL knockdown by siRNA in human cholangiocarcinoma cells suppressed invasion by reducing NGAL/ MMP-9 complex formation显示文摘 | Nuntagowat C Leelawat K Tohtong R | 2010 | Clinical Experimen- tal Metastasis2010，27，5： | 1 |
| 12 | Role of mTOR inhibitor in cholangiocarcinoma cell progression显示文摘 | Penpak Moolthiya Rutaiwan Tohtong Siriporn Keeratichamroen Kawin Leelawat | 2014 | Oncology Letters2014，，3： | 1 |
| 13 | Role of mtor inhibitor in cholangiocarcinoma cell progression显示文摘 | Moolthiya P Tohtong R Keeratichamroen S | 2014 | Oncol Lett2014，7，3： | 1 |
| 14 | NGAL knockdown by siRNA in human cholangiocarcinoma cells suppressed inva- sion by reducing NGAL/MMP-9 complex fomation 显示文摘 | Nuntagowat C Leelawat K Tohtong R | 2010 | Clin Exp Metastasis2010，27，5： | 1 |
| 15 | Dysregulation of microRNA in cholangiocarcinoma identified through a meta-analysis of microRNA profiling显示文摘BACKGROUND In the past decades,the potential of microRNA(miRNA)in cancer diagnostics and prognostics has gained a lot of interests.In this study,a meta-analysis was conducted upon the pooled miRNA microarray data of cholangiocarcinoma(CCA).AIM To identify differentially expressed(DE)miRNAs and perform functional analyses in order to gain insights to understanding miRNA-target interactions involved in tumorigenesis pathways of CCA.METHODS Raw data from 8 CCA miRNA microarray datasets,consisting of 443 samples in total,were integrated and statistically analyzed to identify DE miRNAs via comparison of levels of miRNA expression between CCA and normal bile duct samples using t-tests(P<0.001).The 10-fold cross validation was performed in order to increase the robustness of the t-test results.Our data showed 70 up-regulated and 48 down-regulated miRNAs in CCA. GeneOntology and pathway enrichment analyses revealed that mRNA targets of DEmiRNAs were significantly involved in several biological processes. The mostprominent dysregulated pathways included phosphatidylinositol-3 kinases/Akt,mitogen-activated protein kinase and Ras signaling pathways.CONCLUSIONDE miRNAs found in our meta-analysis revealed dysregulation in major cancerpathways involved in the development of CCA. These results indicated thenecessity of understanding the miRNA-target interactions and the significance ofdysregulated miRNAs in terms of diagnostics and prognostics of cancers. | Somsak Likhitrattanapisal Supeecha Kumkate Pravech Ajawatanawong Kanokpan Wongprasert Rutaiwan Tohtong Tavan Janvilisri | 2020 | World Journal of Gastroenterology2020，26，29： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员