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| 1 | Developing single nucleotide polymorphism(SNP)markers from transcriptome sequences for identification of longan(Dimocarpus longan)germplasm显示文摘Longan(Dimocarpus longan Lour.)is an important tropical fruit tree crop.Accurate varietal identification is essential for germplasm management and breeding.Using longan transcriptome sequences from public databases,we developed single nucleotide polymorphism(SNP)markers;validated 60 SNPs in 50 longan germplasm accessions,including cultivated varieties and wild germplasm;and designated 25 SNP markers that unambiguously identified all tested longan varieties with high statistical rigor(P<0.0001).Multiple trees from the same clone were verified and off-type trees were identified.Diversity analysis revealed genetic relationships among analyzed accessions.Cultivated varieties differed significantly from wild populations(Fst=0.300;P<0.001),demonstrating untapped genetic diversity for germplasm conservation and utilization.Within cultivated varieties,apparent differences between varieties from China and those from Thailand and Hawaii indicated geographic patterns of genetic differentiation.These SNP markers provide a powerful tool to manage longan genetic resources and breeding,with accurate and efficient genotype identification. | Boyi Wang Hua-Wei Tan Wanping Fang Lyndel W Meinhardt Sue Mischke Tracie Matsumoto Dapeng Zhang | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Developing single nucleotide polymorphism markers for the identification of pineapple(Ananas comosus)germplasm显示文摘Pineapple(Ananas comosus[L.]Merr.)is the third most important tropical fruit in the world after banana and mango.As a crop with vegetative propagation,genetic redundancy is a major challenge for efficient genebank management and in breeding.Using expressed sequence tag and nucleotide sequences from public databases,we developed 213 single nucleotide polymorphism(SNP)markers and validated 96 SNPs by genotyping the United States Department of Agriculture-Agricultural Research Service pineapple germplasm collection,maintained in Hilo,Hawaii.The validation resulted in designation of a set of 57 polymorphic SNP markers that revealed a high rate of duplicates in this pineapple collection.Twenty-four groups of duplicates were detected,encompassing 130 of the total 170 A cosmos accessions.The results show that somatic mutation has been the main source of intra-cultivar variations in pineapple.Multivariate clustering and a model-based population stratification suggest that the modern pineapple cultivars are comprised of progenies that are derived from different wild Ananas botanical varieties.Parentage analysis further revealed that both A.comosus var.bracteatus and A.comosus var.ananassoides are likely progenitors of pineapple cultivars.However,the traditional classification of cultivated pineapple into horticultural groups(e.g.‘Cayenne’,‘Spanish’,‘Queen’)was not well supported by the present study.These SNP markers provide robust and universally comparable DNA fingerprints;thus,they can serve as an efficient genotyping tool to assist pineapple germplasm management,propagation of planting material,and pineapple cultivar protection.The high rate of genetic redundancy detected in this pineapple collection suggests the potential impact of applying this technology on other clonally propagated perennial crops. | Lin Zhou Tracie Matsumoto Hua-Wei Tan Lyndel W Meinhardt Sue Mischke Boyi Wang Dapeng Zhang | 2015 | Horticulture Research2015，2，1： | 2 |
| 3 | Carbon solubility in olivine:implications for upper-mantle evolution 显示文摘 | Tracy N T Green H W | 1987 | Geology1987，15，： | 2 |
| 4 | Effects of starchy sugary-2 and sugary sugary-2 endosperm on pericarp thickness in sweet and waxy corn显示文摘 | Schmidt D H Tracy W F | 1998 | Hort Science1998，23，5： | 1 |
| 5 | Effects of starchy-2 and sugary sugary-2endosperm on pericarp thickness in sweet corn显示文摘 | Schmidt D H Tracy W F | | 0，，05： | 1 |
| 6 | Fertility of the Population of Canada显示文摘 | Tracy W R | 1931 | Reprinted from Seventh Census of Canada1931，，2： | 1 |
| 7 | Microstructure evolution during FSW/FSP of high strength aluminum alloys 显示文摘 | Su Jian-Qing Tracy W Nelson Colin J Sterling | 2005 | Materials Science and Engineering A2005，405，： | 1 |
| 8 | Effect of surfactant and particle size reduction on hydrolysis of deinking sludge and nonrecyclable newsprint显示文摘 | Duff Sheldon J B Moritz John W Casavant Tracy E | 1995 | Biotechnology and Bioengineering1995，45，3： | 1 |
| 9 | Physical vapor transport growth of large AlN crystals 显示文摘 | TRACY W CHRIS C R SINGH N B | 2003 | J of Crystal Growth2003，250，1： | 1 |
| 10 | Determination of cotton nitrogen status with a hand-held chlorophyll meter显示文摘 | Wood C W Tracy P W Reeves D W | 1992 | J Plant Nutr1992，15，9： | 1 |
| 11 | Thickness and cell layer number of the pericarp of sweet corn and some of its relatives显示文摘 | Tracy W F Galinat W C | 1987 | Hortscience1987，22，4： | 1 |
| 12 | Developmental changes associated with divergent selection for rind penetrometer resistance in the MoSCSSS maize synthetic显示文摘 | Abedon B G Darrah L L Tracy W F | 1999 | Crop Science1999，39，： | 1 |
| 13 | Attention,Self~Control,and Health Behaviors显示文摘 | Traci M Andrew W | | 0，，05： | 1 |
| 14 | Graded response to thymectomy in children with myasthenia gravis显示文摘 | Tracy MM MeRae W Millichap JG | 2009 | J Child Neurol2009，24，4： | 1 |
| 15 | Comparison of canola oil conversion over MFI, BEA, and FAU显示文摘 | CHEN D TRACY N I CRUNKLETON D W | 2010 | Applied Catalysis A: General2010，384，12： | 1 |
| 16 | Effects of starchy sugary-2 and sugary sugary-2 endosperrm on pericarp thickness in sweet and waxy corn显示文摘 | Schmidt D H Tracy W F | 1998 | Hort Science1998，23，5： | 1 |
| 17 | Burnout and the Ethics of Self- Care for Therapists显示文摘 | TRACY L W | 2002 | Linking Research to Educational Practice Ⅱ2002，517，： | 1 |
| 18 | Effect of endosperm type on pericarp thickness in sweet corn inbreds显示文摘 | Tracy W F Schmidt K H | 1987 | Crop Sciences1987，27，1： | 1 |
| 19 | The transcriptionalprogram underlying the physiology of clostridial sporulation 显示文摘 | Jones S W Paredes C J Tracy B | 2008 | Genome Bio12008，9，7： | 1 |
| 20 | Purification and characterization of an enterotoxin from Bacteroides fragilis 显示文摘 | ROGER L V T DAVID M TRACY D W | 1992 | Infect Immun1992，60，4： | 1 |
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