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| 1 | A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals:A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis显示文摘The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.Moreover,these novel tools have become easier to use and have resulted in a great increase of applications.Whilst gene knockout(KO) or knockin(KI) animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these mutations.Although several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.The HMA-CE method uses a simple PCR amplification of genomic DNA(gDNA) followed by an automated capillary electrophoresis step which reveals a heteroduplexes(HD) signature for each mutation.This allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level. | Vanessa Chenouard Lucas Brusselle Jean-Marie Heslan Séverine Remy Séverine Ménoret Claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson | 2016 | Journal of Genetics and Genomics2016，43，5： | 4 |
| 2 | MicroRNAs and cell differentiation in mammalian development显示文摘 | Song L Tuan RS | 2006 | Birth Defects Res C Embryo Today2006，78，2： | 1 |
| 3 | Competitive framework for procurement of interruptible load services 显示文摘 | Tuan L A Bhattacharya K | 2003 | IEEE Transactions Power Systems2003，18，2： | 1 |
| 4 | EUTP: Energy-efficient and relia-ble transport protocol for data streaming in wireless sensor networks显示文摘 | TUAN L HU W CORKE P | 2009 | Computer Communications2009，32，78910： | 1 |
| 5 | Distinct functions of BMP4 and GDF5 in the regulation of chondrogenesis显示文摘 | Hatakeyama Y Tuan R S Shum L | 2004 | J Cell Biochem2004，91，6： | 1 |
| 6 | Competitive framework for procurement of interruptible load services显示文摘 | Tuan L A Bhattacharya K | 2003 | IEEE Transactions on Power Systems2003，18，2： | 1 |
| 7 | Leading to Learning and Competitive Intelligence显示文摘 | Tuan L T | 2013 | The Learning Organization2013，20，3： | 1 |
| 8 | Optimum dietary protein and lipid specifications for juvenile malabar grouper( Epinephelus malabari- cus) 显示文摘 | TUAN L A WILLIAMS K C | 2007 | Aquacuhure2007，267，1： | 1 |
| 9 | MicroRNAs and cell differentiation in mammalian development显示文摘 | Song L Tuan R S | 2006 | Birth Defects Res C Embryo Today2006，78，2： | 1 |
| 10 | Separation of light gas mixtures using SAPO-34membranes显示文摘 | Poshusta J C Tuan V A Pape E A Noble R D Falconer J L | 2000 | AIChE Journal2000，46，4： | 1 |
| 11 | Zirconium-SA- PO-11显示文摘 | Meriaudeau P Tuan V A Hung L N etal | 1997 | J Chem Soc Fraraday Trans1997，93，23： | 1 |
| 12 | Optimum dietary protein and lipid specifications for juvenile malabar grouper ( Epinephelus malabaricus) 显示文摘 | Tuan L A Williams K C | 2007 | Aquaculture2007，267，4： | 1 |
| 13 | Adult mesenchymal stem cells ; characterization, differentiation, and application in cell and gene therapy显示文摘 | Baksh D Song L Tuan RS | 2004 | J cell Mol Meal2004，8，3： | 1 |
| 14 | Separation of 1,3-propanediolfrom glycerol and glucose using a ZSM-5 zeolite membrane显示文摘 | LI S TUAN V A FALCONER J L | 2001 | J Membr Sci2001，191，1： | 1 |
| 15 | Adult mesenchymal stem cells:characterization,differentiation,and application in cell and gene therapy显示文摘 | BAKSH D SONG L TUAN RS | | 0，，03： | 1 |
| 16 | Optimum dietary pro-tein and lipid specifications for juvenile malabar grouper ( Epinephelus malabaricus)显示文摘 | TUAN L A WILLIAMS K C | 2007 | Aquaculture2007，267，1234： | 1 |
| 17 | X-type zeolite membranes: Preparation, characterization, and pervaporation performance显示文摘 | LI S TUAN V A FALCONER J L | 2002 | Mieroporous Mesoporous Mater2002，53，13： | 1 |
| 18 | Preparation of CuCl/C catalyst for the synthesis of dimethyl carbonate: Effects of calcination temperature显示文摘 | Tuan C L Chung S C | 2007 | Journal of the Chinese Institute of Chemical Engineers2007，38，： | 1 |
| 19 | Elastic behavior of a model twophase material显示文摘 | Hsieh C L Tuan W H Wu T T | 2004 | Journal of the European Ceramic Society2004，24，1516： | 1 |
| 20 | TNF-alpha stimulates acti- vation of pro-MMP2 in human skin through NF-(kappa) B me diated induction of MT1-MMP显示文摘 | Han Y P Tuan T L Wu H | 2001 | J Cell Sci2001，114，1： | 1 |
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