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| 1 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 2 | Phenotypic instability and rapid gene silencing in newly formed Arabidopsis alloteraploids显示文摘 | Comai L Tyagi A P Winter K Holmes-Davis R Reynolds S H Stevens Y Byers B | 2000 | Plant Cell2000，12，9： | 2 |
| 3 | Assessment of the efficiency of various gene promoters via biolistics in leaf and regenerating seed callus of millets,Eleusine coracana and Echinochloa crusgalli显示文摘 | GUPTA P RAGHUVANSHI S TYAGI A K | 2001 | Plant Biotechnol2001，18，： | 1 |
| 4 | Negative thermal expansion in Er2W3O12 and Yb2W3O12 by high temperature X-ray diffraction显示文摘 | Sumithra S Tyagi A K Umarji A M | 2005 | Mater Sci Eng B2005，116，： | 1 |
| 5 | Facile synthesis of ZrO2 powders:Control of morphology显示文摘 | Grover V Shukla R Tyagi A K | 2007 | Scr Mater2007，57，： | 1 |
| 6 | Solubility of Ce^4+ and Sr^2+ in the pyrochlore lattice of Gd2Zr2O7 for simulation of Pu and alkaline earth metal显示文摘 | PATWE S J TYAGI A K | 2006 | Ceram Intern2006，32，： | 1 |
| 7 | Silibinin down-regulates survivin protein and mRNA expression and causes caspases activation and apoptosis in human bladder transitional-cell papilloma RT4 cells显示文摘 | TYAGI A K AGARWAL C SINGH R P | 2003 | Biochem Biophys Res Comm2003，312，4： | 1 |
| 8 | Powder characteristics and sinterability of ceria powders prepared through different routes显示文摘 | Purohit R D Saha S Tyagi A K | 2006 | Ceram Int2006，32，： | 1 |
| 9 | 显示文摘 | Kim H J Tyagi R K Fouda A E | 1996 | J Appl Polym Sci1996，62，： | 1 |
| 10 | Tuberculosis DNA fragment contains genes encoding cell division proteins ftsX and ftsE, a basic protein and homo- logues of PernK and small protein B显示文摘 | Tyagi J S Das T K Kinger A K | 1996 | Gene1996，177，1： | 1 |
| 11 | Di-and tetranuclear ruthenium(Ⅱ)and/or osmium(Ⅱ)complexes of polypyridyl ligands bridged by a fully conjugated aromatic spacer:synthesis,characterization,and electrochemical and photophysical studies 显示文摘 | BILAKHIYA A K TYAGI B PAUL P | 2002 | Inorg Chem2002，41，15： | 1 |
| 12 | Physiology and molecular biology of salinitystress tolerance in plants显示文摘 | SAIRAM R K TYAGI A | 2004 | Current Advances in Plant Science2004，86，： | 1 |
| 13 | Energy and Exergy Analysis of Typical Renewable Energy Systems 显示文摘 | PARK S R PANDEY A K TYAGI V V | 2014 | Renewable and Sustainable Energy Reviews2014，30，2： | 1 |
| 14 | Validation of housekeeping genes as internal control for studying gene expression in rice by quantitative real-time PCR显示文摘 | JAIN M NIJHAWAN A TYAGI A K | 2006 | Biochem Biophys Res Commun2006，345，2： | 1 |
| 15 | Antialgal activity of a hepatoxin-producing cyanbacterium, Microcystis aeruginosa 显示文摘 | Singh D P Tyagi M B Kumar A Thakur J K Kumar A | 2001 | World J Microbiol Biotechnol2001，17，： | 1 |
| 16 | Validation of housekeeping genes as internal control for studying gene expression irt rice by quantitative real-time PCR 显示文摘 | Jain M Niihawan A Tyagi A K | 2006 | Biochemical and Biophysical Research Communications2006，345，： | 1 |
| 17 | Diet- ary feeding of silibinin inhibits advance human prostate car- cinoma growth in athymic nude mice and increases plasma insulin-like growth factor-binding protein-3 levels显示文摘 | SINGH RP DHANALAKSHMI S TYAGI A K | 2002 | Cancer Res2002，62，11： | 1 |
| 18 | Developmental and light-dependent cues interact to establish steady-state levels of transcripts for photosynthe- sis-related genes(psbA, psbD, psaA and rbcL) in rice(Oryza sativa L,) 显示文摘 | Kapoor S Maheshwari S C Tyagi A K | 1994 | Curr Genet1994，25，： | 1 |
| 19 | Phase transition and negative thermal expansion in A2(MOO4)3 system (A= Fe^3+, Cr^3+, Al^3+) 显示文摘 | Tyagi A K Achary S N Mathews M D | 2002 | J Alloys Compd2002，339，： | 1 |
| 20 | Analysis of expression profile of selected genes expressed during auxininduced somatic embryogenesis in leaf base system of wheat (Triticum aestivum) and their possible interactions 显示文摘 | Singla B Tyagi A K Khurana J P | 2007 | Plant Molecular Biology2007，65，5： | 1 |
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