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| 1 | Agrobacterium-mediated transformation and assessment of factors influ-encing transgene expression in loblolly pine (Pinus taeda L.)显示文摘This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for β-glucuronidase (GUS) and neomycin phosphotransferase (NPTII). Factors influencing transgene expression including seed sources of loblolly pine, concentration of bacteria, and the wounding procedures of target explants were investigated. The expression of foreign gene was confirmed by the ability of mature zygotic embryos to produce calli in the presence of kanamycin, by histochemical assays of GUS activity, by PCR analysis, and by Southern blot. The successful expression of the GUS gene in different families of loblolly pine suggests that this transformation system is probably useful for the production of the genetically modified conifers. | TANG WEI North Carolina State University, 2900 Ligon Sf., Raleigh, NC 27607, USA | 2001 | Cell Research2001，11，3： | 3 |
| 2 | Diagnoses of newborns and mothers with carnitine uptake defects through newborn screening显示文摘 | Lee NC Tang NL Chien YH | 2010 | Mol Genet Metab2010，100，1： | 1 |
| 3 | Well-defined biocompatible blook copolymers via atom transfer radical polymerization of 2-methacryloyloxyethyl phosphorylcholine in protic media显示文摘 | Ma YH Tang YQ Billingham NC | 2003 | Macromolecules2003，36，10： | 1 |
| 4 | Proteasome inhibi- tion promotes regression of left ventricular hypertrophy 显示文摘 | Stansfield WE Tang RH Moss NC | 2008 | Am J Physiol Heart Circ Physiol2008，294，2： | 1 |
| 5 | Proteasome inhibition promotes regression of left ventricular hypertrophy显示文摘 | Stansfield WE Tang RH Moss NC | | 0，，02： | 1 |
| 6 | Resveratrol inhibits mi gration and invasion of human breast-cancer cells显示文摘 | Tang FY Su YC Chen NC | 2008 | Mol Nutr Food Res2008，52，6： | 1 |
| 7 | Restoration of basic hand function by double transpositional digital replantation in five-digit amputations 显示文摘 | Chextg TJ Cheng NC Tang YB | 2004 | J Reconstr Microsurg2004，20，3： | 1 |
| 8 | Diagnoses of newborns and mothers with earnitine uptake defects through newborn screening 显示文摘 | Lee NC Tang NL Chien YH | 2010 | Mol Genet Metab2010，100，： | 1 |
| 9 | Adenylyl cyclase type 6 deletion decreases left ventricular function via impaired calcium handling显示文摘 | Tang T Gao MH Lai NC | 2008 | Circulation2008，117，： | 1 |
| 10 | Neonatal bacteremia in a neonatal intensive care unit: analysis of causative organisms and antimicrobial susceptibility 显示文摘 | Lee NC Chen SJ Tang RB | 2004 | J Chin Med Assoc2004，67，1： | 1 |
| 11 | Improved function of the failing rat heart by regulated expression of insulin - like growth factor I via intramuscular gene transfer 显示文摘 | Lai NC Tang T Gao MH | 2012 | Hum Gene Ther2012，23，3： | 1 |
| 12 | Serodiagnosis of Penicillium marneffei显示文摘 | Yuen KY Wong SY Tang NC | 1994 | Lancet1994，344，8290： | 1 |
| 13 | Resveratrol inhibits mi- gration and invasion of human breast-cancer cells显示文摘 | Tang FY Yc S Chen NC | 2008 | Mol Nutr Food Res2008，52，6： | 1 |
| 14 | Increased cardiac adenylyl cyclase expression is associated with increased survival after myocardial infarction 显示文摘 | Takahashi T Tang T Lai NC | 2006 | Circulation2006，114，5： | 1 |
| 15 | Molecular Mechanism of Salinity Stress and Biotechnological Strategies for Engineering Salt Tolerance in Plants显示文摘Molecular mechanisms of plant responses to salinity stress and the physiological consequences of altered gene expression are reviewed. Extensive use of comparisons with halophytic plants and glycophytos provide a paradigm for many responses to salinity exhibited by stress sensitive plants. Osmolyte biosynthesis, water flux control, and membrane transport of ions are important for maintenance and re-establishment of homeostasis. Transgenic plant and mutant analyses in Arabidopsis improve the understanding of stress responses and elements of stress signal transduction pathways. The genomic DNA sequences and cell-specific transcript expression data, combined with determinant identification based on molecular genetics, will provide the infrastructure for functional physiological dissection of salt tolerance determinants in plants. Protein interaction analysis, genetic activation and suppression screens will lead inevitably to an understanding of the interrelationships of the multiple signaling systems that control stress-adaptive responses and provide more opportunity to engineer salt tolerance in plants. | Wei Tang* Latoya Harris Ronald J. Newton Department of Biology, Howell Science Complex, East Carolina University, Greenville, NC 27858-4353, USA | 2003 | Forestry Studies in China2003，5，2： | 1 |
| 16 | Activation of cardiac adenylyl cyclase expression increases function of the failing ischemic heart in mice 显示文摘 | Lai NC Tang T Gao MH | 2008 | J Am College Cardiol2008，51，15： | 1 |
| 17 | Diagnoses of newborns and mothers with carnitine uptake defects through newborn screening显示文摘 | Lee NC Tang NL Chien YH | 2010 | Mol Genet Metab2010，100，1： | 1 |
| 18 | Current clinical evidence for the use of mesenchymal stern cells in articular cartilage repair显示文摘 | Reissis D Tang QO Cooper NC | 2016 | Expert Opin Biol ther2016，16，4： | 1 |
| 19 | Proteasome inhibition promotes regression of left ventricular hypeitrophy显示文摘 | Stansfield WE Tang RH Moss NC | 2008 | Am J Physiol Heart Cite Physiol2008，294，2： | 1 |
| 20 | Resveratrol inhibits migration and invasion of human breast-cancer cells显示文摘 | Tang FY Su YC Chen NC | 2008 | Mol Nutr Food Res2008，52，6： | 1 |
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